Experiment: without_iron3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20465 and AMB_RS20470 overlap by 4 nucleotides AMB_RS20470 and AMB_RS23635 are separated by 2 nucleotides AMB_RS23635 and AMB_RS20480 are separated by 28 nucleotides AMB_RS20480 and AMB_RS20485 overlap by 14 nucleotides AMB_RS20485 and AMB_RS20490 are separated by 8 nucleotides AMB_RS20490 and AMB_RS20495 are separated by 119 nucleotides
AMB_RS20465: AMB_RS20465 - hypothetical protein, at 4,447,650 to 4,448,309
_RS20465
AMB_RS20470: AMB_RS20470 - hypothetical protein, at 4,448,306 to 4,449,235
_RS20470
AMB_RS23635: AMB_RS23635 - hypothetical protein, at 4,449,238 to 4,449,720
_RS23635
AMB_RS20480: AMB_RS20480 - hypothetical protein, at 4,449,749 to 4,450,054
_RS20480
AMB_RS20485: AMB_RS20485 - hypothetical protein, at 4,450,041 to 4,450,238
_RS20485
AMB_RS20490: AMB_RS20490 - hypothetical protein, at 4,450,247 to 4,450,510
_RS20490
AMB_RS20495: AMB_RS20495 - helix-turn-helix domain-containing protein, at 4,450,630 to 4,450,812
_RS20495
Position (kb)
4449
4450 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4448.301 kb on + strand at 4448.301 kb on + strand at 4448.302 kb on - strand at 4448.302 kb on - strand at 4448.302 kb on - strand at 4448.302 kb on - strand at 4448.611 kb on + strand, within AMB_RS20470 at 4448.639 kb on + strand, within AMB_RS20470 at 4448.639 kb on + strand, within AMB_RS20470 at 4448.639 kb on + strand, within AMB_RS20470 at 4448.639 kb on + strand, within AMB_RS20470 at 4448.640 kb on - strand, within AMB_RS20470 at 4448.640 kb on - strand, within AMB_RS20470 at 4449.008 kb on + strand, within AMB_RS20470 at 4449.008 kb on + strand, within AMB_RS20470 at 4449.009 kb on - strand, within AMB_RS20470 at 4449.009 kb on - strand, within AMB_RS20470 at 4449.009 kb on - strand, within AMB_RS20470 at 4449.041 kb on + strand, within AMB_RS20470 at 4449.041 kb on + strand, within AMB_RS20470 at 4449.042 kb on - strand, within AMB_RS20470 at 4449.042 kb on - strand, within AMB_RS20470 at 4449.042 kb on - strand, within AMB_RS20470 at 4449.042 kb on - strand, within AMB_RS20470 at 4449.062 kb on + strand, within AMB_RS20470 at 4449.062 kb on + strand, within AMB_RS20470 at 4449.063 kb on - strand, within AMB_RS20470 at 4449.063 kb on - strand, within AMB_RS20470 at 4449.318 kb on + strand, within AMB_RS23635 at 4449.343 kb on + strand, within AMB_RS23635 at 4449.344 kb on - strand, within AMB_RS23635 at 4449.469 kb on + strand, within AMB_RS23635 at 4449.469 kb on + strand, within AMB_RS23635 at 4449.469 kb on + strand, within AMB_RS23635 at 4449.470 kb on - strand, within AMB_RS23635 at 4449.470 kb on - strand, within AMB_RS23635 at 4449.470 kb on - strand, within AMB_RS23635 at 4449.483 kb on - strand, within AMB_RS23635 at 4449.483 kb on - strand, within AMB_RS23635 at 4449.483 kb on - strand, within AMB_RS23635 at 4449.541 kb on + strand, within AMB_RS23635 at 4449.541 kb on + strand, within AMB_RS23635 at 4449.541 kb on + strand, within AMB_RS23635 at 4449.541 kb on + strand, within AMB_RS23635 at 4449.541 kb on + strand, within AMB_RS23635 at 4449.541 kb on + strand, within AMB_RS23635 at 4449.622 kb on + strand, within AMB_RS23635 at 4449.623 kb on - strand, within AMB_RS23635 at 4449.794 kb on + strand, within AMB_RS20480 at 4449.794 kb on + strand, within AMB_RS20480 at 4449.794 kb on + strand, within AMB_RS20480 at 4449.794 kb on + strand, within AMB_RS20480 at 4449.795 kb on - strand, within AMB_RS20480 at 4449.795 kb on - strand, within AMB_RS20480 at 4449.795 kb on - strand, within AMB_RS20480 at 4449.795 kb on - strand, within AMB_RS20480 at 4449.857 kb on + strand, within AMB_RS20480 at 4449.858 kb on - strand, within AMB_RS20480 at 4449.914 kb on + strand, within AMB_RS20480 at 4450.475 kb on + strand, within AMB_RS20490 at 4450.475 kb on + strand, within AMB_RS20490 at 4450.476 kb on - strand, within AMB_RS20490 at 4450.559 kb on + strand at 4450.559 kb on + strand at 4450.559 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.649 kb on - strand, within AMB_RS20495 at 4450.649 kb on - strand, within AMB_RS20495 at 4450.649 kb on - strand, within AMB_RS20495 at 4450.649 kb on - strand, within AMB_RS20495
Per-strain Table
Position Strand Gene LocusTag Fraction without_iron3 remove 4,448,301 + +0.9 4,448,301 + +0.3 4,448,302 - -0.1 4,448,302 - +0.6 4,448,302 - +0.7 4,448,302 - -1.0 4,448,611 + AMB_RS20470 0.33 +0.2 4,448,639 + AMB_RS20470 0.36 -1.4 4,448,639 + AMB_RS20470 0.36 -0.5 4,448,639 + AMB_RS20470 0.36 -0.4 4,448,639 + AMB_RS20470 0.36 -0.8 4,448,640 - AMB_RS20470 0.36 -1.1 4,448,640 - AMB_RS20470 0.36 +0.8 4,449,008 + AMB_RS20470 0.75 +0.0 4,449,008 + AMB_RS20470 0.75 -1.6 4,449,009 - AMB_RS20470 0.76 -0.8 4,449,009 - AMB_RS20470 0.76 -0.2 4,449,009 - AMB_RS20470 0.76 -1.0 4,449,041 + AMB_RS20470 0.79 +0.0 4,449,041 + AMB_RS20470 0.79 -0.3 4,449,042 - AMB_RS20470 0.79 -1.0 4,449,042 - AMB_RS20470 0.79 -0.2 4,449,042 - AMB_RS20470 0.79 +0.6 4,449,042 - AMB_RS20470 0.79 -0.1 4,449,062 + AMB_RS20470 0.81 -0.1 4,449,062 + AMB_RS20470 0.81 -0.4 4,449,063 - AMB_RS20470 0.81 -0.3 4,449,063 - AMB_RS20470 0.81 +0.3 4,449,318 + AMB_RS23635 0.17 -0.7 4,449,343 + AMB_RS23635 0.22 -0.5 4,449,344 - AMB_RS23635 0.22 -1.6 4,449,469 + AMB_RS23635 0.48 +0.7 4,449,469 + AMB_RS23635 0.48 -0.2 4,449,469 + AMB_RS23635 0.48 -1.7 4,449,470 - AMB_RS23635 0.48 +1.4 4,449,470 - AMB_RS23635 0.48 -1.2 4,449,470 - AMB_RS23635 0.48 -0.2 4,449,483 - AMB_RS23635 0.51 +2.2 4,449,483 - AMB_RS23635 0.51 +0.2 4,449,483 - AMB_RS23635 0.51 -0.6 4,449,541 + AMB_RS23635 0.63 -0.7 4,449,541 + AMB_RS23635 0.63 +0.2 4,449,541 + AMB_RS23635 0.63 -1.1 4,449,541 + AMB_RS23635 0.63 -0.0 4,449,541 + AMB_RS23635 0.63 +1.7 4,449,541 + AMB_RS23635 0.63 +1.0 4,449,622 + AMB_RS23635 0.80 +0.9 4,449,623 - AMB_RS23635 0.80 +0.4 4,449,794 + AMB_RS20480 0.15 -0.2 4,449,794 + AMB_RS20480 0.15 -0.2 4,449,794 + AMB_RS20480 0.15 -0.2 4,449,794 + AMB_RS20480 0.15 +2.0 4,449,795 - AMB_RS20480 0.15 +0.4 4,449,795 - AMB_RS20480 0.15 +0.1 4,449,795 - AMB_RS20480 0.15 -0.1 4,449,795 - AMB_RS20480 0.15 -1.1 4,449,857 + AMB_RS20480 0.35 -1.0 4,449,858 - AMB_RS20480 0.36 -0.6 4,449,914 + AMB_RS20480 0.54 -2.1 4,450,475 + AMB_RS20490 0.86 -0.5 4,450,475 + AMB_RS20490 0.86 -0.6 4,450,476 - AMB_RS20490 0.87 -0.7 4,450,559 + -0.1 4,450,559 + -0.7 4,450,559 + -0.5 4,450,648 + -1.3 4,450,648 + -0.4 4,450,648 + +0.2 4,450,648 + -0.4 4,450,648 + -0.4 4,450,648 + +1.0 4,450,648 + -0.3 4,450,648 + +0.0 4,450,649 - AMB_RS20495 0.10 -0.3 4,450,649 - AMB_RS20495 0.10 -2.7 4,450,649 - AMB_RS20495 0.10 +0.3 4,450,649 - AMB_RS20495 0.10 +2.4
Or see this region's nucleotide sequence