Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0423 and EX28DRAFT_0424 are separated by 84 nucleotides EX28DRAFT_0424 and EX28DRAFT_0425 are separated by 157 nucleotides EX28DRAFT_0425 and EX28DRAFT_0426 are separated by 167 nucleotides
EX28DRAFT_0423: EX28DRAFT_0423 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 403,089 to 403,853
_0423
EX28DRAFT_0424: EX28DRAFT_0424 - Uncharacterized membrane-associated protein, at 403,938 to 404,510
_0424
EX28DRAFT_0425: EX28DRAFT_0425 - Protein of unknown function (DUF1282), at 404,668 to 405,255
_0425
EX28DRAFT_0426: EX28DRAFT_0426 - D-alanyl-D-alanine carboxypeptidase, at 405,423 to 406,346
_0426
Position (kb) 404
405
406 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 403.799 kb on + strand at 403.852 kb on - strand at 403.911 kb on - strand at 404.207 kb on + strand, within EX28DRAFT_0424 at 404.207 kb on + strand, within EX28DRAFT_0424 at 404.208 kb on - strand, within EX28DRAFT_0424 at 404.208 kb on - strand, within EX28DRAFT_0424 at 404.208 kb on - strand, within EX28DRAFT_0424 at 404.234 kb on - strand, within EX28DRAFT_0424 at 404.332 kb on + strand, within EX28DRAFT_0424 at 404.492 kb on + strand at 404.493 kb on - strand at 404.511 kb on + strand at 404.511 kb on + strand at 404.511 kb on + strand at 404.523 kb on - strand at 404.523 kb on - strand at 404.546 kb on - strand at 404.551 kb on + strand at 404.551 kb on + strand at 404.551 kb on + strand at 404.551 kb on + strand at 404.552 kb on - strand at 404.552 kb on - strand at 404.553 kb on + strand at 404.553 kb on + strand at 404.553 kb on + strand at 404.554 kb on - strand at 404.554 kb on - strand at 404.625 kb on + strand at 404.625 kb on + strand at 404.627 kb on + strand at 404.627 kb on + strand at 404.627 kb on + strand at 404.628 kb on - strand at 404.628 kb on - strand at 404.628 kb on - strand at 404.631 kb on + strand at 404.632 kb on - strand at 404.632 kb on - strand at 404.792 kb on + strand, within EX28DRAFT_0425 at 404.792 kb on + strand, within EX28DRAFT_0425 at 405.023 kb on + strand, within EX28DRAFT_0425 at 405.078 kb on + strand, within EX28DRAFT_0425 at 405.079 kb on - strand, within EX28DRAFT_0425 at 405.079 kb on - strand, within EX28DRAFT_0425 at 405.091 kb on + strand, within EX28DRAFT_0425 at 405.092 kb on - strand, within EX28DRAFT_0425 at 405.092 kb on - strand, within EX28DRAFT_0425 at 405.109 kb on + strand, within EX28DRAFT_0425 at 405.142 kb on - strand, within EX28DRAFT_0425 at 405.276 kb on - strand at 405.383 kb on + strand at 405.403 kb on + strand at 405.404 kb on - strand at 405.450 kb on - strand at 405.578 kb on + strand, within EX28DRAFT_0426 at 405.588 kb on + strand, within EX28DRAFT_0426 at 405.589 kb on - strand, within EX28DRAFT_0426 at 405.637 kb on + strand, within EX28DRAFT_0426 at 405.730 kb on - strand, within EX28DRAFT_0426 at 405.906 kb on + strand, within EX28DRAFT_0426 at 405.907 kb on - strand, within EX28DRAFT_0426 at 406.052 kb on + strand, within EX28DRAFT_0426 at 406.053 kb on - strand, within EX28DRAFT_0426 at 406.053 kb on - strand, within EX28DRAFT_0426 at 406.053 kb on - strand, within EX28DRAFT_0426 at 406.053 kb on - strand, within EX28DRAFT_0426 at 406.053 kb on - strand, within EX28DRAFT_0426 at 406.053 kb on - strand, within EX28DRAFT_0426 at 406.074 kb on - strand, within EX28DRAFT_0426 at 406.084 kb on + strand, within EX28DRAFT_0426 at 406.086 kb on + strand, within EX28DRAFT_0426 at 406.176 kb on - strand, within EX28DRAFT_0426 at 406.215 kb on + strand, within EX28DRAFT_0426 at 406.215 kb on + strand, within EX28DRAFT_0426 at 406.215 kb on + strand, within EX28DRAFT_0426 at 406.215 kb on + strand, within EX28DRAFT_0426 at 406.215 kb on + strand, within EX28DRAFT_0426 at 406.216 kb on - strand, within EX28DRAFT_0426 at 406.247 kb on + strand, within EX28DRAFT_0426 at 406.248 kb on - strand, within EX28DRAFT_0426
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 403,799 + -1.0 403,852 - +0.5 403,911 - +0.4 404,207 + EX28DRAFT_0424 0.47 +2.4 404,207 + EX28DRAFT_0424 0.47 +0.1 404,208 - EX28DRAFT_0424 0.47 +0.4 404,208 - EX28DRAFT_0424 0.47 -0.0 404,208 - EX28DRAFT_0424 0.47 -0.0 404,234 - EX28DRAFT_0424 0.52 -0.2 404,332 + EX28DRAFT_0424 0.69 -0.6 404,492 + +1.3 404,493 - -0.9 404,511 + +0.9 404,511 + +0.9 404,511 + -3.7 404,523 - +1.6 404,523 - +0.3 404,546 - +0.9 404,551 + +0.0 404,551 + -0.6 404,551 + -0.1 404,551 + -0.5 404,552 - -1.1 404,552 - +0.6 404,553 + +0.5 404,553 + -0.1 404,553 + -0.9 404,554 - -0.4 404,554 - -0.0 404,625 + +0.5 404,625 + -2.3 404,627 + -1.9 404,627 + +1.0 404,627 + +1.6 404,628 - +0.3 404,628 - +0.2 404,628 - -2.1 404,631 + -0.4 404,632 - +1.5 404,632 - -2.0 404,792 + EX28DRAFT_0425 0.21 -0.4 404,792 + EX28DRAFT_0425 0.21 +1.0 405,023 + EX28DRAFT_0425 0.60 +0.6 405,078 + EX28DRAFT_0425 0.70 -0.5 405,079 - EX28DRAFT_0425 0.70 -0.3 405,079 - EX28DRAFT_0425 0.70 +0.0 405,091 + EX28DRAFT_0425 0.72 -1.0 405,092 - EX28DRAFT_0425 0.72 +0.7 405,092 - EX28DRAFT_0425 0.72 -0.5 405,109 + EX28DRAFT_0425 0.75 +1.1 405,142 - EX28DRAFT_0425 0.81 -1.6 405,276 - +0.1 405,383 + -0.4 405,403 + +0.2 405,404 - -0.1 405,450 - -0.7 405,578 + EX28DRAFT_0426 0.17 +0.0 405,588 + EX28DRAFT_0426 0.18 -0.3 405,589 - EX28DRAFT_0426 0.18 -0.0 405,637 + EX28DRAFT_0426 0.23 -0.1 405,730 - EX28DRAFT_0426 0.33 +0.4 405,906 + EX28DRAFT_0426 0.52 +1.6 405,907 - EX28DRAFT_0426 0.52 +1.3 406,052 + EX28DRAFT_0426 0.68 +0.1 406,053 - EX28DRAFT_0426 0.68 -0.3 406,053 - EX28DRAFT_0426 0.68 +0.1 406,053 - EX28DRAFT_0426 0.68 +0.9 406,053 - EX28DRAFT_0426 0.68 +1.1 406,053 - EX28DRAFT_0426 0.68 -4.4 406,053 - EX28DRAFT_0426 0.68 +0.3 406,074 - EX28DRAFT_0426 0.70 +0.7 406,084 + EX28DRAFT_0426 0.72 +0.0 406,086 + EX28DRAFT_0426 0.72 -0.6 406,176 - EX28DRAFT_0426 0.81 -3.0 406,215 + EX28DRAFT_0426 0.86 -0.3 406,215 + EX28DRAFT_0426 0.86 +0.1 406,215 + EX28DRAFT_0426 0.86 +0.4 406,215 + EX28DRAFT_0426 0.86 +0.5 406,215 + EX28DRAFT_0426 0.86 +0.6 406,216 - EX28DRAFT_0426 0.86 -0.6 406,247 + EX28DRAFT_0426 0.89 -0.3 406,248 - EX28DRAFT_0426 0.89 -0.4
Or see this region's nucleotide sequence