Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0060 and EX28DRAFT_0061 overlap by 4 nucleotides EX28DRAFT_0061 and EX28DRAFT_0062 are separated by 45 nucleotides EX28DRAFT_0062 and EX28DRAFT_0063 are separated by 110 nucleotides
EX28DRAFT_0060: EX28DRAFT_0060 - metabolite-proton symporter, at 521 to 1,816
_0060
EX28DRAFT_0061: EX28DRAFT_0061 - Predicted periplasmic lipoprotein, at 1,813 to 2,136
_0061
EX28DRAFT_0062: EX28DRAFT_0062 - Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes, at 2,182 to 3,537
_0062
EX28DRAFT_0063: EX28DRAFT_0063 - Acyl-CoA synthetase (NDP forming), at 3,648 to 6,311
_0063
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1.256 kb on - strand, within EX28DRAFT_0060 at 1.310 kb on + strand, within EX28DRAFT_0060 at 1.310 kb on + strand, within EX28DRAFT_0060 at 1.311 kb on - strand, within EX28DRAFT_0060 at 1.311 kb on - strand, within EX28DRAFT_0060 at 1.311 kb on - strand, within EX28DRAFT_0060 at 1.311 kb on - strand, within EX28DRAFT_0060 at 1.311 kb on - strand, within EX28DRAFT_0060 at 1.312 kb on + strand, within EX28DRAFT_0060 at 1.393 kb on + strand, within EX28DRAFT_0060 at 1.401 kb on + strand, within EX28DRAFT_0060 at 1.401 kb on + strand, within EX28DRAFT_0060 at 1.408 kb on + strand, within EX28DRAFT_0060 at 1.408 kb on + strand, within EX28DRAFT_0060 at 1.408 kb on + strand, within EX28DRAFT_0060 at 1.409 kb on - strand, within EX28DRAFT_0060 at 1.409 kb on - strand, within EX28DRAFT_0060 at 1.409 kb on - strand, within EX28DRAFT_0060 at 1.409 kb on - strand, within EX28DRAFT_0060 at 1.445 kb on - strand, within EX28DRAFT_0060 at 1.480 kb on - strand, within EX28DRAFT_0060 at 1.483 kb on + strand, within EX28DRAFT_0060 at 1.483 kb on + strand, within EX28DRAFT_0060 at 1.483 kb on + strand, within EX28DRAFT_0060 at 1.492 kb on + strand, within EX28DRAFT_0060 at 1.493 kb on - strand, within EX28DRAFT_0060 at 1.493 kb on - strand, within EX28DRAFT_0060 at 1.574 kb on + strand, within EX28DRAFT_0060 at 1.574 kb on + strand, within EX28DRAFT_0060 at 1.575 kb on - strand, within EX28DRAFT_0060 at 1.582 kb on + strand, within EX28DRAFT_0060 at 1.583 kb on - strand, within EX28DRAFT_0060 at 1.583 kb on - strand, within EX28DRAFT_0060 at 1.583 kb on - strand, within EX28DRAFT_0060 at 1.623 kb on + strand, within EX28DRAFT_0060 at 1.623 kb on + strand, within EX28DRAFT_0060 at 1.624 kb on - strand, within EX28DRAFT_0060 at 1.650 kb on - strand, within EX28DRAFT_0060 at 1.660 kb on + strand, within EX28DRAFT_0060 at 1.661 kb on - strand, within EX28DRAFT_0060 at 1.661 kb on - strand, within EX28DRAFT_0060 at 1.661 kb on - strand, within EX28DRAFT_0060 at 1.972 kb on - strand, within EX28DRAFT_0061 at 1.985 kb on + strand, within EX28DRAFT_0061 at 2.074 kb on - strand, within EX28DRAFT_0061 at 2.119 kb on + strand at 2.130 kb on + strand at 2.131 kb on - strand at 3.578 kb on - strand at 3.644 kb on + strand at 3.649 kb on + strand at 3.696 kb on - strand at 3.765 kb on - strand at 3.770 kb on + strand at 3.771 kb on - strand at 3.771 kb on - strand at 3.771 kb on - strand at 3.774 kb on - strand at 3.810 kb on - strand at 3.811 kb on + strand at 3.812 kb on - strand at 4.058 kb on - strand, within EX28DRAFT_0063
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,256 - EX28DRAFT_0060 0.57 +0.2 1,310 + EX28DRAFT_0060 0.61 +0.0 1,310 + EX28DRAFT_0060 0.61 +0.3 1,311 - EX28DRAFT_0060 0.61 +0.0 1,311 - EX28DRAFT_0060 0.61 +0.6 1,311 - EX28DRAFT_0060 0.61 -3.8 1,311 - EX28DRAFT_0060 0.61 -0.1 1,311 - EX28DRAFT_0060 0.61 +0.3 1,312 + EX28DRAFT_0060 0.61 +0.6 1,393 + EX28DRAFT_0060 0.67 +0.9 1,401 + EX28DRAFT_0060 0.68 -0.9 1,401 + EX28DRAFT_0060 0.68 -0.2 1,408 + EX28DRAFT_0060 0.68 -0.1 1,408 + EX28DRAFT_0060 0.68 -1.0 1,408 + EX28DRAFT_0060 0.68 +0.1 1,409 - EX28DRAFT_0060 0.69 -0.1 1,409 - EX28DRAFT_0060 0.69 -0.1 1,409 - EX28DRAFT_0060 0.69 -0.6 1,409 - EX28DRAFT_0060 0.69 +0.7 1,445 - EX28DRAFT_0060 0.71 +0.5 1,480 - EX28DRAFT_0060 0.74 +0.8 1,483 + EX28DRAFT_0060 0.74 +1.3 1,483 + EX28DRAFT_0060 0.74 +0.5 1,483 + EX28DRAFT_0060 0.74 -0.5 1,492 + EX28DRAFT_0060 0.75 +1.6 1,493 - EX28DRAFT_0060 0.75 +0.6 1,493 - EX28DRAFT_0060 0.75 -2.4 1,574 + EX28DRAFT_0060 0.81 -3.3 1,574 + EX28DRAFT_0060 0.81 +0.4 1,575 - EX28DRAFT_0060 0.81 -1.0 1,582 + EX28DRAFT_0060 0.82 +1.1 1,583 - EX28DRAFT_0060 0.82 +0.9 1,583 - EX28DRAFT_0060 0.82 +0.1 1,583 - EX28DRAFT_0060 0.82 +0.4 1,623 + EX28DRAFT_0060 0.85 +0.9 1,623 + EX28DRAFT_0060 0.85 +0.4 1,624 - EX28DRAFT_0060 0.85 -0.2 1,650 - EX28DRAFT_0060 0.87 +0.3 1,660 + EX28DRAFT_0060 0.88 -0.1 1,661 - EX28DRAFT_0060 0.88 +0.5 1,661 - EX28DRAFT_0060 0.88 +0.3 1,661 - EX28DRAFT_0060 0.88 -0.4 1,972 - EX28DRAFT_0061 0.49 +0.8 1,985 + EX28DRAFT_0061 0.53 +0.4 2,074 - EX28DRAFT_0061 0.81 -0.8 2,119 + -0.8 2,130 + -1.1 2,131 - -1.3 3,578 - +1.6 3,644 + +0.2 3,649 + +0.2 3,696 - -1.0 3,765 - -0.3 3,770 + +0.7 3,771 - +0.5 3,771 - -0.1 3,771 - -0.5 3,774 - -0.4 3,810 - -0.0 3,811 + -0.2 3,812 - -0.6 4,058 - EX28DRAFT_0063 0.15 -1.0
Or see this region's nucleotide sequence