Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0628 and EX28DRAFT_0629 are separated by 21 nucleotides EX28DRAFT_0629 and EX28DRAFT_0630 overlap by 4 nucleotides EX28DRAFT_0630 and EX28DRAFT_0631 are separated by 221 nucleotides
EX28DRAFT_0628: EX28DRAFT_0628 - D-cysteine desulfhydrase (EC 4.4.1.15), at 625,277 to 626,263
_0628
EX28DRAFT_0629: EX28DRAFT_0629 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 626,285 to 626,953
_0629
EX28DRAFT_0630: EX28DRAFT_0630 - ABC-type polar amino acid transport system, ATPase component, at 626,950 to 627,702
_0630
EX28DRAFT_0631: EX28DRAFT_0631 - DNA-binding HTH domain-containing proteins, at 627,924 to 628,646
_0631
Position (kb) 626
627 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 625.343 kb on + strand at 625.364 kb on + strand at 625.368 kb on + strand at 625.378 kb on + strand, within EX28DRAFT_0628 at 625.427 kb on - strand, within EX28DRAFT_0628 at 625.642 kb on + strand, within EX28DRAFT_0628 at 625.643 kb on - strand, within EX28DRAFT_0628 at 625.643 kb on - strand, within EX28DRAFT_0628 at 625.736 kb on + strand, within EX28DRAFT_0628 at 625.781 kb on + strand, within EX28DRAFT_0628 at 625.977 kb on + strand, within EX28DRAFT_0628 at 625.977 kb on + strand, within EX28DRAFT_0628 at 626.042 kb on + strand, within EX28DRAFT_0628 at 626.042 kb on + strand, within EX28DRAFT_0628 at 626.042 kb on + strand, within EX28DRAFT_0628 at 626.043 kb on - strand, within EX28DRAFT_0628 at 626.343 kb on + strand at 626.348 kb on - strand at 626.348 kb on - strand at 626.410 kb on - strand, within EX28DRAFT_0629 at 626.457 kb on - strand, within EX28DRAFT_0629 at 626.457 kb on - strand, within EX28DRAFT_0629 at 626.457 kb on - strand, within EX28DRAFT_0629 at 626.458 kb on + strand, within EX28DRAFT_0629 at 626.458 kb on + strand, within EX28DRAFT_0629 at 626.459 kb on - strand, within EX28DRAFT_0629 at 626.500 kb on + strand, within EX28DRAFT_0629 at 626.500 kb on + strand, within EX28DRAFT_0629 at 626.507 kb on + strand, within EX28DRAFT_0629 at 626.507 kb on + strand, within EX28DRAFT_0629 at 626.507 kb on + strand, within EX28DRAFT_0629 at 626.508 kb on - strand, within EX28DRAFT_0629 at 626.588 kb on + strand, within EX28DRAFT_0629 at 626.588 kb on + strand, within EX28DRAFT_0629 at 626.761 kb on + strand, within EX28DRAFT_0629 at 626.762 kb on - strand, within EX28DRAFT_0629 at 626.762 kb on - strand, within EX28DRAFT_0629 at 626.850 kb on - strand, within EX28DRAFT_0629 at 626.850 kb on - strand, within EX28DRAFT_0629 at 626.850 kb on - strand, within EX28DRAFT_0629 at 626.850 kb on - strand, within EX28DRAFT_0629 at 626.850 kb on - strand, within EX28DRAFT_0629 at 626.850 kb on - strand, within EX28DRAFT_0629 at 627.094 kb on - strand, within EX28DRAFT_0630 at 627.094 kb on - strand, within EX28DRAFT_0630 at 627.094 kb on - strand, within EX28DRAFT_0630 at 627.094 kb on - strand, within EX28DRAFT_0630 at 627.097 kb on - strand, within EX28DRAFT_0630 at 627.186 kb on - strand, within EX28DRAFT_0630 at 627.268 kb on - strand, within EX28DRAFT_0630 at 627.287 kb on + strand, within EX28DRAFT_0630 at 627.288 kb on - strand, within EX28DRAFT_0630 at 627.339 kb on + strand, within EX28DRAFT_0630 at 627.371 kb on - strand, within EX28DRAFT_0630 at 627.732 kb on + strand at 627.806 kb on - strand at 627.815 kb on - strand at 627.869 kb on + strand at 627.870 kb on - strand at 627.870 kb on - strand at 627.870 kb on - strand at 627.870 kb on - strand at 627.875 kb on - strand at 627.876 kb on + strand at 627.885 kb on + strand at 627.886 kb on - strand at 627.888 kb on + strand at 627.889 kb on - strand at 627.896 kb on - strand at 627.896 kb on - strand at 627.896 kb on - strand at 627.917 kb on - strand at 627.919 kb on + strand at 627.920 kb on - strand at 627.920 kb on - strand at 627.920 kb on - strand at 627.920 kb on - strand at 627.923 kb on + strand at 627.924 kb on - strand at 627.924 kb on - strand at 627.924 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 625,343 + +1.8 625,364 + -0.1 625,368 + +0.3 625,378 + EX28DRAFT_0628 0.10 +0.2 625,427 - EX28DRAFT_0628 0.15 -1.6 625,642 + EX28DRAFT_0628 0.37 -4.1 625,643 - EX28DRAFT_0628 0.37 +0.2 625,643 - EX28DRAFT_0628 0.37 -2.1 625,736 + EX28DRAFT_0628 0.47 -0.4 625,781 + EX28DRAFT_0628 0.51 +0.3 625,977 + EX28DRAFT_0628 0.71 +0.4 625,977 + EX28DRAFT_0628 0.71 +0.8 626,042 + EX28DRAFT_0628 0.78 -0.9 626,042 + EX28DRAFT_0628 0.78 +1.9 626,042 + EX28DRAFT_0628 0.78 +0.8 626,043 - EX28DRAFT_0628 0.78 -0.4 626,343 + -1.0 626,348 - -0.2 626,348 - +0.1 626,410 - EX28DRAFT_0629 0.19 +0.7 626,457 - EX28DRAFT_0629 0.26 +0.0 626,457 - EX28DRAFT_0629 0.26 +1.2 626,457 - EX28DRAFT_0629 0.26 -4.0 626,458 + EX28DRAFT_0629 0.26 +2.8 626,458 + EX28DRAFT_0629 0.26 -1.1 626,459 - EX28DRAFT_0629 0.26 -0.6 626,500 + EX28DRAFT_0629 0.32 -0.1 626,500 + EX28DRAFT_0629 0.32 -1.7 626,507 + EX28DRAFT_0629 0.33 -1.7 626,507 + EX28DRAFT_0629 0.33 -3.6 626,507 + EX28DRAFT_0629 0.33 -2.1 626,508 - EX28DRAFT_0629 0.33 +0.5 626,588 + EX28DRAFT_0629 0.45 +1.2 626,588 + EX28DRAFT_0629 0.45 -0.4 626,761 + EX28DRAFT_0629 0.71 -0.1 626,762 - EX28DRAFT_0629 0.71 +0.1 626,762 - EX28DRAFT_0629 0.71 -0.6 626,850 - EX28DRAFT_0629 0.84 +0.2 626,850 - EX28DRAFT_0629 0.84 -0.3 626,850 - EX28DRAFT_0629 0.84 -0.2 626,850 - EX28DRAFT_0629 0.84 -2.1 626,850 - EX28DRAFT_0629 0.84 -1.1 626,850 - EX28DRAFT_0629 0.84 -2.4 627,094 - EX28DRAFT_0630 0.19 +1.2 627,094 - EX28DRAFT_0630 0.19 -2.7 627,094 - EX28DRAFT_0630 0.19 +0.4 627,094 - EX28DRAFT_0630 0.19 -1.1 627,097 - EX28DRAFT_0630 0.20 -0.6 627,186 - EX28DRAFT_0630 0.31 -1.1 627,268 - EX28DRAFT_0630 0.42 -0.4 627,287 + EX28DRAFT_0630 0.45 +1.2 627,288 - EX28DRAFT_0630 0.45 -0.4 627,339 + EX28DRAFT_0630 0.52 +1.3 627,371 - EX28DRAFT_0630 0.56 -3.9 627,732 + -0.2 627,806 - +0.9 627,815 - +0.6 627,869 + -0.2 627,870 - +0.9 627,870 - -0.2 627,870 - +0.8 627,870 - +1.0 627,875 - +1.8 627,876 + -4.6 627,885 + +1.2 627,886 - +0.2 627,888 + -0.1 627,889 - +0.9 627,896 - -0.1 627,896 - -0.1 627,896 - -0.3 627,917 - +0.8 627,919 + +0.7 627,920 - -0.1 627,920 - -0.1 627,920 - +1.8 627,920 - -0.0 627,923 + +0.8 627,924 - +0.3 627,924 - -0.1 627,924 - -0.8
Or see this region's nucleotide sequence