Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1608 and EX28DRAFT_1609 are separated by 139 nucleotides EX28DRAFT_1609 and EX28DRAFT_1610 are separated by 199 nucleotides
EX28DRAFT_1608: EX28DRAFT_1608 - fumarase, class II (EC 4.2.1.2), at 1,624,044 to 1,625,438
_1608
EX28DRAFT_1609: EX28DRAFT_1609 - hydro-lyases, Fe-S type, tartrate/fumarate subfamily, alpha region/hydro-lyases, Fe-S type, tartrate/fumarate subfamily, beta region, at 1,625,578 to 1,627,224
_1609
EX28DRAFT_1610: EX28DRAFT_1610 - mannose-6-phosphate isomerase, type 1 (EC 5.3.1.8), at 1,627,424 to 1,628,599
_1610
Position (kb)
1625
1626
1627
1628 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1624.789 kb on + strand, within EX28DRAFT_1608 at 1624.796 kb on + strand, within EX28DRAFT_1608 at 1624.796 kb on + strand, within EX28DRAFT_1608 at 1624.797 kb on - strand, within EX28DRAFT_1608 at 1624.808 kb on + strand, within EX28DRAFT_1608 at 1624.809 kb on - strand, within EX28DRAFT_1608 at 1625.376 kb on + strand at 1625.376 kb on + strand at 1625.376 kb on + strand at 1625.488 kb on - strand at 1625.491 kb on + strand at 1625.491 kb on + strand at 1625.491 kb on + strand at 1625.491 kb on + strand at 1625.492 kb on - strand at 1625.492 kb on - strand at 1625.492 kb on - strand at 1625.579 kb on + strand at 1625.579 kb on + strand at 1625.580 kb on - strand at 1625.734 kb on - strand at 1625.967 kb on - strand, within EX28DRAFT_1609 at 1626.191 kb on + strand, within EX28DRAFT_1609 at 1626.348 kb on - strand, within EX28DRAFT_1609 at 1626.415 kb on + strand, within EX28DRAFT_1609 at 1626.415 kb on + strand, within EX28DRAFT_1609 at 1626.470 kb on + strand, within EX28DRAFT_1609 at 1626.471 kb on - strand, within EX28DRAFT_1609 at 1626.471 kb on - strand, within EX28DRAFT_1609 at 1626.471 kb on - strand, within EX28DRAFT_1609 at 1626.645 kb on - strand, within EX28DRAFT_1609 at 1626.645 kb on - strand, within EX28DRAFT_1609 at 1626.645 kb on - strand, within EX28DRAFT_1609 at 1626.645 kb on - strand, within EX28DRAFT_1609 at 1626.761 kb on + strand, within EX28DRAFT_1609 at 1626.761 kb on + strand, within EX28DRAFT_1609 at 1626.761 kb on + strand, within EX28DRAFT_1609 at 1626.762 kb on - strand, within EX28DRAFT_1609 at 1626.762 kb on - strand, within EX28DRAFT_1609 at 1626.762 kb on - strand, within EX28DRAFT_1609 at 1626.762 kb on - strand, within EX28DRAFT_1609 at 1626.766 kb on - strand, within EX28DRAFT_1609 at 1626.809 kb on + strand, within EX28DRAFT_1609 at 1626.809 kb on + strand, within EX28DRAFT_1609 at 1626.810 kb on - strand, within EX28DRAFT_1609 at 1626.819 kb on - strand, within EX28DRAFT_1609 at 1626.819 kb on - strand, within EX28DRAFT_1609 at 1626.916 kb on - strand, within EX28DRAFT_1609 at 1626.949 kb on - strand, within EX28DRAFT_1609 at 1626.972 kb on - strand, within EX28DRAFT_1609 at 1626.972 kb on - strand, within EX28DRAFT_1609 at 1626.972 kb on - strand, within EX28DRAFT_1609 at 1626.975 kb on + strand, within EX28DRAFT_1609 at 1627.044 kb on + strand, within EX28DRAFT_1609 at 1627.160 kb on + strand at 1627.160 kb on + strand at 1627.237 kb on + strand at 1627.333 kb on + strand at 1627.346 kb on + strand at 1627.359 kb on + strand at 1627.360 kb on - strand at 1627.375 kb on - strand at 1627.395 kb on + strand at 1627.404 kb on - strand at 1627.454 kb on + strand at 1627.491 kb on - strand at 1627.588 kb on - strand, within EX28DRAFT_1610 at 1627.795 kb on + strand, within EX28DRAFT_1610 at 1627.795 kb on + strand, within EX28DRAFT_1610 at 1627.799 kb on + strand, within EX28DRAFT_1610 at 1627.800 kb on - strand, within EX28DRAFT_1610 at 1627.990 kb on + strand, within EX28DRAFT_1610
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,624,789 + EX28DRAFT_1608 0.53 +0.0 1,624,796 + EX28DRAFT_1608 0.54 +0.9 1,624,796 + EX28DRAFT_1608 0.54 +0.2 1,624,797 - EX28DRAFT_1608 0.54 -0.5 1,624,808 + EX28DRAFT_1608 0.55 +0.2 1,624,809 - EX28DRAFT_1608 0.55 +0.8 1,625,376 + -0.7 1,625,376 + +0.0 1,625,376 + +2.3 1,625,488 - +0.0 1,625,491 + +0.2 1,625,491 + +0.8 1,625,491 + -1.4 1,625,491 + +0.2 1,625,492 - -2.3 1,625,492 - +0.8 1,625,492 - -0.9 1,625,579 + +0.2 1,625,579 + -0.1 1,625,580 - +0.5 1,625,734 - +0.1 1,625,967 - EX28DRAFT_1609 0.24 -0.3 1,626,191 + EX28DRAFT_1609 0.37 -0.0 1,626,348 - EX28DRAFT_1609 0.47 +0.3 1,626,415 + EX28DRAFT_1609 0.51 -1.1 1,626,415 + EX28DRAFT_1609 0.51 +1.0 1,626,470 + EX28DRAFT_1609 0.54 +0.1 1,626,471 - EX28DRAFT_1609 0.54 +0.1 1,626,471 - EX28DRAFT_1609 0.54 -0.9 1,626,471 - EX28DRAFT_1609 0.54 -1.2 1,626,645 - EX28DRAFT_1609 0.65 -0.2 1,626,645 - EX28DRAFT_1609 0.65 -0.6 1,626,645 - EX28DRAFT_1609 0.65 -0.6 1,626,645 - EX28DRAFT_1609 0.65 +2.5 1,626,761 + EX28DRAFT_1609 0.72 -0.1 1,626,761 + EX28DRAFT_1609 0.72 -3.6 1,626,761 + EX28DRAFT_1609 0.72 -1.2 1,626,762 - EX28DRAFT_1609 0.72 +0.3 1,626,762 - EX28DRAFT_1609 0.72 -0.0 1,626,762 - EX28DRAFT_1609 0.72 +0.3 1,626,762 - EX28DRAFT_1609 0.72 -0.7 1,626,766 - EX28DRAFT_1609 0.72 +0.1 1,626,809 + EX28DRAFT_1609 0.75 +0.8 1,626,809 + EX28DRAFT_1609 0.75 -2.6 1,626,810 - EX28DRAFT_1609 0.75 +0.5 1,626,819 - EX28DRAFT_1609 0.75 +0.4 1,626,819 - EX28DRAFT_1609 0.75 +0.7 1,626,916 - EX28DRAFT_1609 0.81 -0.9 1,626,949 - EX28DRAFT_1609 0.83 -1.0 1,626,972 - EX28DRAFT_1609 0.85 +0.4 1,626,972 - EX28DRAFT_1609 0.85 +0.2 1,626,972 - EX28DRAFT_1609 0.85 +0.1 1,626,975 + EX28DRAFT_1609 0.85 +0.3 1,627,044 + EX28DRAFT_1609 0.89 -0.4 1,627,160 + +2.1 1,627,160 + +0.1 1,627,237 + -1.6 1,627,333 + +0.6 1,627,346 + +0.2 1,627,359 + -0.7 1,627,360 - +0.3 1,627,375 - +1.2 1,627,395 + -0.8 1,627,404 - +0.6 1,627,454 + -0.8 1,627,491 - +1.4 1,627,588 - EX28DRAFT_1610 0.14 +1.7 1,627,795 + EX28DRAFT_1610 0.32 +1.3 1,627,795 + EX28DRAFT_1610 0.32 +0.6 1,627,799 + EX28DRAFT_1610 0.32 -0.3 1,627,800 - EX28DRAFT_1610 0.32 +1.6 1,627,990 + EX28DRAFT_1610 0.48 +0.8
Or see this region's nucleotide sequence