Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0614 and EX28DRAFT_0615 overlap by 8 nucleotides EX28DRAFT_0615 and EX28DRAFT_0616 overlap by 8 nucleotides EX28DRAFT_0616 and EX28DRAFT_0617 are separated by 228 nucleotides EX28DRAFT_0617 and EX28DRAFT_0618 are separated by 74 nucleotides
EX28DRAFT_0614: EX28DRAFT_0614 - Flagellar biosynthesis/type III secretory pathway protein, at 612,065 to 612,772
_0614
EX28DRAFT_0615: EX28DRAFT_0615 - flagellar motor switch protein FliG, at 612,765 to 613,763
_0615
EX28DRAFT_0616: EX28DRAFT_0616 - flagellar basal-body M-ring protein/flagellar hook-basal body protein (fliF), at 613,756 to 615,432
_0616
EX28DRAFT_0617: EX28DRAFT_0617 - flagellar hook-basal body complex protein FliE, at 615,661 to 615,975
_0617
EX28DRAFT_0618: EX28DRAFT_0618 - YedD-like protein, at 616,050 to 616,463
_0618
Position (kb) 613
614
615
616 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 612.766 kb on + strand at 612.767 kb on - strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 613.424 kb on + strand, within EX28DRAFT_0615 at 613.534 kb on + strand, within EX28DRAFT_0615 at 613.754 kb on + strand at 613.754 kb on + strand at 613.755 kb on - strand at 613.755 kb on - strand at 613.755 kb on - strand at 613.757 kb on + strand at 613.757 kb on + strand at 613.757 kb on + strand at 613.758 kb on - strand at 613.758 kb on - strand at 613.815 kb on + strand at 614.261 kb on + strand, within EX28DRAFT_0616 at 614.261 kb on + strand, within EX28DRAFT_0616 at 614.261 kb on + strand, within EX28DRAFT_0616 at 614.261 kb on + strand, within EX28DRAFT_0616 at 614.262 kb on - strand, within EX28DRAFT_0616 at 614.313 kb on + strand, within EX28DRAFT_0616 at 614.313 kb on + strand, within EX28DRAFT_0616 at 614.313 kb on + strand, within EX28DRAFT_0616 at 614.314 kb on - strand, within EX28DRAFT_0616 at 614.342 kb on + strand, within EX28DRAFT_0616 at 614.342 kb on + strand, within EX28DRAFT_0616 at 614.343 kb on - strand, within EX28DRAFT_0616 at 614.343 kb on - strand, within EX28DRAFT_0616 at 614.358 kb on + strand, within EX28DRAFT_0616 at 614.481 kb on + strand, within EX28DRAFT_0616 at 614.589 kb on - strand, within EX28DRAFT_0616 at 614.610 kb on + strand, within EX28DRAFT_0616 at 614.723 kb on + strand, within EX28DRAFT_0616 at 614.724 kb on - strand, within EX28DRAFT_0616 at 614.724 kb on - strand, within EX28DRAFT_0616 at 614.941 kb on + strand, within EX28DRAFT_0616 at 614.993 kb on - strand, within EX28DRAFT_0616 at 615.039 kb on - strand, within EX28DRAFT_0616 at 615.039 kb on - strand, within EX28DRAFT_0616 at 615.159 kb on + strand, within EX28DRAFT_0616 at 615.159 kb on + strand, within EX28DRAFT_0616 at 615.159 kb on + strand, within EX28DRAFT_0616 at 615.160 kb on - strand, within EX28DRAFT_0616 at 615.160 kb on - strand, within EX28DRAFT_0616 at 615.160 kb on - strand, within EX28DRAFT_0616 at 615.160 kb on - strand, within EX28DRAFT_0616 at 615.313 kb on + strand at 615.313 kb on + strand at 615.399 kb on + strand at 615.445 kb on - strand at 615.445 kb on - strand at 615.459 kb on + strand at 615.459 kb on + strand at 615.459 kb on + strand at 615.460 kb on - strand at 615.460 kb on - strand at 615.460 kb on - strand at 615.561 kb on + strand at 615.572 kb on + strand at 615.591 kb on + strand at 615.592 kb on - strand at 615.592 kb on - strand at 615.595 kb on + strand at 615.604 kb on + strand at 615.610 kb on - strand at 615.623 kb on + strand at 615.624 kb on - strand at 615.638 kb on - strand at 615.666 kb on + strand at 615.667 kb on - strand at 615.669 kb on - strand at 615.946 kb on + strand at 616.113 kb on + strand, within EX28DRAFT_0618 at 616.113 kb on + strand, within EX28DRAFT_0618 at 616.113 kb on + strand, within EX28DRAFT_0618 at 616.113 kb on + strand, within EX28DRAFT_0618 at 616.114 kb on - strand, within EX28DRAFT_0618 at 616.309 kb on - strand, within EX28DRAFT_0618 at 616.309 kb on - strand, within EX28DRAFT_0618 at 616.338 kb on + strand, within EX28DRAFT_0618
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 612,766 + +0.5 612,767 - +0.5 612,772 + -0.2 612,772 + +1.0 612,772 + -0.5 612,772 + -0.6 612,772 + -0.1 612,772 + +0.3 612,772 + +1.1 612,772 + +1.0 612,772 + -0.8 612,772 + -0.1 612,772 + -0.1 612,772 + +0.3 612,772 + +0.5 612,772 + +1.2 612,772 + -0.5 612,772 + -0.1 612,772 + +2.7 612,772 + -0.1 612,772 + +0.9 612,773 - -0.0 612,773 - +1.7 612,773 - +0.7 612,773 - -1.0 612,773 - -0.8 612,773 - -1.1 612,773 - +0.3 612,773 - +2.8 612,773 - -1.8 612,773 - -0.0 612,773 - -0.1 612,773 - +0.1 612,773 - +0.7 612,773 - +0.4 612,773 - +0.5 612,773 - +0.2 612,773 - -2.7 612,773 - +1.7 613,424 + EX28DRAFT_0615 0.66 -0.8 613,534 + EX28DRAFT_0615 0.77 +0.2 613,754 + -0.7 613,754 + -0.3 613,755 - +0.2 613,755 - +0.3 613,755 - -0.3 613,757 + +0.4 613,757 + +0.8 613,757 + +0.0 613,758 - +0.6 613,758 - -0.5 613,815 + -0.9 614,261 + EX28DRAFT_0616 0.30 -0.1 614,261 + EX28DRAFT_0616 0.30 +0.1 614,261 + EX28DRAFT_0616 0.30 +1.5 614,261 + EX28DRAFT_0616 0.30 +0.2 614,262 - EX28DRAFT_0616 0.30 +0.6 614,313 + EX28DRAFT_0616 0.33 -1.1 614,313 + EX28DRAFT_0616 0.33 -0.5 614,313 + EX28DRAFT_0616 0.33 +0.3 614,314 - EX28DRAFT_0616 0.33 +0.3 614,342 + EX28DRAFT_0616 0.35 +0.4 614,342 + EX28DRAFT_0616 0.35 +1.1 614,343 - EX28DRAFT_0616 0.35 -0.1 614,343 - EX28DRAFT_0616 0.35 +1.7 614,358 + EX28DRAFT_0616 0.36 -0.0 614,481 + EX28DRAFT_0616 0.43 +0.1 614,589 - EX28DRAFT_0616 0.50 +0.7 614,610 + EX28DRAFT_0616 0.51 +1.1 614,723 + EX28DRAFT_0616 0.58 +0.3 614,724 - EX28DRAFT_0616 0.58 +0.3 614,724 - EX28DRAFT_0616 0.58 -0.2 614,941 + EX28DRAFT_0616 0.71 +1.7 614,993 - EX28DRAFT_0616 0.74 +0.4 615,039 - EX28DRAFT_0616 0.77 -0.1 615,039 - EX28DRAFT_0616 0.77 -0.2 615,159 + EX28DRAFT_0616 0.84 -0.1 615,159 + EX28DRAFT_0616 0.84 +0.9 615,159 + EX28DRAFT_0616 0.84 -0.1 615,160 - EX28DRAFT_0616 0.84 +0.3 615,160 - EX28DRAFT_0616 0.84 +0.1 615,160 - EX28DRAFT_0616 0.84 +0.1 615,160 - EX28DRAFT_0616 0.84 +0.4 615,313 + +1.1 615,313 + +0.0 615,399 + -0.6 615,445 - +0.8 615,445 - +0.8 615,459 + +1.5 615,459 + +0.9 615,459 + -0.1 615,460 - +0.4 615,460 - +0.4 615,460 - +1.4 615,561 + +0.7 615,572 + +0.5 615,591 + +0.3 615,592 - +0.5 615,592 - +0.2 615,595 + +0.2 615,604 + +0.3 615,610 - +0.5 615,623 + +0.3 615,624 - +0.4 615,638 - -0.3 615,666 + +1.2 615,667 - +0.3 615,669 - +0.3 615,946 + +0.7 616,113 + EX28DRAFT_0618 0.15 +2.2 616,113 + EX28DRAFT_0618 0.15 -0.0 616,113 + EX28DRAFT_0618 0.15 +1.0 616,113 + EX28DRAFT_0618 0.15 -0.7 616,114 - EX28DRAFT_0618 0.15 -1.5 616,309 - EX28DRAFT_0618 0.63 -0.3 616,309 - EX28DRAFT_0618 0.63 +0.5 616,338 + EX28DRAFT_0618 0.70 -2.9
Or see this region's nucleotide sequence