Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1520 and EX28DRAFT_1521 are separated by 22 nucleotides EX28DRAFT_1521 and EX28DRAFT_1522 are separated by 96 nucleotides EX28DRAFT_1522 and EX28DRAFT_1523 overlap by 29 nucleotides EX28DRAFT_1523 and EX28DRAFT_1524 are separated by 48 nucleotides
EX28DRAFT_1520: EX28DRAFT_1520 - Cation/multidrug efflux pump, at 1,533,583 to 1,536,660
_1520
EX28DRAFT_1521: EX28DRAFT_1521 - Arylamine N-acetyltransferase, at 1,536,683 to 1,537,450
_1521
EX28DRAFT_1522: EX28DRAFT_1522 - Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs, at 1,537,547 to 1,539,001
_1522
EX28DRAFT_1523: EX28DRAFT_1523 - Transcriptional regulator, at 1,538,973 to 1,539,944
_1523
EX28DRAFT_1524: EX28DRAFT_1524 - Dioxygenases related to 2-nitropropane dioxygenase, at 1,539,993 to 1,540,973
_1524
Position (kb)
1537
1538
1539
1540 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1536.614 kb on + strand at 1536.871 kb on + strand, within EX28DRAFT_1521 at 1536.872 kb on - strand, within EX28DRAFT_1521 at 1536.876 kb on + strand, within EX28DRAFT_1521 at 1536.876 kb on + strand, within EX28DRAFT_1521 at 1536.877 kb on - strand, within EX28DRAFT_1521 at 1536.877 kb on - strand, within EX28DRAFT_1521 at 1536.877 kb on - strand, within EX28DRAFT_1521 at 1536.877 kb on - strand, within EX28DRAFT_1521 at 1536.969 kb on + strand, within EX28DRAFT_1521 at 1537.233 kb on + strand, within EX28DRAFT_1521 at 1537.234 kb on - strand, within EX28DRAFT_1521 at 1537.234 kb on - strand, within EX28DRAFT_1521 at 1537.234 kb on - strand, within EX28DRAFT_1521 at 1537.234 kb on - strand, within EX28DRAFT_1521 at 1537.234 kb on - strand, within EX28DRAFT_1521 at 1537.402 kb on - strand at 1537.484 kb on + strand at 1537.484 kb on + strand at 1537.484 kb on + strand at 1537.484 kb on + strand at 1537.485 kb on - strand at 1537.485 kb on - strand at 1537.485 kb on - strand at 1537.485 kb on - strand at 1537.485 kb on - strand at 1537.485 kb on - strand at 1537.485 kb on - strand at 1537.507 kb on + strand at 1537.507 kb on + strand at 1537.508 kb on - strand at 1537.585 kb on + strand at 1537.607 kb on - strand at 1537.613 kb on - strand at 1537.761 kb on - strand, within EX28DRAFT_1522 at 1538.563 kb on - strand, within EX28DRAFT_1522 at 1538.643 kb on - strand, within EX28DRAFT_1522 at 1538.874 kb on + strand at 1539.105 kb on + strand, within EX28DRAFT_1523 at 1539.105 kb on + strand, within EX28DRAFT_1523 at 1539.105 kb on + strand, within EX28DRAFT_1523 at 1539.106 kb on - strand, within EX28DRAFT_1523 at 1539.106 kb on - strand, within EX28DRAFT_1523 at 1539.106 kb on - strand, within EX28DRAFT_1523 at 1539.109 kb on + strand, within EX28DRAFT_1523 at 1539.109 kb on + strand, within EX28DRAFT_1523 at 1539.110 kb on - strand, within EX28DRAFT_1523 at 1539.110 kb on - strand, within EX28DRAFT_1523 at 1539.110 kb on - strand, within EX28DRAFT_1523 at 1539.173 kb on - strand, within EX28DRAFT_1523 at 1539.173 kb on - strand, within EX28DRAFT_1523 at 1539.213 kb on - strand, within EX28DRAFT_1523 at 1539.239 kb on + strand, within EX28DRAFT_1523 at 1539.239 kb on + strand, within EX28DRAFT_1523 at 1539.240 kb on - strand, within EX28DRAFT_1523 at 1539.272 kb on + strand, within EX28DRAFT_1523 at 1539.395 kb on + strand, within EX28DRAFT_1523 at 1539.397 kb on + strand, within EX28DRAFT_1523 at 1539.398 kb on - strand, within EX28DRAFT_1523 at 1539.585 kb on + strand, within EX28DRAFT_1523 at 1539.585 kb on + strand, within EX28DRAFT_1523 at 1539.616 kb on - strand, within EX28DRAFT_1523 at 1539.684 kb on - strand, within EX28DRAFT_1523 at 1539.955 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,536,614 + +0.1 1,536,871 + EX28DRAFT_1521 0.24 -0.2 1,536,872 - EX28DRAFT_1521 0.25 +0.0 1,536,876 + EX28DRAFT_1521 0.25 +1.0 1,536,876 + EX28DRAFT_1521 0.25 -0.5 1,536,877 - EX28DRAFT_1521 0.25 +0.3 1,536,877 - EX28DRAFT_1521 0.25 -0.5 1,536,877 - EX28DRAFT_1521 0.25 -0.1 1,536,877 - EX28DRAFT_1521 0.25 -1.1 1,536,969 + EX28DRAFT_1521 0.37 +0.5 1,537,233 + EX28DRAFT_1521 0.72 +0.8 1,537,234 - EX28DRAFT_1521 0.72 +0.9 1,537,234 - EX28DRAFT_1521 0.72 +0.9 1,537,234 - EX28DRAFT_1521 0.72 -0.4 1,537,234 - EX28DRAFT_1521 0.72 -1.1 1,537,234 - EX28DRAFT_1521 0.72 +2.0 1,537,402 - -0.0 1,537,484 + -0.1 1,537,484 + -0.1 1,537,484 + -0.3 1,537,484 + -2.9 1,537,485 - +1.4 1,537,485 - +0.8 1,537,485 - +0.3 1,537,485 - -0.7 1,537,485 - -0.7 1,537,485 - +0.3 1,537,485 - +1.0 1,537,507 + -2.4 1,537,507 + +0.8 1,537,508 - +0.5 1,537,585 + -1.9 1,537,607 - +0.1 1,537,613 - +0.2 1,537,761 - EX28DRAFT_1522 0.15 -1.4 1,538,563 - EX28DRAFT_1522 0.70 -0.2 1,538,643 - EX28DRAFT_1522 0.75 -0.4 1,538,874 + -0.8 1,539,105 + EX28DRAFT_1523 0.14 -0.1 1,539,105 + EX28DRAFT_1523 0.14 -0.5 1,539,105 + EX28DRAFT_1523 0.14 +0.1 1,539,106 - EX28DRAFT_1523 0.14 -1.4 1,539,106 - EX28DRAFT_1523 0.14 +0.7 1,539,106 - EX28DRAFT_1523 0.14 -0.1 1,539,109 + EX28DRAFT_1523 0.14 -0.2 1,539,109 + EX28DRAFT_1523 0.14 +0.2 1,539,110 - EX28DRAFT_1523 0.14 -1.1 1,539,110 - EX28DRAFT_1523 0.14 -0.9 1,539,110 - EX28DRAFT_1523 0.14 +0.5 1,539,173 - EX28DRAFT_1523 0.21 -0.5 1,539,173 - EX28DRAFT_1523 0.21 +0.9 1,539,213 - EX28DRAFT_1523 0.25 +1.0 1,539,239 + EX28DRAFT_1523 0.27 +0.6 1,539,239 + EX28DRAFT_1523 0.27 +0.1 1,539,240 - EX28DRAFT_1523 0.27 +2.1 1,539,272 + EX28DRAFT_1523 0.31 +0.6 1,539,395 + EX28DRAFT_1523 0.43 -0.4 1,539,397 + EX28DRAFT_1523 0.44 -4.1 1,539,398 - EX28DRAFT_1523 0.44 +0.4 1,539,585 + EX28DRAFT_1523 0.63 +1.1 1,539,585 + EX28DRAFT_1523 0.63 -1.4 1,539,616 - EX28DRAFT_1523 0.66 -0.8 1,539,684 - EX28DRAFT_1523 0.73 -0.5 1,539,955 + +1.1
Or see this region's nucleotide sequence