Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0186 and EX28DRAFT_0187 are separated by 73 nucleotides EX28DRAFT_0187 and EX28DRAFT_0188 are separated by 38 nucleotides EX28DRAFT_0188 and EX28DRAFT_0189 are separated by 24 nucleotides
EX28DRAFT_0186: EX28DRAFT_0186 - Predicted P-loop ATPase fused to an acetyltransferase, at 135,308 to 137,254
_0186
EX28DRAFT_0187: EX28DRAFT_0187 - Predicted esterase, at 137,328 to 138,023
_0187
EX28DRAFT_0188: EX28DRAFT_0188 - Uncharacterised protein family (UPF0370), at 138,062 to 138,259
_0188
EX28DRAFT_0189: EX28DRAFT_0189 - succinyldiaminopimelate desuccinylase (EC 3.5.1.18), at 138,284 to 139,411
_0189
Position (kb) 137
138
139 Strain fitness (log2 ratio) -2
-1
0
1 at 136.384 kb on - strand, within EX28DRAFT_0186 at 136.384 kb on - strand, within EX28DRAFT_0186 at 136.385 kb on + strand, within EX28DRAFT_0186 at 136.665 kb on + strand, within EX28DRAFT_0186 at 136.665 kb on + strand, within EX28DRAFT_0186 at 136.665 kb on + strand, within EX28DRAFT_0186 at 136.753 kb on + strand, within EX28DRAFT_0186 at 136.839 kb on - strand, within EX28DRAFT_0186 at 137.264 kb on + strand at 137.312 kb on + strand at 137.312 kb on + strand at 137.312 kb on + strand at 137.313 kb on - strand at 137.380 kb on + strand at 137.402 kb on - strand, within EX28DRAFT_0187 at 137.421 kb on - strand, within EX28DRAFT_0187 at 137.913 kb on - strand, within EX28DRAFT_0187 at 137.962 kb on - strand at 137.962 kb on - strand at 138.017 kb on + strand at 138.063 kb on + strand at 138.241 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 136,384 - EX28DRAFT_0186 0.55 +0.6 136,384 - EX28DRAFT_0186 0.55 -0.8 136,385 + EX28DRAFT_0186 0.55 +1.2 136,665 + EX28DRAFT_0186 0.70 -1.9 136,665 + EX28DRAFT_0186 0.70 +0.1 136,665 + EX28DRAFT_0186 0.70 +1.7 136,753 + EX28DRAFT_0186 0.74 +0.2 136,839 - EX28DRAFT_0186 0.79 +0.8 137,264 + +0.2 137,312 + -0.8 137,312 + +0.2 137,312 + +0.8 137,313 - -0.1 137,380 + +1.3 137,402 - EX28DRAFT_0187 0.11 -1.2 137,421 - EX28DRAFT_0187 0.13 -1.1 137,913 - EX28DRAFT_0187 0.84 -0.5 137,962 - -0.9 137,962 - -0.0 138,017 + -2.7 138,063 + -0.3 138,241 - +0.0
Or see this region's nucleotide sequence