Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0340 and EX28DRAFT_0341 overlap by 4 nucleotides EX28DRAFT_0341 and EX28DRAFT_0342 overlap by 4 nucleotides EX28DRAFT_0342 and EX28DRAFT_0343 overlap by 1 nucleotides
EX28DRAFT_0340: EX28DRAFT_0340 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase, at 302,000 to 303,670
_0340
EX28DRAFT_0341: EX28DRAFT_0341 - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase, at 303,667 to 304,443
_0341
EX28DRAFT_0342: EX28DRAFT_0342 - 1,4-Dihydroxy-2-naphthoyl-CoA synthase (EC 4.1.3.36), at 304,440 to 305,297
_0342
EX28DRAFT_0343: EX28DRAFT_0343 - O-succinylbenzoate synthase (EC 4.2.1.113), at 305,297 to 306,262
_0343
Position (kb) 303
304
305 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 302.915 kb on + strand, within EX28DRAFT_0340 at 303.521 kb on + strand at 303.797 kb on - strand, within EX28DRAFT_0341 at 304.108 kb on + strand, within EX28DRAFT_0341 at 304.277 kb on - strand, within EX28DRAFT_0341 at 304.277 kb on - strand, within EX28DRAFT_0341 at 304.439 kb on + strand at 304.439 kb on + strand at 304.439 kb on + strand at 304.440 kb on - strand at 304.440 kb on - strand at 304.446 kb on + strand at 304.916 kb on - strand, within EX28DRAFT_0342 at 304.947 kb on + strand, within EX28DRAFT_0342 at 305.007 kb on + strand, within EX28DRAFT_0342 at 305.211 kb on + strand, within EX28DRAFT_0342 at 305.212 kb on - strand at 305.280 kb on + strand at 305.280 kb on + strand at 305.281 kb on - strand at 305.281 kb on - strand at 305.281 kb on - strand at 305.296 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 302,915 + EX28DRAFT_0340 0.55 -0.5 303,521 + +0.1 303,797 - EX28DRAFT_0341 0.17 -0.3 304,108 + EX28DRAFT_0341 0.57 +1.9 304,277 - EX28DRAFT_0341 0.79 +1.2 304,277 - EX28DRAFT_0341 0.79 +0.8 304,439 + -3.5 304,439 + +0.2 304,439 + -1.7 304,440 - -0.0 304,440 - +1.1 304,446 + -0.5 304,916 - EX28DRAFT_0342 0.55 +0.2 304,947 + EX28DRAFT_0342 0.59 +0.2 305,007 + EX28DRAFT_0342 0.66 +1.0 305,211 + EX28DRAFT_0342 0.90 +0.4 305,212 - -0.0 305,280 + +0.2 305,280 + -0.7 305,281 - +1.8 305,281 - +1.2 305,281 - +0.7 305,296 - -0.4
Or see this region's nucleotide sequence