Experiment: m.b. Polymyxin B sulfate 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0356 and Echvi_0357 are separated by 53 nucleotides Echvi_0357 and Echvi_0358 are separated by 387 nucleotides Echvi_0358 and Echvi_0359 overlap by 8 nucleotides Echvi_0359 and Echvi_0360 are separated by 24 nucleotides
Echvi_0356: Echvi_0356 - Predicted metal-dependent RNase, consists of a metallo-beta-lactamase domain and an RNA-binding KH domain, at 383,672 to 385,072
_0356
Echvi_0357: Echvi_0357 - hypothetical protein, at 385,126 to 385,608
_0357
Echvi_0358: Echvi_0358 - RNA polymerase sigma factor, sigma-70 family, at 385,996 to 386,565
_0358
Echvi_0359: Echvi_0359 - hypothetical protein, at 386,558 to 387,343
_0359
Echvi_0360: Echvi_0360 - hypothetical protein, at 387,368 to 388,405
_0360
Position (kb) 385
386
387 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 385.021 kb on + strand at 385.021 kb on + strand at 385.190 kb on + strand, within Echvi_0357 at 385.191 kb on - strand, within Echvi_0357 at 385.356 kb on + strand, within Echvi_0357 at 385.357 kb on - strand, within Echvi_0357 at 385.516 kb on + strand, within Echvi_0357 at 385.516 kb on + strand, within Echvi_0357 at 385.517 kb on - strand, within Echvi_0357 at 385.517 kb on - strand, within Echvi_0357 at 385.517 kb on - strand, within Echvi_0357 at 385.517 kb on - strand, within Echvi_0357 at 385.517 kb on - strand, within Echvi_0357 at 385.531 kb on + strand, within Echvi_0357 at 385.533 kb on + strand, within Echvi_0357 at 385.533 kb on + strand, within Echvi_0357 at 385.592 kb on - strand at 385.603 kb on + strand at 385.614 kb on + strand at 385.627 kb on - strand at 385.657 kb on + strand at 385.660 kb on + strand at 385.660 kb on + strand at 385.660 kb on + strand at 385.661 kb on - strand at 385.661 kb on - strand at 385.688 kb on - strand at 385.691 kb on - strand at 385.724 kb on + strand at 385.768 kb on - strand at 385.831 kb on + strand at 385.832 kb on - strand at 385.900 kb on + strand at 385.900 kb on + strand at 385.900 kb on + strand at 385.901 kb on - strand at 385.901 kb on - strand at 385.904 kb on + strand at 385.904 kb on + strand at 385.904 kb on + strand at 385.905 kb on - strand at 385.909 kb on + strand at 385.910 kb on - strand at 386.036 kb on + strand at 386.036 kb on + strand at 386.037 kb on - strand at 386.080 kb on + strand, within Echvi_0358 at 386.081 kb on - strand, within Echvi_0358 at 386.081 kb on - strand, within Echvi_0358 at 386.111 kb on + strand, within Echvi_0358 at 386.111 kb on + strand, within Echvi_0358 at 386.111 kb on + strand, within Echvi_0358 at 386.123 kb on - strand, within Echvi_0358 at 386.123 kb on - strand, within Echvi_0358 at 386.125 kb on - strand, within Echvi_0358 at 386.197 kb on + strand, within Echvi_0358 at 386.294 kb on + strand, within Echvi_0358 at 386.333 kb on - strand, within Echvi_0358 at 386.417 kb on - strand, within Echvi_0358 at 386.417 kb on - strand, within Echvi_0358 at 386.417 kb on - strand, within Echvi_0358 at 386.432 kb on - strand, within Echvi_0358 at 386.513 kb on + strand at 386.514 kb on - strand at 386.514 kb on - strand at 386.535 kb on - strand at 386.535 kb on - strand at 386.691 kb on + strand, within Echvi_0359 at 386.691 kb on + strand, within Echvi_0359 at 386.734 kb on + strand, within Echvi_0359 at 387.264 kb on + strand, within Echvi_0359
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 1 mg/ml remove 385,021 + +0.2 385,021 + +0.6 385,190 + Echvi_0357 0.13 +1.1 385,191 - Echvi_0357 0.13 +0.2 385,356 + Echvi_0357 0.48 +0.0 385,357 - Echvi_0357 0.48 +0.1 385,516 + Echvi_0357 0.81 -0.3 385,516 + Echvi_0357 0.81 -0.2 385,517 - Echvi_0357 0.81 -0.0 385,517 - Echvi_0357 0.81 +1.1 385,517 - Echvi_0357 0.81 -0.8 385,517 - Echvi_0357 0.81 -0.2 385,517 - Echvi_0357 0.81 +1.2 385,531 + Echvi_0357 0.84 -0.9 385,533 + Echvi_0357 0.84 +1.0 385,533 + Echvi_0357 0.84 +0.3 385,592 - +1.5 385,603 + +1.8 385,614 + +0.7 385,627 - +0.8 385,657 + +0.1 385,660 + +0.4 385,660 + -2.8 385,660 + -0.6 385,661 - +0.4 385,661 - +0.6 385,688 - +0.2 385,691 - -0.7 385,724 + -0.7 385,768 - +0.6 385,831 + -1.1 385,832 - +1.0 385,900 + -3.1 385,900 + +0.0 385,900 + -2.6 385,901 - +2.2 385,901 - +1.7 385,904 + +2.6 385,904 + -1.2 385,904 + -1.3 385,905 - +2.3 385,909 + +0.3 385,910 - +2.4 386,036 + -1.9 386,036 + -1.8 386,037 - -1.2 386,080 + Echvi_0358 0.15 -3.2 386,081 - Echvi_0358 0.15 -2.0 386,081 - Echvi_0358 0.15 -1.2 386,111 + Echvi_0358 0.20 -1.5 386,111 + Echvi_0358 0.20 +0.0 386,111 + Echvi_0358 0.20 +0.6 386,123 - Echvi_0358 0.22 -2.9 386,123 - Echvi_0358 0.22 -1.6 386,125 - Echvi_0358 0.23 -3.5 386,197 + Echvi_0358 0.35 -2.2 386,294 + Echvi_0358 0.52 -3.0 386,333 - Echvi_0358 0.59 +1.8 386,417 - Echvi_0358 0.74 +1.6 386,417 - Echvi_0358 0.74 -1.6 386,417 - Echvi_0358 0.74 -3.1 386,432 - Echvi_0358 0.76 -1.1 386,513 + -3.3 386,514 - -1.4 386,514 - -2.5 386,535 - -1.7 386,535 - -2.9 386,691 + Echvi_0359 0.17 -4.3 386,691 + Echvi_0359 0.17 -1.1 386,734 + Echvi_0359 0.22 +0.4 387,264 + Echvi_0359 0.90 +0.3
Or see this region's nucleotide sequence