Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2140 and EX28DRAFT_2141 are separated by 4 nucleotides EX28DRAFT_2141 and EX28DRAFT_2142 are separated by 192 nucleotides
EX28DRAFT_2140: EX28DRAFT_2140 - glycyl-radical enzyme activating protein, at 2,160,062 to 2,160,961
_2140
EX28DRAFT_2141: EX28DRAFT_2141 - glycyl radical enzyme, PFL2/glycerol dehydratase family, at 2,160,966 to 2,163,398
_2141
EX28DRAFT_2142: EX28DRAFT_2142 - HAD-superfamily hydrolase, subfamily IIB, at 2,163,591 to 2,164,406
_2142
Position (kb)
2160
2161
2162
2163
2164 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2160.419 kb on + strand, within EX28DRAFT_2140 at 2160.465 kb on - strand, within EX28DRAFT_2140 at 2160.465 kb on - strand, within EX28DRAFT_2140 at 2160.465 kb on - strand, within EX28DRAFT_2140 at 2160.466 kb on + strand, within EX28DRAFT_2140 at 2160.467 kb on - strand, within EX28DRAFT_2140 at 2160.560 kb on + strand, within EX28DRAFT_2140 at 2160.560 kb on + strand, within EX28DRAFT_2140 at 2160.561 kb on - strand, within EX28DRAFT_2140 at 2160.577 kb on - strand, within EX28DRAFT_2140 at 2160.766 kb on + strand, within EX28DRAFT_2140 at 2160.803 kb on - strand, within EX28DRAFT_2140 at 2160.842 kb on + strand, within EX28DRAFT_2140 at 2160.843 kb on - strand, within EX28DRAFT_2140 at 2160.864 kb on - strand, within EX28DRAFT_2140 at 2160.921 kb on - strand at 2161.004 kb on + strand at 2161.005 kb on - strand at 2161.071 kb on + strand at 2161.071 kb on + strand at 2161.083 kb on + strand at 2161.234 kb on - strand, within EX28DRAFT_2141 at 2161.771 kb on - strand, within EX28DRAFT_2141 at 2161.840 kb on - strand, within EX28DRAFT_2141 at 2161.855 kb on - strand, within EX28DRAFT_2141 at 2162.265 kb on + strand, within EX28DRAFT_2141 at 2162.496 kb on + strand, within EX28DRAFT_2141 at 2162.497 kb on - strand, within EX28DRAFT_2141 at 2162.562 kb on - strand, within EX28DRAFT_2141 at 2162.632 kb on - strand, within EX28DRAFT_2141 at 2162.818 kb on - strand, within EX28DRAFT_2141 at 2162.871 kb on + strand, within EX28DRAFT_2141 at 2162.872 kb on - strand, within EX28DRAFT_2141 at 2162.872 kb on - strand, within EX28DRAFT_2141 at 2162.892 kb on + strand, within EX28DRAFT_2141 at 2162.938 kb on - strand, within EX28DRAFT_2141 at 2163.008 kb on + strand, within EX28DRAFT_2141 at 2163.008 kb on + strand, within EX28DRAFT_2141 at 2163.008 kb on + strand, within EX28DRAFT_2141 at 2163.104 kb on + strand, within EX28DRAFT_2141 at 2163.234 kb on - strand at 2163.241 kb on - strand at 2163.325 kb on - strand at 2163.368 kb on + strand at 2163.368 kb on + strand at 2163.390 kb on - strand at 2163.390 kb on - strand at 2163.405 kb on - strand at 2163.555 kb on - strand at 2163.591 kb on - strand at 2163.591 kb on - strand at 2163.735 kb on + strand, within EX28DRAFT_2142 at 2163.735 kb on + strand, within EX28DRAFT_2142 at 2163.735 kb on + strand, within EX28DRAFT_2142 at 2163.979 kb on - strand, within EX28DRAFT_2142 at 2164.032 kb on - strand, within EX28DRAFT_2142 at 2164.032 kb on - strand, within EX28DRAFT_2142
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,160,419 + EX28DRAFT_2140 0.40 -0.2 2,160,465 - EX28DRAFT_2140 0.45 -2.8 2,160,465 - EX28DRAFT_2140 0.45 +0.0 2,160,465 - EX28DRAFT_2140 0.45 -1.3 2,160,466 + EX28DRAFT_2140 0.45 +0.2 2,160,467 - EX28DRAFT_2140 0.45 +0.1 2,160,560 + EX28DRAFT_2140 0.55 +0.4 2,160,560 + EX28DRAFT_2140 0.55 +1.2 2,160,561 - EX28DRAFT_2140 0.55 -0.2 2,160,577 - EX28DRAFT_2140 0.57 -0.3 2,160,766 + EX28DRAFT_2140 0.78 -0.5 2,160,803 - EX28DRAFT_2140 0.82 -1.0 2,160,842 + EX28DRAFT_2140 0.87 +0.1 2,160,843 - EX28DRAFT_2140 0.87 +0.6 2,160,864 - EX28DRAFT_2140 0.89 -0.1 2,160,921 - +0.4 2,161,004 + -1.8 2,161,005 - +0.1 2,161,071 + +0.2 2,161,071 + +0.9 2,161,083 + -1.7 2,161,234 - EX28DRAFT_2141 0.11 -1.0 2,161,771 - EX28DRAFT_2141 0.33 +0.7 2,161,840 - EX28DRAFT_2141 0.36 -0.5 2,161,855 - EX28DRAFT_2141 0.37 -0.6 2,162,265 + EX28DRAFT_2141 0.53 -0.1 2,162,496 + EX28DRAFT_2141 0.63 +1.6 2,162,497 - EX28DRAFT_2141 0.63 -1.8 2,162,562 - EX28DRAFT_2141 0.66 -0.4 2,162,632 - EX28DRAFT_2141 0.68 +0.6 2,162,818 - EX28DRAFT_2141 0.76 +0.4 2,162,871 + EX28DRAFT_2141 0.78 +0.0 2,162,872 - EX28DRAFT_2141 0.78 +0.1 2,162,872 - EX28DRAFT_2141 0.78 +0.3 2,162,892 + EX28DRAFT_2141 0.79 -1.4 2,162,938 - EX28DRAFT_2141 0.81 -0.7 2,163,008 + EX28DRAFT_2141 0.84 +0.4 2,163,008 + EX28DRAFT_2141 0.84 +1.3 2,163,008 + EX28DRAFT_2141 0.84 +0.2 2,163,104 + EX28DRAFT_2141 0.88 -1.0 2,163,234 - +1.1 2,163,241 - -0.2 2,163,325 - +1.0 2,163,368 + -0.7 2,163,368 + +0.3 2,163,390 - -0.3 2,163,390 - -0.4 2,163,405 - +0.8 2,163,555 - -2.3 2,163,591 - +0.4 2,163,591 - -0.6 2,163,735 + EX28DRAFT_2142 0.18 +1.1 2,163,735 + EX28DRAFT_2142 0.18 +1.6 2,163,735 + EX28DRAFT_2142 0.18 -1.0 2,163,979 - EX28DRAFT_2142 0.48 +0.4 2,164,032 - EX28DRAFT_2142 0.54 +0.6 2,164,032 - EX28DRAFT_2142 0.54 +0.7
Or see this region's nucleotide sequence