Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1650 and EX28DRAFT_1651 are separated by 44 nucleotides EX28DRAFT_1651 and EX28DRAFT_1652 are separated by 338 nucleotides EX28DRAFT_1652 and EX28DRAFT_1653 are separated by 122 nucleotides EX28DRAFT_1653 and EX28DRAFT_1654 are separated by 38 nucleotides
EX28DRAFT_1650: EX28DRAFT_1650 - ribonuclease T, at 1,666,510 to 1,667,160
_1650
EX28DRAFT_1651: EX28DRAFT_1651 - monothiol glutaredoxin, Grx4 family, at 1,667,205 to 1,667,552
_1651
EX28DRAFT_1652: EX28DRAFT_1652 - Cell wall-associated hydrolases (invasion-associated proteins), at 1,667,891 to 1,668,664
_1652
EX28DRAFT_1653: EX28DRAFT_1653 - Superoxide dismutase, at 1,668,787 to 1,669,368
_1653
EX28DRAFT_1654: EX28DRAFT_1654 - Arabinose efflux permease, at 1,669,407 to 1,670,573
_1654
Position (kb)
1667
1668
1669 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1667.004 kb on + strand, within EX28DRAFT_1650 at 1667.191 kb on - strand at 1667.206 kb on + strand at 1667.282 kb on - strand, within EX28DRAFT_1651 at 1667.360 kb on - strand, within EX28DRAFT_1651 at 1667.360 kb on - strand, within EX28DRAFT_1651 at 1667.400 kb on - strand, within EX28DRAFT_1651 at 1667.492 kb on + strand, within EX28DRAFT_1651 at 1667.493 kb on - strand, within EX28DRAFT_1651 at 1667.494 kb on + strand, within EX28DRAFT_1651 at 1667.494 kb on + strand, within EX28DRAFT_1651 at 1667.495 kb on - strand, within EX28DRAFT_1651 at 1667.495 kb on - strand, within EX28DRAFT_1651 at 1667.567 kb on - strand at 1667.567 kb on - strand at 1667.567 kb on - strand at 1667.567 kb on - strand at 1667.641 kb on + strand at 1667.641 kb on + strand at 1667.643 kb on + strand at 1667.644 kb on - strand at 1667.650 kb on + strand at 1667.688 kb on + strand at 1667.780 kb on + strand at 1667.781 kb on - strand at 1667.781 kb on - strand at 1667.781 kb on - strand at 1667.781 kb on - strand at 1667.796 kb on - strand at 1667.798 kb on + strand at 1667.814 kb on + strand at 1667.821 kb on + strand at 1667.822 kb on - strand at 1667.822 kb on - strand at 1667.839 kb on + strand at 1667.839 kb on + strand at 1667.839 kb on + strand at 1667.931 kb on + strand at 1667.938 kb on + strand at 1668.247 kb on + strand, within EX28DRAFT_1652 at 1668.314 kb on + strand, within EX28DRAFT_1652 at 1668.371 kb on + strand, within EX28DRAFT_1652 at 1668.371 kb on + strand, within EX28DRAFT_1652 at 1668.378 kb on - strand, within EX28DRAFT_1652 at 1668.379 kb on + strand, within EX28DRAFT_1652 at 1668.425 kb on + strand, within EX28DRAFT_1652 at 1668.425 kb on + strand, within EX28DRAFT_1652 at 1668.530 kb on + strand, within EX28DRAFT_1652 at 1668.531 kb on - strand, within EX28DRAFT_1652 at 1668.606 kb on - strand at 1668.606 kb on - strand at 1668.606 kb on - strand at 1668.768 kb on + strand at 1668.769 kb on - strand at 1668.773 kb on + strand at 1668.774 kb on - strand at 1668.774 kb on - strand at 1668.815 kb on - strand at 1668.862 kb on + strand, within EX28DRAFT_1653 at 1668.889 kb on + strand, within EX28DRAFT_1653 at 1668.890 kb on - strand, within EX28DRAFT_1653 at 1668.907 kb on - strand, within EX28DRAFT_1653 at 1668.982 kb on - strand, within EX28DRAFT_1653 at 1669.168 kb on - strand, within EX28DRAFT_1653 at 1669.191 kb on + strand, within EX28DRAFT_1653 at 1669.216 kb on - strand, within EX28DRAFT_1653 at 1669.274 kb on - strand, within EX28DRAFT_1653 at 1669.276 kb on + strand, within EX28DRAFT_1653 at 1669.276 kb on + strand, within EX28DRAFT_1653 at 1669.276 kb on + strand, within EX28DRAFT_1653 at 1669.276 kb on + strand, within EX28DRAFT_1653 at 1669.277 kb on - strand, within EX28DRAFT_1653 at 1669.439 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,667,004 + EX28DRAFT_1650 0.76 -5.2 1,667,191 - +0.1 1,667,206 + +0.7 1,667,282 - EX28DRAFT_1651 0.22 -2.3 1,667,360 - EX28DRAFT_1651 0.45 -4.3 1,667,360 - EX28DRAFT_1651 0.45 -0.3 1,667,400 - EX28DRAFT_1651 0.56 -0.6 1,667,492 + EX28DRAFT_1651 0.82 -5.9 1,667,493 - EX28DRAFT_1651 0.83 +0.4 1,667,494 + EX28DRAFT_1651 0.83 -0.2 1,667,494 + EX28DRAFT_1651 0.83 -1.8 1,667,495 - EX28DRAFT_1651 0.83 -2.6 1,667,495 - EX28DRAFT_1651 0.83 +0.4 1,667,567 - +1.2 1,667,567 - +0.1 1,667,567 - +0.3 1,667,567 - -0.3 1,667,641 + -1.1 1,667,641 + -2.6 1,667,643 + +0.7 1,667,644 - -0.3 1,667,650 + +0.1 1,667,688 + +1.4 1,667,780 + -1.0 1,667,781 - +1.2 1,667,781 - +0.6 1,667,781 - +0.3 1,667,781 - +0.6 1,667,796 - +0.6 1,667,798 + +0.5 1,667,814 + -0.5 1,667,821 + +1.0 1,667,822 - +0.4 1,667,822 - -0.0 1,667,839 + +0.5 1,667,839 + +0.1 1,667,839 + -0.5 1,667,931 + -0.9 1,667,938 + -3.4 1,668,247 + EX28DRAFT_1652 0.46 +0.1 1,668,314 + EX28DRAFT_1652 0.55 -5.9 1,668,371 + EX28DRAFT_1652 0.62 +0.7 1,668,371 + EX28DRAFT_1652 0.62 +1.2 1,668,378 - EX28DRAFT_1652 0.63 +0.6 1,668,379 + EX28DRAFT_1652 0.63 -1.0 1,668,425 + EX28DRAFT_1652 0.69 +0.7 1,668,425 + EX28DRAFT_1652 0.69 -0.6 1,668,530 + EX28DRAFT_1652 0.83 -0.5 1,668,531 - EX28DRAFT_1652 0.83 -2.0 1,668,606 - +0.4 1,668,606 - +0.1 1,668,606 - +0.1 1,668,768 + -2.3 1,668,769 - -0.3 1,668,773 + +2.3 1,668,774 - +0.1 1,668,774 - +0.1 1,668,815 - +0.4 1,668,862 + EX28DRAFT_1653 0.13 +0.3 1,668,889 + EX28DRAFT_1653 0.18 +0.0 1,668,890 - EX28DRAFT_1653 0.18 -0.6 1,668,907 - EX28DRAFT_1653 0.21 +0.3 1,668,982 - EX28DRAFT_1653 0.34 -0.7 1,669,168 - EX28DRAFT_1653 0.65 +0.2 1,669,191 + EX28DRAFT_1653 0.69 +0.3 1,669,216 - EX28DRAFT_1653 0.74 +0.2 1,669,274 - EX28DRAFT_1653 0.84 -0.4 1,669,276 + EX28DRAFT_1653 0.84 -0.7 1,669,276 + EX28DRAFT_1653 0.84 +0.0 1,669,276 + EX28DRAFT_1653 0.84 +0.1 1,669,276 + EX28DRAFT_1653 0.84 -0.8 1,669,277 - EX28DRAFT_1653 0.84 +1.1 1,669,439 + -1.8
Or see this region's nucleotide sequence