Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2758 and EX28DRAFT_2759 are separated by 243 nucleotides EX28DRAFT_2759 and EX28DRAFT_2760 overlap by 4 nucleotides EX28DRAFT_2760 and EX28DRAFT_2761 overlap by 28 nucleotides EX28DRAFT_2761 and EX28DRAFT_2762 are separated by 9 nucleotides
EX28DRAFT_2758: EX28DRAFT_2758 - Uncharacterized conserved protein, at 65,593 to 65,919
_2758
EX28DRAFT_2759: EX28DRAFT_2759 - ribonucleoside-diphosphate reductase class Ib glutaredoxin subunit (EC 1.17.4.1), at 66,163 to 66,402
_2759
EX28DRAFT_2760: EX28DRAFT_2760 - ribonucleoside-diphosphate reductase 2, operon protein nrdI, at 66,399 to 66,809
_2760
EX28DRAFT_2761: EX28DRAFT_2761 - ribonucleoside-diphosphate reductase, alpha subunit, at 66,782 to 68,926
_2761
EX28DRAFT_2762: EX28DRAFT_2762 - ribonucleoside-diphosphate reductase, class 1b, beta subunit, at 68,936 to 69,895
_2762
Position (kb) 66
67
68
69 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 65.853 kb on - strand, within EX28DRAFT_2758 at 65.856 kb on + strand, within EX28DRAFT_2758 at 65.856 kb on + strand, within EX28DRAFT_2758 at 65.856 kb on + strand, within EX28DRAFT_2758 at 65.857 kb on - strand, within EX28DRAFT_2758 at 65.857 kb on - strand, within EX28DRAFT_2758 at 65.918 kb on - strand at 66.033 kb on - strand at 66.078 kb on - strand at 66.078 kb on - strand at 66.104 kb on - strand at 66.104 kb on - strand at 66.399 kb on - strand at 66.414 kb on + strand at 66.414 kb on + strand at 66.512 kb on - strand, within EX28DRAFT_2760 at 66.522 kb on + strand, within EX28DRAFT_2760 at 66.543 kb on + strand, within EX28DRAFT_2760 at 66.764 kb on + strand, within EX28DRAFT_2760 at 66.858 kb on - strand at 66.992 kb on + strand at 66.993 kb on - strand at 67.106 kb on + strand, within EX28DRAFT_2761 at 67.107 kb on - strand, within EX28DRAFT_2761 at 67.115 kb on + strand, within EX28DRAFT_2761 at 67.116 kb on - strand, within EX28DRAFT_2761 at 67.118 kb on - strand, within EX28DRAFT_2761 at 67.146 kb on - strand, within EX28DRAFT_2761 at 67.150 kb on - strand, within EX28DRAFT_2761 at 67.327 kb on + strand, within EX28DRAFT_2761 at 67.339 kb on + strand, within EX28DRAFT_2761 at 67.340 kb on - strand, within EX28DRAFT_2761 at 67.340 kb on - strand, within EX28DRAFT_2761 at 67.512 kb on - strand, within EX28DRAFT_2761 at 67.673 kb on - strand, within EX28DRAFT_2761 at 67.673 kb on - strand, within EX28DRAFT_2761 at 67.688 kb on - strand, within EX28DRAFT_2761 at 67.729 kb on + strand, within EX28DRAFT_2761 at 67.874 kb on + strand, within EX28DRAFT_2761 at 67.997 kb on + strand, within EX28DRAFT_2761 at 68.017 kb on + strand, within EX28DRAFT_2761 at 68.149 kb on + strand, within EX28DRAFT_2761 at 68.271 kb on - strand, within EX28DRAFT_2761 at 68.516 kb on + strand, within EX28DRAFT_2761 at 68.517 kb on - strand, within EX28DRAFT_2761 at 68.517 kb on - strand, within EX28DRAFT_2761 at 68.561 kb on + strand, within EX28DRAFT_2761 at 68.646 kb on - strand, within EX28DRAFT_2761 at 68.696 kb on + strand, within EX28DRAFT_2761 at 68.697 kb on - strand, within EX28DRAFT_2761 at 68.697 kb on - strand, within EX28DRAFT_2761 at 68.697 kb on - strand, within EX28DRAFT_2761 at 68.826 kb on - strand at 68.855 kb on + strand at 68.856 kb on - strand at 68.856 kb on - strand at 69.248 kb on + strand, within EX28DRAFT_2762 at 69.357 kb on - strand, within EX28DRAFT_2762 at 69.446 kb on + strand, within EX28DRAFT_2762 at 69.447 kb on - strand, within EX28DRAFT_2762 at 69.527 kb on + strand, within EX28DRAFT_2762 at 69.528 kb on - strand, within EX28DRAFT_2762 at 69.538 kb on + strand, within EX28DRAFT_2762 at 69.539 kb on - strand, within EX28DRAFT_2762 at 69.589 kb on + strand, within EX28DRAFT_2762 at 69.641 kb on + strand, within EX28DRAFT_2762 at 69.657 kb on - strand, within EX28DRAFT_2762 at 69.735 kb on - strand, within EX28DRAFT_2762 at 69.779 kb on - strand, within EX28DRAFT_2762 at 69.923 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 65,853 - EX28DRAFT_2758 0.80 -0.4 65,856 + EX28DRAFT_2758 0.80 +1.0 65,856 + EX28DRAFT_2758 0.80 -1.0 65,856 + EX28DRAFT_2758 0.80 -1.2 65,857 - EX28DRAFT_2758 0.81 +1.7 65,857 - EX28DRAFT_2758 0.81 +0.5 65,918 - -1.0 66,033 - -0.9 66,078 - +0.9 66,078 - +1.4 66,104 - -0.2 66,104 - -2.1 66,399 - +1.0 66,414 + +0.7 66,414 + +0.2 66,512 - EX28DRAFT_2760 0.27 +0.6 66,522 + EX28DRAFT_2760 0.30 -1.4 66,543 + EX28DRAFT_2760 0.35 +1.0 66,764 + EX28DRAFT_2760 0.89 +0.5 66,858 - -2.5 66,992 + +0.0 66,993 - -0.0 67,106 + EX28DRAFT_2761 0.15 +0.1 67,107 - EX28DRAFT_2761 0.15 +0.6 67,115 + EX28DRAFT_2761 0.16 +0.6 67,116 - EX28DRAFT_2761 0.16 -3.9 67,118 - EX28DRAFT_2761 0.16 -3.2 67,146 - EX28DRAFT_2761 0.17 -0.3 67,150 - EX28DRAFT_2761 0.17 -2.0 67,327 + EX28DRAFT_2761 0.25 +0.2 67,339 + EX28DRAFT_2761 0.26 +0.6 67,340 - EX28DRAFT_2761 0.26 +0.5 67,340 - EX28DRAFT_2761 0.26 +0.0 67,512 - EX28DRAFT_2761 0.34 +0.5 67,673 - EX28DRAFT_2761 0.42 +0.5 67,673 - EX28DRAFT_2761 0.42 -0.7 67,688 - EX28DRAFT_2761 0.42 +2.1 67,729 + EX28DRAFT_2761 0.44 -0.0 67,874 + EX28DRAFT_2761 0.51 -0.1 67,997 + EX28DRAFT_2761 0.57 -0.5 68,017 + EX28DRAFT_2761 0.58 +1.0 68,149 + EX28DRAFT_2761 0.64 +0.9 68,271 - EX28DRAFT_2761 0.69 -0.3 68,516 + EX28DRAFT_2761 0.81 -1.3 68,517 - EX28DRAFT_2761 0.81 +0.2 68,517 - EX28DRAFT_2761 0.81 +0.9 68,561 + EX28DRAFT_2761 0.83 -0.2 68,646 - EX28DRAFT_2761 0.87 +0.5 68,696 + EX28DRAFT_2761 0.89 -0.7 68,697 - EX28DRAFT_2761 0.89 +0.9 68,697 - EX28DRAFT_2761 0.89 +0.5 68,697 - EX28DRAFT_2761 0.89 -0.4 68,826 - -0.0 68,855 + +1.3 68,856 - -0.1 68,856 - -5.0 69,248 + EX28DRAFT_2762 0.33 -0.2 69,357 - EX28DRAFT_2762 0.44 -2.0 69,446 + EX28DRAFT_2762 0.53 -0.2 69,447 - EX28DRAFT_2762 0.53 -0.4 69,527 + EX28DRAFT_2762 0.62 +0.7 69,528 - EX28DRAFT_2762 0.62 +0.7 69,538 + EX28DRAFT_2762 0.63 +1.2 69,539 - EX28DRAFT_2762 0.63 -0.8 69,589 + EX28DRAFT_2762 0.68 +0.8 69,641 + EX28DRAFT_2762 0.73 -0.6 69,657 - EX28DRAFT_2762 0.75 +1.4 69,735 - EX28DRAFT_2762 0.83 +0.1 69,779 - EX28DRAFT_2762 0.88 +1.5 69,923 + -1.2
Or see this region's nucleotide sequence