Experiment: without_iron3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS08310 and AMB_RS08315 overlap by 1 nucleotides AMB_RS08315 and AMB_RS08320 overlap by 8 nucleotides AMB_RS08320 and AMB_RS08325 overlap by 1 nucleotides AMB_RS08325 and AMB_RS08330 are separated by 2 nucleotides AMB_RS08330 and AMB_RS08335 are separated by 59 nucleotides
AMB_RS08310: AMB_RS08310 - hydrogenase accessory protein HypB, at 1,780,511 to 1,781,449
_RS08310
AMB_RS08315: AMB_RS08315 - hydrogenase maturation nickel metallochaperone HypA, at 1,781,449 to 1,781,793
_RS08315
AMB_RS08320: AMB_RS08320 - Ni,Fe-hydrogenase I large subunit, at 1,781,786 to 1,782,889
_RS08320
AMB_RS08325: AMB_RS08325 - hydrogenase maturation factor, at 1,782,889 to 1,783,122
_RS08325
AMB_RS08330: AMB_RS08330 - HyaD/HybD family hydrogenase maturation endopeptidase, at 1,783,125 to 1,783,607
_RS08330
AMB_RS08335: AMB_RS08335 - nickel-dependent hydrogenase large subunit, at 1,783,667 to 1,785,370
_RS08335
Position (kb)
1781
1782
1783 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1780.830 kb on + strand, within AMB_RS08310 at 1780.830 kb on + strand, within AMB_RS08310 at 1780.830 kb on + strand, within AMB_RS08310 at 1780.831 kb on - strand, within AMB_RS08310 at 1780.831 kb on - strand, within AMB_RS08310 at 1780.831 kb on - strand, within AMB_RS08310 at 1780.831 kb on - strand, within AMB_RS08310 at 1780.939 kb on + strand, within AMB_RS08310 at 1780.939 kb on + strand, within AMB_RS08310 at 1780.939 kb on + strand, within AMB_RS08310 at 1780.940 kb on - strand, within AMB_RS08310 at 1781.274 kb on + strand, within AMB_RS08310 at 1781.764 kb on + strand at 1781.765 kb on - strand at 1781.765 kb on - strand at 1781.765 kb on - strand at 1781.950 kb on - strand, within AMB_RS08320 at 1782.159 kb on + strand, within AMB_RS08320 at 1782.736 kb on - strand, within AMB_RS08320 at 1782.923 kb on + strand, within AMB_RS08325 at 1782.923 kb on + strand, within AMB_RS08325 at 1782.923 kb on + strand, within AMB_RS08325 at 1782.924 kb on - strand, within AMB_RS08325 at 1782.924 kb on - strand, within AMB_RS08325 at 1782.924 kb on - strand, within AMB_RS08325 at 1783.519 kb on + strand, within AMB_RS08330 at 1783.519 kb on + strand, within AMB_RS08330 at 1783.519 kb on + strand, within AMB_RS08330 at 1783.519 kb on + strand, within AMB_RS08330 at 1783.520 kb on - strand, within AMB_RS08330 at 1783.520 kb on - strand, within AMB_RS08330 at 1783.662 kb on + strand at 1783.662 kb on + strand at 1783.663 kb on - strand at 1783.668 kb on + strand at 1783.669 kb on - strand at 1783.669 kb on - strand at 1783.669 kb on - strand at 1783.669 kb on - strand at 1783.815 kb on + strand at 1783.815 kb on + strand at 1783.816 kb on - strand at 1783.826 kb on - strand at 1783.826 kb on - strand at 1783.826 kb on - strand at 1783.881 kb on + strand, within AMB_RS08335 at 1783.881 kb on + strand, within AMB_RS08335 at 1783.882 kb on - strand, within AMB_RS08335 at 1783.882 kb on - strand, within AMB_RS08335 at 1783.882 kb on - strand, within AMB_RS08335 at 1783.882 kb on - strand, within AMB_RS08335
Per-strain Table
Position Strand Gene LocusTag Fraction without_iron3 remove 1,780,830 + AMB_RS08310 0.34 +0.7 1,780,830 + AMB_RS08310 0.34 +0.4 1,780,830 + AMB_RS08310 0.34 -0.3 1,780,831 - AMB_RS08310 0.34 -0.1 1,780,831 - AMB_RS08310 0.34 +0.8 1,780,831 - AMB_RS08310 0.34 +0.5 1,780,831 - AMB_RS08310 0.34 -0.2 1,780,939 + AMB_RS08310 0.46 +0.1 1,780,939 + AMB_RS08310 0.46 +0.2 1,780,939 + AMB_RS08310 0.46 +0.2 1,780,940 - AMB_RS08310 0.46 -0.2 1,781,274 + AMB_RS08310 0.81 -1.1 1,781,764 + +1.8 1,781,765 - -0.8 1,781,765 - +0.3 1,781,765 - +0.2 1,781,950 - AMB_RS08320 0.15 -0.2 1,782,159 + AMB_RS08320 0.34 -0.8 1,782,736 - AMB_RS08320 0.86 -0.9 1,782,923 + AMB_RS08325 0.15 -0.4 1,782,923 + AMB_RS08325 0.15 +1.8 1,782,923 + AMB_RS08325 0.15 -1.1 1,782,924 - AMB_RS08325 0.15 -0.1 1,782,924 - AMB_RS08325 0.15 -0.3 1,782,924 - AMB_RS08325 0.15 -0.8 1,783,519 + AMB_RS08330 0.82 -0.1 1,783,519 + AMB_RS08330 0.82 -2.4 1,783,519 + AMB_RS08330 0.82 -0.1 1,783,519 + AMB_RS08330 0.82 -0.8 1,783,520 - AMB_RS08330 0.82 -0.1 1,783,520 - AMB_RS08330 0.82 +0.1 1,783,662 + +0.0 1,783,662 + -0.5 1,783,663 - -1.8 1,783,668 + +0.2 1,783,669 - -0.4 1,783,669 - +0.0 1,783,669 - +0.4 1,783,669 - -0.4 1,783,815 + -3.1 1,783,815 + +0.1 1,783,816 - -0.1 1,783,826 - -0.4 1,783,826 - -0.7 1,783,826 - +0.0 1,783,881 + AMB_RS08335 0.13 +0.4 1,783,881 + AMB_RS08335 0.13 -0.4 1,783,882 - AMB_RS08335 0.13 -0.1 1,783,882 - AMB_RS08335 0.13 -0.0 1,783,882 - AMB_RS08335 0.13 -0.1 1,783,882 - AMB_RS08335 0.13 -0.2
Or see this region's nucleotide sequence