Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2388 and EX28DRAFT_2389 are separated by 143 nucleotides EX28DRAFT_2389 and EX28DRAFT_2390 are separated by 43 nucleotides EX28DRAFT_2390 and EX28DRAFT_2391 overlap by 4 nucleotides EX28DRAFT_2391 and EX28DRAFT_2392 are separated by 328 nucleotides
EX28DRAFT_2388: EX28DRAFT_2388 - choline dehydrogenase, at 2,413,545 to 2,415,209
_2388
EX28DRAFT_2389: EX28DRAFT_2389 - Secreted and surface protein containing fasciclin-like repeats, at 2,415,353 to 2,415,916
_2389
EX28DRAFT_2390: EX28DRAFT_2390 - RNA polymerase sigma factor, sigma-70 family, at 2,415,960 to 2,416,514
_2390
EX28DRAFT_2391: EX28DRAFT_2391 - Uncharacterized protein conserved in bacteria, at 2,416,511 to 2,417,158
_2391
EX28DRAFT_2392: EX28DRAFT_2392 - Short-chain alcohol dehydrogenase of unknown specificity, at 2,417,487 to 2,418,227
_2392
Position (kb)
2415
2416
2417 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2415.105 kb on - strand at 2415.108 kb on - strand at 2415.174 kb on + strand at 2415.177 kb on + strand at 2415.177 kb on + strand at 2415.177 kb on + strand at 2415.178 kb on - strand at 2415.178 kb on - strand at 2415.265 kb on - strand at 2415.304 kb on + strand at 2415.304 kb on + strand at 2415.304 kb on + strand at 2415.304 kb on + strand at 2415.312 kb on + strand at 2415.313 kb on - strand at 2415.347 kb on + strand at 2415.348 kb on - strand at 2415.550 kb on + strand, within EX28DRAFT_2389 at 2415.652 kb on + strand, within EX28DRAFT_2389 at 2415.671 kb on - strand, within EX28DRAFT_2389 at 2415.680 kb on + strand, within EX28DRAFT_2389 at 2415.680 kb on + strand, within EX28DRAFT_2389 at 2415.681 kb on - strand, within EX28DRAFT_2389 at 2415.701 kb on + strand, within EX28DRAFT_2389 at 2415.702 kb on - strand, within EX28DRAFT_2389 at 2415.702 kb on - strand, within EX28DRAFT_2389 at 2415.702 kb on - strand, within EX28DRAFT_2389 at 2415.854 kb on + strand, within EX28DRAFT_2389 at 2415.855 kb on - strand, within EX28DRAFT_2389 at 2415.855 kb on - strand, within EX28DRAFT_2389 at 2415.855 kb on - strand, within EX28DRAFT_2389 at 2415.910 kb on + strand at 2415.910 kb on + strand at 2415.914 kb on + strand at 2415.915 kb on - strand at 2415.922 kb on + strand at 2415.925 kb on - strand at 2416.159 kb on - strand, within EX28DRAFT_2390 at 2416.199 kb on + strand, within EX28DRAFT_2390 at 2416.200 kb on - strand, within EX28DRAFT_2390 at 2416.217 kb on + strand, within EX28DRAFT_2390 at 2416.218 kb on - strand, within EX28DRAFT_2390 at 2416.270 kb on - strand, within EX28DRAFT_2390 at 2416.554 kb on - strand at 2416.763 kb on + strand, within EX28DRAFT_2391 at 2416.978 kb on + strand, within EX28DRAFT_2391 at 2416.979 kb on - strand, within EX28DRAFT_2391 at 2416.979 kb on - strand, within EX28DRAFT_2391 at 2417.027 kb on - strand, within EX28DRAFT_2391 at 2417.028 kb on + strand, within EX28DRAFT_2391 at 2417.028 kb on + strand, within EX28DRAFT_2391 at 2417.028 kb on + strand, within EX28DRAFT_2391 at 2417.141 kb on + strand at 2417.194 kb on - strand at 2417.261 kb on - strand at 2417.267 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,415,105 - -0.2 2,415,108 - +0.3 2,415,174 + -0.4 2,415,177 + -0.1 2,415,177 + +0.6 2,415,177 + -1.4 2,415,178 - -4.2 2,415,178 - -0.6 2,415,265 - +0.6 2,415,304 + +0.0 2,415,304 + +0.1 2,415,304 + +0.4 2,415,304 + -0.5 2,415,312 + -0.5 2,415,313 - -2.7 2,415,347 + +0.3 2,415,348 - -0.7 2,415,550 + EX28DRAFT_2389 0.35 +0.9 2,415,652 + EX28DRAFT_2389 0.53 -4.9 2,415,671 - EX28DRAFT_2389 0.56 -1.0 2,415,680 + EX28DRAFT_2389 0.58 -0.9 2,415,680 + EX28DRAFT_2389 0.58 -1.2 2,415,681 - EX28DRAFT_2389 0.58 -0.1 2,415,701 + EX28DRAFT_2389 0.62 -0.8 2,415,702 - EX28DRAFT_2389 0.62 +0.0 2,415,702 - EX28DRAFT_2389 0.62 -0.2 2,415,702 - EX28DRAFT_2389 0.62 -1.7 2,415,854 + EX28DRAFT_2389 0.89 -2.2 2,415,855 - EX28DRAFT_2389 0.89 -0.2 2,415,855 - EX28DRAFT_2389 0.89 +0.3 2,415,855 - EX28DRAFT_2389 0.89 -0.5 2,415,910 + +0.0 2,415,910 + +1.2 2,415,914 + +0.3 2,415,915 - -1.6 2,415,922 + -1.2 2,415,925 - +0.3 2,416,159 - EX28DRAFT_2390 0.36 -3.2 2,416,199 + EX28DRAFT_2390 0.43 -1.2 2,416,200 - EX28DRAFT_2390 0.43 -0.5 2,416,217 + EX28DRAFT_2390 0.46 -1.0 2,416,218 - EX28DRAFT_2390 0.46 -0.4 2,416,270 - EX28DRAFT_2390 0.56 -0.6 2,416,554 - +0.7 2,416,763 + EX28DRAFT_2391 0.39 -1.0 2,416,978 + EX28DRAFT_2391 0.72 -1.4 2,416,979 - EX28DRAFT_2391 0.72 +0.6 2,416,979 - EX28DRAFT_2391 0.72 +0.3 2,417,027 - EX28DRAFT_2391 0.80 +0.6 2,417,028 + EX28DRAFT_2391 0.80 -1.1 2,417,028 + EX28DRAFT_2391 0.80 +0.5 2,417,028 + EX28DRAFT_2391 0.80 -0.1 2,417,141 + +1.1 2,417,194 - -0.2 2,417,261 - -1.8 2,417,267 + -0.5
Or see this region's nucleotide sequence