Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1665 and EX28DRAFT_1666 are separated by 72 nucleotides EX28DRAFT_1666 and EX28DRAFT_1667 are separated by 125 nucleotides EX28DRAFT_1667 and EX28DRAFT_1668 are separated by 92 nucleotides EX28DRAFT_1668 and EX28DRAFT_1669 are separated by 239 nucleotides
EX28DRAFT_1665: EX28DRAFT_1665 - Carbonic anhydrase, at 1,680,166 to 1,680,801
_1665
EX28DRAFT_1666: EX28DRAFT_1666 - hypothetical protein, at 1,680,874 to 1,681,656
_1666
EX28DRAFT_1667: EX28DRAFT_1667 - hypothetical protein, at 1,681,782 to 1,681,922
_1667
EX28DRAFT_1668: EX28DRAFT_1668 - hypothetical protein, at 1,682,015 to 1,682,182
_1668
EX28DRAFT_1669: EX28DRAFT_1669 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 1,682,422 to 1,683,258
_1669
Position (kb)
1681
1682 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1680.817 kb on - strand at 1680.817 kb on - strand at 1680.862 kb on - strand at 1680.875 kb on + strand at 1680.876 kb on - strand at 1680.876 kb on - strand at 1680.876 kb on - strand at 1680.891 kb on + strand at 1680.891 kb on + strand at 1680.892 kb on - strand at 1680.892 kb on - strand at 1680.893 kb on + strand at 1680.894 kb on - strand at 1680.894 kb on - strand at 1680.894 kb on - strand at 1681.043 kb on + strand, within EX28DRAFT_1666 at 1681.053 kb on + strand, within EX28DRAFT_1666 at 1681.054 kb on - strand, within EX28DRAFT_1666 at 1681.109 kb on - strand, within EX28DRAFT_1666 at 1681.313 kb on + strand, within EX28DRAFT_1666 at 1681.314 kb on - strand, within EX28DRAFT_1666 at 1681.314 kb on - strand, within EX28DRAFT_1666 at 1681.314 kb on - strand, within EX28DRAFT_1666 at 1681.316 kb on + strand, within EX28DRAFT_1666 at 1681.317 kb on - strand, within EX28DRAFT_1666 at 1681.317 kb on - strand, within EX28DRAFT_1666 at 1681.518 kb on + strand, within EX28DRAFT_1666 at 1681.575 kb on - strand, within EX28DRAFT_1666 at 1681.622 kb on + strand at 1681.623 kb on - strand at 1681.641 kb on + strand at 1681.651 kb on - strand at 1681.685 kb on + strand at 1681.686 kb on - strand at 1681.686 kb on - strand at 1681.703 kb on + strand at 1681.704 kb on - strand at 1681.704 kb on - strand at 1681.740 kb on + strand at 1681.740 kb on + strand at 1681.740 kb on + strand at 1681.741 kb on - strand at 1681.742 kb on + strand at 1681.742 kb on + strand at 1681.743 kb on - strand at 1681.804 kb on + strand, within EX28DRAFT_1667 at 1681.804 kb on + strand, within EX28DRAFT_1667 at 1681.838 kb on + strand, within EX28DRAFT_1667 at 1681.839 kb on - strand, within EX28DRAFT_1667 at 1681.920 kb on + strand at 1681.923 kb on + strand at 1681.924 kb on - strand at 1681.953 kb on + strand at 1682.029 kb on + strand at 1682.217 kb on + strand at 1682.217 kb on + strand at 1682.217 kb on + strand at 1682.281 kb on + strand at 1682.282 kb on - strand at 1682.398 kb on - strand at 1682.428 kb on - strand at 1682.532 kb on + strand, within EX28DRAFT_1669 at 1682.536 kb on - strand, within EX28DRAFT_1669 at 1682.592 kb on + strand, within EX28DRAFT_1669 at 1682.751 kb on + strand, within EX28DRAFT_1669 at 1682.752 kb on - strand, within EX28DRAFT_1669 at 1682.754 kb on + strand, within EX28DRAFT_1669 at 1682.754 kb on + strand, within EX28DRAFT_1669 at 1682.754 kb on + strand, within EX28DRAFT_1669 at 1682.755 kb on - strand, within EX28DRAFT_1669 at 1682.832 kb on + strand, within EX28DRAFT_1669
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,680,817 - +0.0 1,680,817 - -0.2 1,680,862 - +0.4 1,680,875 + +0.5 1,680,876 - -1.5 1,680,876 - -0.7 1,680,876 - +0.4 1,680,891 + +0.1 1,680,891 + -0.1 1,680,892 - -3.4 1,680,892 - -1.8 1,680,893 + +0.8 1,680,894 - +0.6 1,680,894 - +0.2 1,680,894 - -0.9 1,681,043 + EX28DRAFT_1666 0.22 -0.7 1,681,053 + EX28DRAFT_1666 0.23 +1.0 1,681,054 - EX28DRAFT_1666 0.23 +0.8 1,681,109 - EX28DRAFT_1666 0.30 -1.2 1,681,313 + EX28DRAFT_1666 0.56 -0.6 1,681,314 - EX28DRAFT_1666 0.56 -1.1 1,681,314 - EX28DRAFT_1666 0.56 +0.6 1,681,314 - EX28DRAFT_1666 0.56 +0.9 1,681,316 + EX28DRAFT_1666 0.56 -2.9 1,681,317 - EX28DRAFT_1666 0.57 -0.3 1,681,317 - EX28DRAFT_1666 0.57 -2.2 1,681,518 + EX28DRAFT_1666 0.82 -0.1 1,681,575 - EX28DRAFT_1666 0.90 -0.6 1,681,622 + -0.5 1,681,623 - -0.3 1,681,641 + +0.3 1,681,651 - -1.3 1,681,685 + +0.6 1,681,686 - -0.1 1,681,686 - -3.3 1,681,703 + -0.3 1,681,704 - -0.7 1,681,704 - +0.4 1,681,740 + -0.5 1,681,740 + -0.5 1,681,740 + +0.5 1,681,741 - -1.3 1,681,742 + -3.6 1,681,742 + -0.5 1,681,743 - +0.5 1,681,804 + EX28DRAFT_1667 0.16 +0.1 1,681,804 + EX28DRAFT_1667 0.16 -0.3 1,681,838 + EX28DRAFT_1667 0.40 +0.0 1,681,839 - EX28DRAFT_1667 0.40 -0.9 1,681,920 + -1.3 1,681,923 + -3.7 1,681,924 - -1.1 1,681,953 + -0.6 1,682,029 + -0.3 1,682,217 + -2.1 1,682,217 + +0.5 1,682,217 + +0.2 1,682,281 + +1.6 1,682,282 - +4.1 1,682,398 - -0.6 1,682,428 - -1.0 1,682,532 + EX28DRAFT_1669 0.13 +0.6 1,682,536 - EX28DRAFT_1669 0.14 -0.1 1,682,592 + EX28DRAFT_1669 0.20 +3.1 1,682,751 + EX28DRAFT_1669 0.39 -0.8 1,682,752 - EX28DRAFT_1669 0.39 -1.1 1,682,754 + EX28DRAFT_1669 0.40 -1.4 1,682,754 + EX28DRAFT_1669 0.40 +1.8 1,682,754 + EX28DRAFT_1669 0.40 +0.5 1,682,755 - EX28DRAFT_1669 0.40 +1.9 1,682,832 + EX28DRAFT_1669 0.49 +0.9
Or see this region's nucleotide sequence