Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1417 and EX28DRAFT_1418 are separated by 11 nucleotides EX28DRAFT_1418 and EX28DRAFT_1419 are separated by 8 nucleotides EX28DRAFT_1419 and EX28DRAFT_1420 are separated by 11 nucleotides
EX28DRAFT_1417: EX28DRAFT_1417 - phenylacetate-CoA oxygenase, PaaJ subunit, at 1,425,373 to 1,425,870
_1417
EX28DRAFT_1418: EX28DRAFT_1418 - phenylacetate-CoA oxygenase, PaaI subunit, at 1,425,882 to 1,426,628
_1418
EX28DRAFT_1419: EX28DRAFT_1419 - phenylacetate-CoA oxygenase, PaaH subunit, at 1,426,637 to 1,426,924
_1419
EX28DRAFT_1420: EX28DRAFT_1420 - phenylacetate-CoA oxygenase, PaaG subunit, at 1,426,936 to 1,427,865
_1420
Position (kb) 1426
1427 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1425.704 kb on + strand, within EX28DRAFT_1417 at 1425.705 kb on - strand, within EX28DRAFT_1417 at 1425.705 kb on - strand, within EX28DRAFT_1417 at 1425.983 kb on - strand, within EX28DRAFT_1418 at 1425.987 kb on + strand, within EX28DRAFT_1418 at 1426.248 kb on - strand, within EX28DRAFT_1418 at 1426.465 kb on + strand, within EX28DRAFT_1418 at 1426.466 kb on - strand, within EX28DRAFT_1418 at 1426.642 kb on + strand at 1426.642 kb on + strand at 1426.642 kb on + strand at 1426.643 kb on - strand at 1426.643 kb on - strand at 1426.643 kb on - strand at 1426.643 kb on - strand at 1426.771 kb on + strand, within EX28DRAFT_1419 at 1426.771 kb on + strand, within EX28DRAFT_1419 at 1426.772 kb on - strand, within EX28DRAFT_1419 at 1426.886 kb on + strand, within EX28DRAFT_1419 at 1427.572 kb on + strand, within EX28DRAFT_1420 at 1427.586 kb on - strand, within EX28DRAFT_1420
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,425,704 + EX28DRAFT_1417 0.66 +0.3 1,425,705 - EX28DRAFT_1417 0.67 -2.0 1,425,705 - EX28DRAFT_1417 0.67 +0.4 1,425,983 - EX28DRAFT_1418 0.14 -1.4 1,425,987 + EX28DRAFT_1418 0.14 +0.8 1,426,248 - EX28DRAFT_1418 0.49 +1.2 1,426,465 + EX28DRAFT_1418 0.78 +0.1 1,426,466 - EX28DRAFT_1418 0.78 +0.3 1,426,642 + -3.2 1,426,642 + +0.3 1,426,642 + -0.6 1,426,643 - -0.4 1,426,643 - -1.4 1,426,643 - -0.6 1,426,643 - -2.4 1,426,771 + EX28DRAFT_1419 0.47 +0.3 1,426,771 + EX28DRAFT_1419 0.47 -0.9 1,426,772 - EX28DRAFT_1419 0.47 -0.5 1,426,886 + EX28DRAFT_1419 0.86 +0.2 1,427,572 + EX28DRAFT_1420 0.68 -2.2 1,427,586 - EX28DRAFT_1420 0.70 +0.6
Or see this region's nucleotide sequence