Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0334 and EX28DRAFT_0335 are separated by 92 nucleotides EX28DRAFT_0335 and EX28DRAFT_0336 are separated by 64 nucleotides
EX28DRAFT_0334: EX28DRAFT_0334 - proton-translocating NADH-quinone oxidoreductase, chain N, at 296,101 to 297,558
_0334
EX28DRAFT_0335: EX28DRAFT_0335 - Chemotaxis signal transduction protein, at 297,651 to 298,655
_0335
EX28DRAFT_0336: EX28DRAFT_0336 - ribonuclease Z, at 298,720 to 299,637
_0336
Position (kb) 297
298
299 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 297.504 kb on + strand at 297.508 kb on + strand at 297.652 kb on + strand at 297.652 kb on + strand at 297.653 kb on - strand at 298.007 kb on + strand, within EX28DRAFT_0335 at 298.007 kb on + strand, within EX28DRAFT_0335 at 298.171 kb on + strand, within EX28DRAFT_0335 at 298.172 kb on - strand, within EX28DRAFT_0335 at 298.172 kb on - strand, within EX28DRAFT_0335 at 298.252 kb on + strand, within EX28DRAFT_0335 at 298.252 kb on + strand, within EX28DRAFT_0335 at 298.253 kb on - strand, within EX28DRAFT_0335 at 298.395 kb on - strand, within EX28DRAFT_0335 at 298.523 kb on + strand, within EX28DRAFT_0335 at 298.635 kb on - strand at 298.635 kb on - strand at 298.649 kb on + strand at 298.658 kb on + strand at 298.722 kb on - strand at 298.722 kb on - strand at 299.048 kb on + strand, within EX28DRAFT_0336 at 299.175 kb on + strand, within EX28DRAFT_0336 at 299.201 kb on + strand, within EX28DRAFT_0336 at 299.323 kb on + strand, within EX28DRAFT_0336 at 299.329 kb on - strand, within EX28DRAFT_0336 at 299.560 kb on - strand at 299.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 297,504 + +0.2 297,508 + -1.9 297,652 + +2.0 297,652 + -1.6 297,653 - +0.4 298,007 + EX28DRAFT_0335 0.35 +0.1 298,007 + EX28DRAFT_0335 0.35 +1.3 298,171 + EX28DRAFT_0335 0.52 +1.9 298,172 - EX28DRAFT_0335 0.52 +0.0 298,172 - EX28DRAFT_0335 0.52 -2.0 298,252 + EX28DRAFT_0335 0.60 -0.9 298,252 + EX28DRAFT_0335 0.60 +0.0 298,253 - EX28DRAFT_0335 0.60 -1.5 298,395 - EX28DRAFT_0335 0.74 -0.4 298,523 + EX28DRAFT_0335 0.87 +0.5 298,635 - +0.5 298,635 - -3.1 298,649 + +0.6 298,658 + +1.6 298,722 - +0.6 298,722 - -0.9 299,048 + EX28DRAFT_0336 0.36 +0.8 299,175 + EX28DRAFT_0336 0.50 -0.1 299,201 + EX28DRAFT_0336 0.52 +0.6 299,323 + EX28DRAFT_0336 0.66 -0.1 299,329 - EX28DRAFT_0336 0.66 -0.2 299,560 - +2.5 299,617 - -0.7
Or see this region's nucleotide sequence