Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2927 and EX28DRAFT_2928 are separated by 121 nucleotides EX28DRAFT_2928 and EX28DRAFT_2929 are separated by 27 nucleotides EX28DRAFT_2929 and EX28DRAFT_2930 are separated by 10 nucleotides EX28DRAFT_2930 and EX28DRAFT_2931 are separated by 176 nucleotides
EX28DRAFT_2927: EX28DRAFT_2927 - Transcriptional regulators, at 238,539 to 239,009
_2927
EX28DRAFT_2928: EX28DRAFT_2928 - Putative threonine efflux protein, at 239,131 to 239,736
_2928
EX28DRAFT_2929: EX28DRAFT_2929 - Predicted acetyltransferase, at 239,764 to 240,036
_2929
EX28DRAFT_2930: EX28DRAFT_2930 - Uncharacterized conserved protein, at 240,047 to 240,277
_2930
EX28DRAFT_2931: EX28DRAFT_2931 - Signal transduction histidine kinase regulating citrate/malate metabolism, at 240,454 to 242,091
_2931
Position (kb)
239
240
241 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 238.956 kb on + strand, within EX28DRAFT_2927 at 238.974 kb on + strand at 239.136 kb on + strand at 239.136 kb on + strand at 239.137 kb on - strand at 239.172 kb on + strand at 239.306 kb on + strand, within EX28DRAFT_2928 at 239.595 kb on + strand, within EX28DRAFT_2928 at 239.595 kb on + strand, within EX28DRAFT_2928 at 239.605 kb on + strand, within EX28DRAFT_2928 at 239.605 kb on + strand, within EX28DRAFT_2928 at 239.605 kb on + strand, within EX28DRAFT_2928 at 239.606 kb on - strand, within EX28DRAFT_2928 at 239.606 kb on - strand, within EX28DRAFT_2928 at 239.669 kb on + strand, within EX28DRAFT_2928 at 239.711 kb on + strand at 239.717 kb on + strand at 239.717 kb on + strand at 239.718 kb on - strand at 239.784 kb on - strand at 239.784 kb on - strand at 239.797 kb on - strand, within EX28DRAFT_2929 at 239.965 kb on + strand, within EX28DRAFT_2929 at 239.966 kb on - strand, within EX28DRAFT_2929 at 239.966 kb on - strand, within EX28DRAFT_2929 at 240.006 kb on - strand, within EX28DRAFT_2929 at 240.060 kb on + strand at 240.099 kb on - strand, within EX28DRAFT_2930 at 240.154 kb on + strand, within EX28DRAFT_2930 at 240.154 kb on + strand, within EX28DRAFT_2930 at 240.154 kb on + strand, within EX28DRAFT_2930 at 240.184 kb on + strand, within EX28DRAFT_2930 at 240.216 kb on + strand, within EX28DRAFT_2930 at 240.263 kb on + strand at 240.264 kb on - strand at 240.291 kb on + strand at 240.295 kb on + strand at 240.296 kb on - strand at 240.296 kb on - strand at 240.298 kb on + strand at 240.298 kb on + strand at 240.298 kb on + strand at 240.340 kb on + strand at 240.340 kb on + strand at 240.340 kb on + strand at 240.341 kb on - strand at 240.438 kb on - strand at 240.491 kb on + strand at 240.518 kb on + strand at 240.519 kb on - strand at 240.521 kb on + strand at 240.521 kb on + strand at 240.522 kb on - strand at 240.522 kb on - strand at 240.654 kb on + strand, within EX28DRAFT_2931 at 240.654 kb on + strand, within EX28DRAFT_2931 at 240.655 kb on - strand, within EX28DRAFT_2931 at 240.710 kb on - strand, within EX28DRAFT_2931 at 240.772 kb on - strand, within EX28DRAFT_2931 at 240.809 kb on + strand, within EX28DRAFT_2931 at 240.934 kb on + strand, within EX28DRAFT_2931 at 240.934 kb on + strand, within EX28DRAFT_2931 at 240.934 kb on + strand, within EX28DRAFT_2931 at 240.935 kb on - strand, within EX28DRAFT_2931 at 240.935 kb on - strand, within EX28DRAFT_2931 at 240.935 kb on - strand, within EX28DRAFT_2931 at 240.935 kb on - strand, within EX28DRAFT_2931 at 240.936 kb on + strand, within EX28DRAFT_2931 at 240.936 kb on + strand, within EX28DRAFT_2931 at 240.936 kb on + strand, within EX28DRAFT_2931 at 240.937 kb on - strand, within EX28DRAFT_2931 at 240.937 kb on - strand, within EX28DRAFT_2931 at 240.937 kb on - strand, within EX28DRAFT_2931 at 241.026 kb on + strand, within EX28DRAFT_2931
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 238,956 + EX28DRAFT_2927 0.89 +1.8 238,974 + +0.4 239,136 + +0.7 239,136 + +0.5 239,137 - +0.7 239,172 + +0.3 239,306 + EX28DRAFT_2928 0.29 -0.3 239,595 + EX28DRAFT_2928 0.77 -0.1 239,595 + EX28DRAFT_2928 0.77 -0.1 239,605 + EX28DRAFT_2928 0.78 +2.4 239,605 + EX28DRAFT_2928 0.78 +0.1 239,605 + EX28DRAFT_2928 0.78 +0.9 239,606 - EX28DRAFT_2928 0.78 +0.1 239,606 - EX28DRAFT_2928 0.78 -0.1 239,669 + EX28DRAFT_2928 0.89 +0.4 239,711 + +0.0 239,717 + -0.0 239,717 + -2.3 239,718 - -2.4 239,784 - +0.1 239,784 - -0.3 239,797 - EX28DRAFT_2929 0.12 +0.8 239,965 + EX28DRAFT_2929 0.74 +0.4 239,966 - EX28DRAFT_2929 0.74 +1.6 239,966 - EX28DRAFT_2929 0.74 +0.4 240,006 - EX28DRAFT_2929 0.89 +0.3 240,060 + +0.6 240,099 - EX28DRAFT_2930 0.23 -0.3 240,154 + EX28DRAFT_2930 0.46 -0.7 240,154 + EX28DRAFT_2930 0.46 -0.4 240,154 + EX28DRAFT_2930 0.46 -0.3 240,184 + EX28DRAFT_2930 0.59 +1.8 240,216 + EX28DRAFT_2930 0.73 +0.2 240,263 + -0.1 240,264 - -0.7 240,291 + +0.8 240,295 + +2.4 240,296 - -1.4 240,296 - +0.4 240,298 + +0.4 240,298 + +0.7 240,298 + -1.2 240,340 + +0.0 240,340 + +0.3 240,340 + +0.9 240,341 - +0.2 240,438 - +0.4 240,491 + -0.8 240,518 + -0.3 240,519 - -1.3 240,521 + +0.2 240,521 + +0.5 240,522 - -3.9 240,522 - +0.6 240,654 + EX28DRAFT_2931 0.12 +1.2 240,654 + EX28DRAFT_2931 0.12 -0.3 240,655 - EX28DRAFT_2931 0.12 -0.7 240,710 - EX28DRAFT_2931 0.16 +0.0 240,772 - EX28DRAFT_2931 0.19 +0.8 240,809 + EX28DRAFT_2931 0.22 -1.0 240,934 + EX28DRAFT_2931 0.29 -0.4 240,934 + EX28DRAFT_2931 0.29 +1.0 240,934 + EX28DRAFT_2931 0.29 -0.6 240,935 - EX28DRAFT_2931 0.29 -0.1 240,935 - EX28DRAFT_2931 0.29 +0.1 240,935 - EX28DRAFT_2931 0.29 +0.4 240,935 - EX28DRAFT_2931 0.29 -4.4 240,936 + EX28DRAFT_2931 0.29 -0.4 240,936 + EX28DRAFT_2931 0.29 -0.7 240,936 + EX28DRAFT_2931 0.29 -2.0 240,937 - EX28DRAFT_2931 0.29 +0.1 240,937 - EX28DRAFT_2931 0.29 +1.0 240,937 - EX28DRAFT_2931 0.29 +0.0 241,026 + EX28DRAFT_2931 0.35 +2.0
Or see this region's nucleotide sequence