Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0086 and EX28DRAFT_0087 are separated by 11 nucleotides EX28DRAFT_0087 and EX28DRAFT_0088 are separated by 3 nucleotides EX28DRAFT_0088 and EX28DRAFT_0089 are separated by 24 nucleotides
EX28DRAFT_0086: EX28DRAFT_0086 - N-acetylmuramic acid 6-phosphate etherase, at 25,217 to 26,110
_0086
EX28DRAFT_0087: EX28DRAFT_0087 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 26,122 to 27,483
_0087
EX28DRAFT_0088: EX28DRAFT_0088 - haloacid dehalogenase superfamily, subfamily IF hydrolase, YfhB, at 27,487 to 28,122
_0088
EX28DRAFT_0089: EX28DRAFT_0089 - Cytosine/adenosine deaminases, at 28,147 to 28,686
_0089
Position (kb) 26
27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 25.319 kb on - strand, within EX28DRAFT_0086 at 25.416 kb on - strand, within EX28DRAFT_0086 at 25.416 kb on - strand, within EX28DRAFT_0086 at 25.416 kb on - strand, within EX28DRAFT_0086 at 25.598 kb on - strand, within EX28DRAFT_0086 at 25.652 kb on + strand, within EX28DRAFT_0086 at 25.653 kb on - strand, within EX28DRAFT_0086 at 25.834 kb on + strand, within EX28DRAFT_0086 at 25.874 kb on + strand, within EX28DRAFT_0086 at 26.108 kb on + strand at 26.121 kb on + strand at 26.431 kb on + strand, within EX28DRAFT_0087 at 26.499 kb on + strand, within EX28DRAFT_0087 at 26.576 kb on - strand, within EX28DRAFT_0087 at 27.088 kb on + strand, within EX28DRAFT_0087 at 27.090 kb on + strand, within EX28DRAFT_0087 at 27.315 kb on + strand, within EX28DRAFT_0087 at 27.316 kb on - strand, within EX28DRAFT_0087 at 27.316 kb on - strand, within EX28DRAFT_0087 at 27.319 kb on + strand, within EX28DRAFT_0087 at 27.403 kb on + strand at 27.403 kb on + strand at 27.404 kb on - strand at 27.410 kb on + strand at 27.416 kb on - strand at 27.420 kb on - strand at 27.420 kb on - strand at 27.420 kb on - strand at 27.420 kb on - strand at 27.482 kb on - strand at 27.549 kb on + strand at 27.549 kb on + strand at 27.549 kb on + strand at 27.550 kb on - strand at 27.621 kb on - strand, within EX28DRAFT_0088 at 27.696 kb on + strand, within EX28DRAFT_0088 at 27.696 kb on + strand, within EX28DRAFT_0088 at 27.696 kb on + strand, within EX28DRAFT_0088 at 27.696 kb on + strand, within EX28DRAFT_0088 at 27.697 kb on - strand, within EX28DRAFT_0088 at 27.805 kb on + strand, within EX28DRAFT_0088 at 28.120 kb on + strand at 28.131 kb on - strand at 28.131 kb on - strand at 28.143 kb on + strand at 28.144 kb on - strand at 28.144 kb on - strand at 28.146 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 25,319 - EX28DRAFT_0086 0.11 +1.0 25,416 - EX28DRAFT_0086 0.22 -3.7 25,416 - EX28DRAFT_0086 0.22 +0.2 25,416 - EX28DRAFT_0086 0.22 +1.4 25,598 - EX28DRAFT_0086 0.43 -0.2 25,652 + EX28DRAFT_0086 0.49 -0.5 25,653 - EX28DRAFT_0086 0.49 -0.8 25,834 + EX28DRAFT_0086 0.69 -0.1 25,874 + EX28DRAFT_0086 0.73 +0.3 26,108 + -0.8 26,121 + +0.4 26,431 + EX28DRAFT_0087 0.23 -0.4 26,499 + EX28DRAFT_0087 0.28 -1.8 26,576 - EX28DRAFT_0087 0.33 +0.2 27,088 + EX28DRAFT_0087 0.71 -0.4 27,090 + EX28DRAFT_0087 0.71 +1.6 27,315 + EX28DRAFT_0087 0.88 -0.3 27,316 - EX28DRAFT_0087 0.88 +0.6 27,316 - EX28DRAFT_0087 0.88 +1.9 27,319 + EX28DRAFT_0087 0.88 +0.1 27,403 + -1.2 27,403 + -0.0 27,404 - +1.8 27,410 + -0.6 27,416 - -0.5 27,420 - -0.3 27,420 - +0.3 27,420 - -1.4 27,420 - -0.2 27,482 - +1.0 27,549 + +0.9 27,549 + +0.2 27,549 + +0.3 27,550 - +0.1 27,621 - EX28DRAFT_0088 0.21 -1.3 27,696 + EX28DRAFT_0088 0.33 -0.5 27,696 + EX28DRAFT_0088 0.33 -0.5 27,696 + EX28DRAFT_0088 0.33 -1.6 27,696 + EX28DRAFT_0088 0.33 +0.5 27,697 - EX28DRAFT_0088 0.33 +0.3 27,805 + EX28DRAFT_0088 0.50 +0.4 28,120 + -0.7 28,131 - +0.2 28,131 - -0.4 28,143 + +0.5 28,144 - -0.8 28,144 - +0.4 28,146 - -0.4
Or see this region's nucleotide sequence