Experiment: Nickel (II) chloride 80 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_17810 and IAI46_17815 are separated by 21 nucleotides IAI46_17815 and IAI46_17820 are separated by 232 nucleotides IAI46_17820 and IAI46_17825 are separated by 0 nucleotides
IAI46_17810: IAI46_17810 - amidophosphoribosyltransferase, at 3,752,964 to 3,754,481
_17810
IAI46_17815: IAI46_17815 - colicin V production protein, at 3,754,503 to 3,755,003
_17815
IAI46_17820: IAI46_17820 - cell division protein DedD, at 3,755,236 to 3,756,000
_17820
IAI46_17825: IAI46_17825 - bifunctional tetrahydrofolate synthase/dihydrofolate synthase, at 3,756,001 to 3,757,266
_17825
Position (kb)
3755
3756
3757 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3754.301 kb on + strand, within IAI46_17810 at 3754.301 kb on + strand, within IAI46_17810 at 3754.411 kb on - strand at 3754.411 kb on - strand at 3754.430 kb on - strand at 3754.472 kb on + strand at 3754.516 kb on - strand at 3754.553 kb on - strand, within IAI46_17815 at 3754.577 kb on - strand, within IAI46_17815 at 3754.592 kb on + strand, within IAI46_17815 at 3754.672 kb on - strand, within IAI46_17815 at 3754.752 kb on - strand, within IAI46_17815 at 3754.752 kb on - strand, within IAI46_17815 at 3754.759 kb on + strand, within IAI46_17815 at 3754.759 kb on + strand, within IAI46_17815 at 3754.760 kb on - strand, within IAI46_17815 at 3754.760 kb on - strand, within IAI46_17815 at 3754.760 kb on - strand, within IAI46_17815 at 3754.764 kb on - strand, within IAI46_17815 at 3754.764 kb on - strand, within IAI46_17815 at 3754.769 kb on + strand, within IAI46_17815 at 3754.769 kb on + strand, within IAI46_17815 at 3754.769 kb on + strand, within IAI46_17815 at 3754.770 kb on - strand, within IAI46_17815 at 3754.777 kb on - strand, within IAI46_17815 at 3754.777 kb on - strand, within IAI46_17815 at 3754.853 kb on - strand, within IAI46_17815 at 3754.864 kb on + strand, within IAI46_17815 at 3754.977 kb on + strand at 3754.978 kb on - strand at 3754.978 kb on - strand at 3754.978 kb on - strand at 3754.980 kb on - strand at 3754.988 kb on - strand at 3755.008 kb on + strand at 3755.009 kb on - strand at 3755.097 kb on + strand at 3755.104 kb on - strand at 3755.145 kb on + strand at 3755.145 kb on + strand at 3755.146 kb on - strand at 3755.146 kb on - strand at 3755.159 kb on + strand at 3755.186 kb on + strand at 3755.187 kb on - strand at 3755.187 kb on - strand at 3755.189 kb on - strand at 3755.242 kb on + strand at 3755.339 kb on + strand, within IAI46_17820 at 3755.339 kb on + strand, within IAI46_17820 at 3755.468 kb on + strand, within IAI46_17820 at 3755.468 kb on + strand, within IAI46_17820 at 3755.468 kb on + strand, within IAI46_17820 at 3755.468 kb on + strand, within IAI46_17820 at 3755.469 kb on - strand, within IAI46_17820 at 3755.469 kb on - strand, within IAI46_17820 at 3755.469 kb on - strand, within IAI46_17820 at 3755.469 kb on - strand, within IAI46_17820 at 3755.901 kb on - strand, within IAI46_17820 at 3755.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, anaerobic remove 3,754,301 + IAI46_17810 0.88 -2.1 3,754,301 + IAI46_17810 0.88 -0.9 3,754,411 - -1.5 3,754,411 - -3.8 3,754,430 - -2.0 3,754,472 + +0.3 3,754,516 - -1.3 3,754,553 - IAI46_17815 0.10 +0.8 3,754,577 - IAI46_17815 0.15 -0.0 3,754,592 + IAI46_17815 0.18 -1.3 3,754,672 - IAI46_17815 0.34 +0.3 3,754,752 - IAI46_17815 0.50 -0.4 3,754,752 - IAI46_17815 0.50 -0.1 3,754,759 + IAI46_17815 0.51 -1.1 3,754,759 + IAI46_17815 0.51 -1.6 3,754,760 - IAI46_17815 0.51 +0.4 3,754,760 - IAI46_17815 0.51 -0.9 3,754,760 - IAI46_17815 0.51 +0.4 3,754,764 - IAI46_17815 0.52 +0.0 3,754,764 - IAI46_17815 0.52 +0.1 3,754,769 + IAI46_17815 0.53 -0.6 3,754,769 + IAI46_17815 0.53 -0.6 3,754,769 + IAI46_17815 0.53 -2.1 3,754,770 - IAI46_17815 0.53 +0.6 3,754,777 - IAI46_17815 0.55 +1.0 3,754,777 - IAI46_17815 0.55 -0.3 3,754,853 - IAI46_17815 0.70 +0.1 3,754,864 + IAI46_17815 0.72 -0.9 3,754,977 + -0.9 3,754,978 - +0.4 3,754,978 - +0.5 3,754,978 - +0.1 3,754,980 - -0.4 3,754,988 - -0.7 3,755,008 + -0.7 3,755,009 - -1.2 3,755,097 + -0.3 3,755,104 - -0.1 3,755,145 + +0.9 3,755,145 + -0.5 3,755,146 - -0.8 3,755,146 - +1.9 3,755,159 + +0.7 3,755,186 + +0.3 3,755,187 - -0.4 3,755,187 - +0.1 3,755,189 - +0.5 3,755,242 + -1.3 3,755,339 + IAI46_17820 0.13 -0.4 3,755,339 + IAI46_17820 0.13 -0.7 3,755,468 + IAI46_17820 0.30 +0.2 3,755,468 + IAI46_17820 0.30 +1.2 3,755,468 + IAI46_17820 0.30 -1.1 3,755,468 + IAI46_17820 0.30 -1.0 3,755,469 - IAI46_17820 0.30 +0.4 3,755,469 - IAI46_17820 0.30 +0.3 3,755,469 - IAI46_17820 0.30 -0.1 3,755,469 - IAI46_17820 0.30 -0.1 3,755,901 - IAI46_17820 0.87 +0.1 3,755,992 - -0.7
Or see this region's nucleotide sequence