Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3855 and EX28DRAFT_3856 are separated by 181 nucleotides EX28DRAFT_3856 and EX28DRAFT_3857 are separated by 45 nucleotides EX28DRAFT_3857 and EX28DRAFT_3858 overlap by 4 nucleotides
EX28DRAFT_3855: EX28DRAFT_3855 - L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases, at 65,007 to 66,194
_3855
EX28DRAFT_3856: EX28DRAFT_3856 - Arabinose efflux permease, at 66,376 to 67,716
_3856
EX28DRAFT_3857: EX28DRAFT_3857 - tRNA (cytidine(34)-2'-O)-methyltransferase, at 67,762 to 68,235
_3857
EX28DRAFT_3858: EX28DRAFT_3858 - serine O-acetyltransferase, at 68,232 to 69,053
_3858
Position (kb)
66
67
68 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 65.392 kb on - strand, within EX28DRAFT_3855 at 65.415 kb on - strand, within EX28DRAFT_3855 at 65.664 kb on + strand, within EX28DRAFT_3855 at 65.665 kb on - strand, within EX28DRAFT_3855 at 65.957 kb on + strand, within EX28DRAFT_3855 at 65.958 kb on - strand, within EX28DRAFT_3855 at 65.983 kb on + strand, within EX28DRAFT_3855 at 65.984 kb on - strand, within EX28DRAFT_3855 at 66.000 kb on + strand, within EX28DRAFT_3855 at 66.001 kb on - strand, within EX28DRAFT_3855 at 66.001 kb on - strand, within EX28DRAFT_3855 at 66.120 kb on - strand at 66.142 kb on + strand at 66.274 kb on + strand at 66.323 kb on - strand at 66.323 kb on - strand at 66.375 kb on + strand at 66.376 kb on - strand at 66.382 kb on + strand at 66.508 kb on + strand at 66.829 kb on - strand, within EX28DRAFT_3856 at 66.829 kb on - strand, within EX28DRAFT_3856 at 66.829 kb on - strand, within EX28DRAFT_3856 at 66.835 kb on + strand, within EX28DRAFT_3856 at 66.835 kb on + strand, within EX28DRAFT_3856 at 66.835 kb on + strand, within EX28DRAFT_3856 at 66.835 kb on + strand, within EX28DRAFT_3856 at 66.889 kb on - strand, within EX28DRAFT_3856 at 66.947 kb on + strand, within EX28DRAFT_3856 at 67.175 kb on - strand, within EX28DRAFT_3856 at 67.180 kb on - strand, within EX28DRAFT_3856 at 67.195 kb on + strand, within EX28DRAFT_3856 at 67.196 kb on - strand, within EX28DRAFT_3856 at 67.198 kb on + strand, within EX28DRAFT_3856 at 67.198 kb on + strand, within EX28DRAFT_3856 at 67.198 kb on + strand, within EX28DRAFT_3856 at 67.199 kb on - strand, within EX28DRAFT_3856 at 67.208 kb on + strand, within EX28DRAFT_3856 at 67.408 kb on - strand, within EX28DRAFT_3856 at 67.684 kb on + strand at 67.685 kb on - strand at 67.714 kb on + strand at 67.714 kb on + strand at 67.715 kb on - strand at 67.715 kb on - strand at 67.751 kb on - strand at 67.812 kb on + strand, within EX28DRAFT_3857 at 67.813 kb on - strand, within EX28DRAFT_3857 at 67.872 kb on + strand, within EX28DRAFT_3857 at 67.873 kb on - strand, within EX28DRAFT_3857 at 68.019 kb on + strand, within EX28DRAFT_3857 at 68.047 kb on + strand, within EX28DRAFT_3857 at 68.047 kb on + strand, within EX28DRAFT_3857 at 68.048 kb on - strand, within EX28DRAFT_3857 at 68.186 kb on - strand, within EX28DRAFT_3857 at 68.186 kb on - strand, within EX28DRAFT_3857 at 68.186 kb on - strand, within EX28DRAFT_3857 at 68.233 kb on + strand at 68.259 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 65,392 - EX28DRAFT_3855 0.32 -0.8 65,415 - EX28DRAFT_3855 0.34 -0.7 65,664 + EX28DRAFT_3855 0.55 +2.7 65,665 - EX28DRAFT_3855 0.55 -0.1 65,957 + EX28DRAFT_3855 0.80 +0.6 65,958 - EX28DRAFT_3855 0.80 +0.6 65,983 + EX28DRAFT_3855 0.82 +0.3 65,984 - EX28DRAFT_3855 0.82 -1.1 66,000 + EX28DRAFT_3855 0.84 -0.6 66,001 - EX28DRAFT_3855 0.84 -0.0 66,001 - EX28DRAFT_3855 0.84 +0.2 66,120 - -0.5 66,142 + -0.9 66,274 + -0.0 66,323 - -1.5 66,323 - -0.6 66,375 + -0.2 66,376 - -2.7 66,382 + -0.0 66,508 + +0.2 66,829 - EX28DRAFT_3856 0.34 +0.1 66,829 - EX28DRAFT_3856 0.34 -0.0 66,829 - EX28DRAFT_3856 0.34 +0.5 66,835 + EX28DRAFT_3856 0.34 -0.4 66,835 + EX28DRAFT_3856 0.34 +0.4 66,835 + EX28DRAFT_3856 0.34 +0.4 66,835 + EX28DRAFT_3856 0.34 +0.5 66,889 - EX28DRAFT_3856 0.38 -0.0 66,947 + EX28DRAFT_3856 0.43 +0.7 67,175 - EX28DRAFT_3856 0.60 +0.8 67,180 - EX28DRAFT_3856 0.60 -0.0 67,195 + EX28DRAFT_3856 0.61 +1.0 67,196 - EX28DRAFT_3856 0.61 +0.2 67,198 + EX28DRAFT_3856 0.61 +0.1 67,198 + EX28DRAFT_3856 0.61 +0.1 67,198 + EX28DRAFT_3856 0.61 -0.7 67,199 - EX28DRAFT_3856 0.61 +0.2 67,208 + EX28DRAFT_3856 0.62 -0.7 67,408 - EX28DRAFT_3856 0.77 +1.0 67,684 + +1.1 67,685 - -0.7 67,714 + -0.4 67,714 + -0.1 67,715 - -0.1 67,715 - +0.0 67,751 - +0.2 67,812 + EX28DRAFT_3857 0.11 +0.3 67,813 - EX28DRAFT_3857 0.11 +0.6 67,872 + EX28DRAFT_3857 0.23 +0.6 67,873 - EX28DRAFT_3857 0.23 +1.6 68,019 + EX28DRAFT_3857 0.54 -1.0 68,047 + EX28DRAFT_3857 0.60 -0.7 68,047 + EX28DRAFT_3857 0.60 +0.5 68,048 - EX28DRAFT_3857 0.60 +0.3 68,186 - EX28DRAFT_3857 0.89 -0.1 68,186 - EX28DRAFT_3857 0.89 +0.4 68,186 - EX28DRAFT_3857 0.89 -0.2 68,233 + -0.2 68,259 - +0.2
Or see this region's nucleotide sequence