Experiment: Nickel (II) chloride 80 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_04930 and IAI46_04935 are separated by 5 nucleotides IAI46_04935 and IAI46_04940 are separated by 232 nucleotides IAI46_04940 and IAI46_04945 are separated by 33 nucleotides IAI46_04945 and IAI46_04950 overlap by 38 nucleotides
IAI46_04930: IAI46_04930 - (2E,6E)-farnesyl diphosphate synthase, at 1,062,529 to 1,063,449
_04930
IAI46_04935: IAI46_04935 - exodeoxyribonuclease VII small subunit, at 1,063,455 to 1,063,718
_04935
IAI46_04940: IAI46_04940 - tRNA 4-thiouridine(8) synthase ThiI, at 1,063,951 to 1,065,399
_04940
IAI46_04945: IAI46_04945 - protein deglycase YajL, at 1,065,433 to 1,066,023
_04945
IAI46_04950: IAI46_04950 - 2-dehydropantoate 2-reductase, at 1,065,986 to 1,066,897
_04950
Position (kb)
1063
1064
1065
1066 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1063.689 kb on - strand, within IAI46_04935 at 1063.731 kb on - strand at 1063.768 kb on - strand at 1063.831 kb on + strand at 1063.831 kb on + strand at 1063.832 kb on - strand at 1063.837 kb on + strand at 1063.837 kb on + strand at 1063.837 kb on + strand at 1063.837 kb on + strand at 1063.838 kb on - strand at 1063.838 kb on - strand at 1063.838 kb on - strand at 1063.838 kb on - strand at 1063.839 kb on + strand at 1063.839 kb on + strand at 1063.839 kb on + strand at 1063.839 kb on + strand at 1063.839 kb on + strand at 1063.840 kb on - strand at 1063.840 kb on - strand at 1063.840 kb on - strand at 1063.865 kb on - strand at 1063.902 kb on + strand at 1063.904 kb on + strand at 1063.904 kb on + strand at 1063.904 kb on + strand at 1063.904 kb on + strand at 1063.905 kb on - strand at 1063.905 kb on - strand at 1063.905 kb on - strand at 1063.905 kb on - strand at 1063.911 kb on + strand at 1063.911 kb on + strand at 1063.928 kb on + strand at 1063.940 kb on + strand at 1064.037 kb on + strand at 1064.059 kb on + strand at 1064.060 kb on - strand at 1064.098 kb on - strand, within IAI46_04940 at 1064.202 kb on + strand, within IAI46_04940 at 1064.243 kb on - strand, within IAI46_04940 at 1064.329 kb on + strand, within IAI46_04940 at 1064.330 kb on - strand, within IAI46_04940 at 1064.401 kb on - strand, within IAI46_04940 at 1064.533 kb on + strand, within IAI46_04940 at 1064.533 kb on + strand, within IAI46_04940 at 1064.533 kb on + strand, within IAI46_04940 at 1064.534 kb on - strand, within IAI46_04940 at 1064.535 kb on + strand, within IAI46_04940 at 1064.535 kb on + strand, within IAI46_04940 at 1064.535 kb on + strand, within IAI46_04940 at 1064.536 kb on - strand, within IAI46_04940 at 1064.536 kb on - strand, within IAI46_04940 at 1064.566 kb on + strand, within IAI46_04940 at 1064.566 kb on + strand, within IAI46_04940 at 1064.567 kb on - strand, within IAI46_04940 at 1064.626 kb on + strand, within IAI46_04940 at 1064.743 kb on - strand, within IAI46_04940 at 1064.774 kb on + strand, within IAI46_04940 at 1064.774 kb on + strand, within IAI46_04940 at 1064.774 kb on + strand, within IAI46_04940 at 1064.774 kb on + strand, within IAI46_04940 at 1064.774 kb on + strand, within IAI46_04940 at 1064.775 kb on - strand, within IAI46_04940 at 1064.847 kb on + strand, within IAI46_04940 at 1064.967 kb on + strand, within IAI46_04940 at 1064.967 kb on + strand, within IAI46_04940 at 1064.968 kb on - strand, within IAI46_04940 at 1065.196 kb on - strand, within IAI46_04940 at 1065.260 kb on - strand at 1065.261 kb on + strand at 1065.304 kb on + strand at 1065.304 kb on + strand at 1065.305 kb on - strand at 1065.314 kb on - strand at 1065.353 kb on - strand at 1065.371 kb on - strand at 1065.371 kb on - strand at 1065.511 kb on + strand, within IAI46_04945 at 1065.574 kb on - strand, within IAI46_04945 at 1065.588 kb on - strand, within IAI46_04945 at 1065.588 kb on - strand, within IAI46_04945 at 1065.590 kb on - strand, within IAI46_04945 at 1065.654 kb on + strand, within IAI46_04945 at 1065.655 kb on - strand, within IAI46_04945 at 1065.655 kb on - strand, within IAI46_04945 at 1065.760 kb on + strand, within IAI46_04945 at 1065.761 kb on - strand, within IAI46_04945 at 1065.841 kb on - strand, within IAI46_04945 at 1065.891 kb on + strand, within IAI46_04945 at 1065.891 kb on + strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.924 kb on + strand, within IAI46_04945 at 1065.959 kb on - strand, within IAI46_04945 at 1065.988 kb on - strand at 1066.024 kb on - strand at 1066.024 kb on - strand at 1066.110 kb on + strand, within IAI46_04950 at 1066.123 kb on - strand, within IAI46_04950 at 1066.123 kb on - strand, within IAI46_04950 at 1066.209 kb on + strand, within IAI46_04950 at 1066.209 kb on + strand, within IAI46_04950 at 1066.210 kb on - strand, within IAI46_04950 at 1066.210 kb on - strand, within IAI46_04950 at 1066.234 kb on + strand, within IAI46_04950 at 1066.234 kb on + strand, within IAI46_04950 at 1066.234 kb on + strand, within IAI46_04950 at 1066.235 kb on - strand, within IAI46_04950 at 1066.235 kb on - strand, within IAI46_04950 at 1066.235 kb on - strand, within IAI46_04950 at 1066.239 kb on + strand, within IAI46_04950 at 1066.240 kb on - strand, within IAI46_04950 at 1066.327 kb on + strand, within IAI46_04950 at 1066.327 kb on + strand, within IAI46_04950 at 1066.329 kb on + strand, within IAI46_04950 at 1066.329 kb on + strand, within IAI46_04950 at 1066.330 kb on - strand, within IAI46_04950 at 1066.330 kb on - strand, within IAI46_04950 at 1066.330 kb on - strand, within IAI46_04950 at 1066.348 kb on + strand, within IAI46_04950 at 1066.349 kb on - strand, within IAI46_04950
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, anaerobic remove 1,063,689 - IAI46_04935 0.89 -2.1 1,063,731 - +0.4 1,063,768 - -0.7 1,063,831 + -0.9 1,063,831 + -0.2 1,063,832 - -0.7 1,063,837 + -0.0 1,063,837 + -0.3 1,063,837 + -0.8 1,063,837 + +1.1 1,063,838 - +1.0 1,063,838 - -0.2 1,063,838 - +0.4 1,063,838 - -1.3 1,063,839 + +0.4 1,063,839 + -0.9 1,063,839 + +1.4 1,063,839 + +1.2 1,063,839 + +0.3 1,063,840 - +0.2 1,063,840 - -0.7 1,063,840 - +0.7 1,063,865 - -0.1 1,063,902 + +0.1 1,063,904 + -0.2 1,063,904 + +0.2 1,063,904 + +0.8 1,063,904 + +1.0 1,063,905 - -0.0 1,063,905 - +0.1 1,063,905 - -0.5 1,063,905 - +0.7 1,063,911 + +0.7 1,063,911 + -1.3 1,063,928 + -0.4 1,063,940 + -1.2 1,064,037 + -0.8 1,064,059 + +1.0 1,064,060 - -0.8 1,064,098 - IAI46_04940 0.10 +0.1 1,064,202 + IAI46_04940 0.17 -0.5 1,064,243 - IAI46_04940 0.20 +0.8 1,064,329 + IAI46_04940 0.26 +0.4 1,064,330 - IAI46_04940 0.26 -0.1 1,064,401 - IAI46_04940 0.31 +0.5 1,064,533 + IAI46_04940 0.40 +1.6 1,064,533 + IAI46_04940 0.40 +0.5 1,064,533 + IAI46_04940 0.40 +1.0 1,064,534 - IAI46_04940 0.40 -1.1 1,064,535 + IAI46_04940 0.40 +0.4 1,064,535 + IAI46_04940 0.40 +0.7 1,064,535 + IAI46_04940 0.40 -0.0 1,064,536 - IAI46_04940 0.40 -0.7 1,064,536 - IAI46_04940 0.40 +1.4 1,064,566 + IAI46_04940 0.42 +0.7 1,064,566 + IAI46_04940 0.42 +0.0 1,064,567 - IAI46_04940 0.43 -0.5 1,064,626 + IAI46_04940 0.47 -0.1 1,064,743 - IAI46_04940 0.55 +0.6 1,064,774 + IAI46_04940 0.57 -0.6 1,064,774 + IAI46_04940 0.57 -0.4 1,064,774 + IAI46_04940 0.57 -0.1 1,064,774 + IAI46_04940 0.57 +0.1 1,064,774 + IAI46_04940 0.57 +0.5 1,064,775 - IAI46_04940 0.57 -0.1 1,064,847 + IAI46_04940 0.62 -1.0 1,064,967 + IAI46_04940 0.70 -0.2 1,064,967 + IAI46_04940 0.70 -0.2 1,064,968 - IAI46_04940 0.70 -0.5 1,065,196 - IAI46_04940 0.86 -0.4 1,065,260 - +0.7 1,065,261 + -0.5 1,065,304 + +0.0 1,065,304 + +0.6 1,065,305 - -0.1 1,065,314 - +0.4 1,065,353 - +0.8 1,065,371 - -0.6 1,065,371 - +0.1 1,065,511 + IAI46_04945 0.13 +1.5 1,065,574 - IAI46_04945 0.24 -0.2 1,065,588 - IAI46_04945 0.26 +1.0 1,065,588 - IAI46_04945 0.26 +0.3 1,065,590 - IAI46_04945 0.27 +0.9 1,065,654 + IAI46_04945 0.37 +0.5 1,065,655 - IAI46_04945 0.38 -0.6 1,065,655 - IAI46_04945 0.38 +0.5 1,065,760 + IAI46_04945 0.55 +0.2 1,065,761 - IAI46_04945 0.55 -0.5 1,065,841 - IAI46_04945 0.69 +0.6 1,065,891 + IAI46_04945 0.77 +0.3 1,065,891 + IAI46_04945 0.77 -0.8 1,065,892 - IAI46_04945 0.78 -0.7 1,065,892 - IAI46_04945 0.78 +2.0 1,065,892 - IAI46_04945 0.78 -0.7 1,065,892 - IAI46_04945 0.78 +0.8 1,065,892 - IAI46_04945 0.78 +1.0 1,065,892 - IAI46_04945 0.78 +0.6 1,065,892 - IAI46_04945 0.78 -0.2 1,065,924 + IAI46_04945 0.83 -1.3 1,065,959 - IAI46_04945 0.89 +0.8 1,065,988 - +0.2 1,066,024 - -0.6 1,066,024 - +3.2 1,066,110 + IAI46_04950 0.14 -0.3 1,066,123 - IAI46_04950 0.15 +0.7 1,066,123 - IAI46_04950 0.15 +1.3 1,066,209 + IAI46_04950 0.24 -0.4 1,066,209 + IAI46_04950 0.24 -0.0 1,066,210 - IAI46_04950 0.25 +0.3 1,066,210 - IAI46_04950 0.25 -0.3 1,066,234 + IAI46_04950 0.27 +0.1 1,066,234 + IAI46_04950 0.27 +0.5 1,066,234 + IAI46_04950 0.27 -2.3 1,066,235 - IAI46_04950 0.27 -0.0 1,066,235 - IAI46_04950 0.27 +0.2 1,066,235 - IAI46_04950 0.27 +0.2 1,066,239 + IAI46_04950 0.28 -0.0 1,066,240 - IAI46_04950 0.28 -0.4 1,066,327 + IAI46_04950 0.37 +0.1 1,066,327 + IAI46_04950 0.37 -0.9 1,066,329 + IAI46_04950 0.38 +0.3 1,066,329 + IAI46_04950 0.38 +0.8 1,066,330 - IAI46_04950 0.38 -0.3 1,066,330 - IAI46_04950 0.38 +0.2 1,066,330 - IAI46_04950 0.38 -0.2 1,066,348 + IAI46_04950 0.40 +0.9 1,066,349 - IAI46_04950 0.40 +0.8
Or see this region's nucleotide sequence