Experiment: Nickel (II) chloride 80 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_14560 and IAI46_14565 overlap by 14 nucleotides IAI46_14565 and IAI46_14570 are separated by 565 nucleotides IAI46_14570 and IAI46_14575 are separated by 154 nucleotides
IAI46_14560: IAI46_14560 - multidrug/spermidine efflux SMR transporter subunit MdtI, at 3,086,841 to 3,087,170
_14560
IAI46_14565: IAI46_14565 - multidrug/spermidine efflux SMR transporter subunit MdtJ, at 3,087,157 to 3,087,525
_14565
IAI46_14570: IAI46_14570 - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 3,088,091 to 3,088,732
_14570
IAI46_14575: IAI46_14575 - glucose-6-phosphate dehydrogenase, at 3,088,887 to 3,090,362
_14575
Position (kb)
3088
3089 Strain fitness (log2 ratio)
-1
0
1 at 3087.151 kb on + strand at 3087.316 kb on - strand, within IAI46_14565 at 3087.373 kb on - strand, within IAI46_14565 at 3087.395 kb on + strand, within IAI46_14565 at 3087.395 kb on + strand, within IAI46_14565 at 3087.396 kb on - strand, within IAI46_14565 at 3087.396 kb on - strand, within IAI46_14565 at 3087.416 kb on + strand, within IAI46_14565 at 3087.417 kb on - strand, within IAI46_14565 at 3087.417 kb on - strand, within IAI46_14565 at 3087.426 kb on + strand, within IAI46_14565 at 3087.519 kb on - strand at 3087.549 kb on - strand at 3087.558 kb on + strand at 3087.730 kb on - strand at 3087.730 kb on - strand at 3087.730 kb on - strand at 3087.825 kb on - strand at 3087.829 kb on - strand at 3087.836 kb on - strand at 3087.860 kb on + strand at 3087.861 kb on - strand at 3088.117 kb on + strand at 3088.228 kb on - strand, within IAI46_14570 at 3088.387 kb on + strand, within IAI46_14570 at 3088.388 kb on - strand, within IAI46_14570 at 3088.817 kb on - strand at 3088.869 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, anaerobic remove 3,087,151 + +0.5 3,087,316 - IAI46_14565 0.43 -0.1 3,087,373 - IAI46_14565 0.59 -0.1 3,087,395 + IAI46_14565 0.64 -0.3 3,087,395 + IAI46_14565 0.64 -0.1 3,087,396 - IAI46_14565 0.65 +0.3 3,087,396 - IAI46_14565 0.65 +0.3 3,087,416 + IAI46_14565 0.70 +0.6 3,087,417 - IAI46_14565 0.70 +0.5 3,087,417 - IAI46_14565 0.70 +0.1 3,087,426 + IAI46_14565 0.73 +0.3 3,087,519 - +0.8 3,087,549 - +0.3 3,087,558 + -0.7 3,087,730 - +0.9 3,087,730 - +1.1 3,087,730 - -0.9 3,087,825 - +0.6 3,087,829 - +0.2 3,087,836 - -0.2 3,087,860 + +0.1 3,087,861 - +0.3 3,088,117 + +0.3 3,088,228 - IAI46_14570 0.21 -0.7 3,088,387 + IAI46_14570 0.46 -0.8 3,088,388 - IAI46_14570 0.46 -0.3 3,088,817 - +1.2 3,088,869 - -0.5
Or see this region's nucleotide sequence