Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4251 and EX28DRAFT_4252 are separated by 75 nucleotides EX28DRAFT_4252 and EX28DRAFT_4253 overlap by 1 nucleotides EX28DRAFT_4253 and EX28DRAFT_4254 are separated by 18 nucleotides EX28DRAFT_4254 and EX28DRAFT_4255 are separated by 65 nucleotides EX28DRAFT_4255 and EX28DRAFT_4256 are separated by 0 nucleotides
EX28DRAFT_4251: EX28DRAFT_4251 - Protein of unknown function (DUF2589), at 58,625 to 59,182
_4251
EX28DRAFT_4252: EX28DRAFT_4252 - Protein of unknown function (DUF2589), at 59,258 to 59,860
_4252
EX28DRAFT_4253: EX28DRAFT_4253 - Phage-related lysozyme (muraminidase), at 59,860 to 60,309
_4253
EX28DRAFT_4254: EX28DRAFT_4254 - ribonuclease, at 60,328 to 60,810
_4254
EX28DRAFT_4255: EX28DRAFT_4255 - hypothetical protein, at 60,876 to 61,283
_4255
EX28DRAFT_4256: EX28DRAFT_4256 - hypothetical protein, at 61,284 to 61,574
_4256
Position (kb) 59
60
61 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 59.066 kb on - strand, within EX28DRAFT_4251 at 59.089 kb on + strand, within EX28DRAFT_4251 at 59.098 kb on + strand, within EX28DRAFT_4251 at 59.141 kb on + strand at 59.141 kb on + strand at 59.141 kb on + strand at 59.142 kb on - strand at 59.200 kb on + strand at 59.200 kb on + strand at 59.200 kb on + strand at 59.201 kb on - strand at 59.408 kb on - strand, within EX28DRAFT_4252 at 59.408 kb on - strand, within EX28DRAFT_4252 at 59.426 kb on + strand, within EX28DRAFT_4252 at 59.465 kb on + strand, within EX28DRAFT_4252 at 59.465 kb on + strand, within EX28DRAFT_4252 at 59.467 kb on + strand, within EX28DRAFT_4252 at 59.467 kb on + strand, within EX28DRAFT_4252 at 59.467 kb on + strand, within EX28DRAFT_4252 at 59.467 kb on + strand, within EX28DRAFT_4252 at 59.468 kb on - strand, within EX28DRAFT_4252 at 59.814 kb on - strand at 59.880 kb on + strand at 59.881 kb on - strand at 60.238 kb on - strand, within EX28DRAFT_4253 at 60.296 kb on + strand at 60.304 kb on - strand at 60.307 kb on + strand at 60.426 kb on - strand, within EX28DRAFT_4254 at 60.426 kb on - strand, within EX28DRAFT_4254 at 60.460 kb on - strand, within EX28DRAFT_4254 at 60.508 kb on - strand, within EX28DRAFT_4254 at 60.539 kb on - strand, within EX28DRAFT_4254 at 60.540 kb on + strand, within EX28DRAFT_4254 at 60.541 kb on - strand, within EX28DRAFT_4254 at 60.643 kb on + strand, within EX28DRAFT_4254 at 60.733 kb on + strand, within EX28DRAFT_4254 at 60.775 kb on + strand at 60.776 kb on - strand at 60.776 kb on - strand at 60.776 kb on - strand at 60.776 kb on - strand at 60.783 kb on + strand at 60.784 kb on - strand at 60.808 kb on + strand at 60.809 kb on - strand at 60.809 kb on - strand at 60.830 kb on + strand at 60.830 kb on + strand at 60.875 kb on + strand at 60.875 kb on + strand at 60.876 kb on - strand at 60.876 kb on - strand at 60.889 kb on - strand at 60.998 kb on + strand, within EX28DRAFT_4255 at 60.999 kb on - strand, within EX28DRAFT_4255 at 60.999 kb on - strand, within EX28DRAFT_4255 at 61.010 kb on + strand, within EX28DRAFT_4255 at 61.079 kb on - strand, within EX28DRAFT_4255 at 61.119 kb on - strand, within EX28DRAFT_4255 at 61.136 kb on + strand, within EX28DRAFT_4255 at 61.137 kb on - strand, within EX28DRAFT_4255 at 61.295 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 59,066 - EX28DRAFT_4251 0.79 -2.1 59,089 + EX28DRAFT_4251 0.83 -1.1 59,098 + EX28DRAFT_4251 0.85 -0.0 59,141 + +0.6 59,141 + +0.1 59,141 + -0.5 59,142 - -0.3 59,200 + +0.1 59,200 + -0.2 59,200 + +0.4 59,201 - +0.7 59,408 - EX28DRAFT_4252 0.25 -0.4 59,408 - EX28DRAFT_4252 0.25 +0.0 59,426 + EX28DRAFT_4252 0.28 +0.7 59,465 + EX28DRAFT_4252 0.34 +0.7 59,465 + EX28DRAFT_4252 0.34 +0.5 59,467 + EX28DRAFT_4252 0.35 -0.0 59,467 + EX28DRAFT_4252 0.35 +0.3 59,467 + EX28DRAFT_4252 0.35 +0.8 59,467 + EX28DRAFT_4252 0.35 -1.3 59,468 - EX28DRAFT_4252 0.35 -3.6 59,814 - -2.6 59,880 + -1.8 59,881 - +0.0 60,238 - EX28DRAFT_4253 0.84 -1.0 60,296 + -0.1 60,304 - -0.4 60,307 + +0.5 60,426 - EX28DRAFT_4254 0.20 -0.1 60,426 - EX28DRAFT_4254 0.20 -0.0 60,460 - EX28DRAFT_4254 0.27 +0.6 60,508 - EX28DRAFT_4254 0.37 +2.7 60,539 - EX28DRAFT_4254 0.44 -0.1 60,540 + EX28DRAFT_4254 0.44 -1.5 60,541 - EX28DRAFT_4254 0.44 -0.8 60,643 + EX28DRAFT_4254 0.65 +0.1 60,733 + EX28DRAFT_4254 0.84 +0.5 60,775 + +1.5 60,776 - +1.2 60,776 - +0.7 60,776 - -1.6 60,776 - -0.5 60,783 + +1.5 60,784 - -0.2 60,808 + +0.3 60,809 - -0.7 60,809 - +0.1 60,830 + +0.4 60,830 + -0.5 60,875 + +0.4 60,875 + -2.3 60,876 - +0.5 60,876 - +0.0 60,889 - +0.2 60,998 + EX28DRAFT_4255 0.30 +0.2 60,999 - EX28DRAFT_4255 0.30 +1.9 60,999 - EX28DRAFT_4255 0.30 +0.2 61,010 + EX28DRAFT_4255 0.33 +0.9 61,079 - EX28DRAFT_4255 0.50 -1.4 61,119 - EX28DRAFT_4255 0.60 +0.0 61,136 + EX28DRAFT_4255 0.64 -0.2 61,137 - EX28DRAFT_4255 0.64 +0.3 61,295 - -0.3
Or see this region's nucleotide sequence