Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0997 and EX28DRAFT_0998 overlap by 10 nucleotides EX28DRAFT_0998 and EX28DRAFT_0999 are separated by 185 nucleotides
EX28DRAFT_0997: EX28DRAFT_0997 - L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily, at 1,000,481 to 1,001,446
_0997
EX28DRAFT_0998: EX28DRAFT_0998 - Predicted carboxypeptidase, at 1,001,437 to 1,002,150
_0998
EX28DRAFT_0999: EX28DRAFT_0999 - ABC-type oligopeptide transport system, periplasmic component, at 1,002,336 to 1,003,952
_0999
Position (kb)
1001
1002
1003 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1000.500 kb on - strand at 1000.523 kb on - strand at 1000.615 kb on + strand, within EX28DRAFT_0997 at 1000.616 kb on - strand, within EX28DRAFT_0997 at 1000.664 kb on - strand, within EX28DRAFT_0997 at 1001.479 kb on + strand at 1001.712 kb on + strand, within EX28DRAFT_0998 at 1001.713 kb on - strand, within EX28DRAFT_0998 at 1001.713 kb on - strand, within EX28DRAFT_0998 at 1001.854 kb on - strand, within EX28DRAFT_0998 at 1002.142 kb on - strand at 1002.292 kb on - strand at 1002.292 kb on - strand at 1002.335 kb on + strand at 1002.335 kb on + strand at 1002.336 kb on - strand at 1002.336 kb on - strand at 1002.372 kb on + strand at 1002.390 kb on - strand at 1002.458 kb on + strand at 1002.458 kb on + strand at 1002.462 kb on - strand at 1002.462 kb on - strand at 1002.572 kb on + strand, within EX28DRAFT_0999 at 1002.801 kb on - strand, within EX28DRAFT_0999 at 1002.897 kb on + strand, within EX28DRAFT_0999 at 1003.100 kb on + strand, within EX28DRAFT_0999 at 1003.122 kb on - strand, within EX28DRAFT_0999
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,000,500 - -0.6 1,000,523 - -0.6 1,000,615 + EX28DRAFT_0997 0.14 +0.9 1,000,616 - EX28DRAFT_0997 0.14 +1.0 1,000,664 - EX28DRAFT_0997 0.19 -0.1 1,001,479 + -0.2 1,001,712 + EX28DRAFT_0998 0.39 -3.8 1,001,713 - EX28DRAFT_0998 0.39 -0.9 1,001,713 - EX28DRAFT_0998 0.39 -0.2 1,001,854 - EX28DRAFT_0998 0.58 -1.2 1,002,142 - -0.8 1,002,292 - -2.9 1,002,292 - -0.3 1,002,335 + +0.5 1,002,335 + -1.9 1,002,336 - -1.9 1,002,336 - +0.1 1,002,372 + +0.1 1,002,390 - -0.1 1,002,458 + +0.7 1,002,458 + +0.2 1,002,462 - +0.4 1,002,462 - +0.0 1,002,572 + EX28DRAFT_0999 0.15 +0.2 1,002,801 - EX28DRAFT_0999 0.29 -2.3 1,002,897 + EX28DRAFT_0999 0.35 +0.3 1,003,100 + EX28DRAFT_0999 0.47 +2.1 1,003,122 - EX28DRAFT_0999 0.49 +1.1
Or see this region's nucleotide sequence