Experiment: without_iron2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20655 and AMB_RS20660 are separated by 89 nucleotides AMB_RS20660 and AMB_RS20665 overlap by 4 nucleotides AMB_RS20665 and AMB_RS20670 overlap by 4 nucleotides AMB_RS20670 and AMB_RS20675 are separated by 145 nucleotides
AMB_RS20655: AMB_RS20655 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 4,486,804 to 4,487,262
_RS20655
AMB_RS20660: AMB_RS20660 - hypothetical protein, at 4,487,352 to 4,487,867
_RS20660
AMB_RS20665: AMB_RS20665 - class I SAM-dependent methyltransferase, at 4,487,864 to 4,488,562
_RS20665
AMB_RS20670: AMB_RS20670 - tRNA (cytidine(34)-2'-O)-methyltransferase, at 4,488,559 to 4,489,050
_RS20670
AMB_RS20675: AMB_RS20675 - ubiquinol-cytochrome c reductase iron-sulfur subunit, at 4,489,196 to 4,489,750
_RS20675
Position (kb)
4487
4488
4489 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4486.870 kb on + strand, within AMB_RS20655 at 4486.870 kb on + strand, within AMB_RS20655 at 4486.871 kb on - strand, within AMB_RS20655 at 4486.871 kb on - strand, within AMB_RS20655 at 4487.249 kb on - strand at 4487.369 kb on + strand at 4487.370 kb on - strand at 4487.403 kb on + strand at 4487.403 kb on + strand at 4487.404 kb on - strand, within AMB_RS20660 at 4487.404 kb on - strand, within AMB_RS20660 at 4487.404 kb on - strand, within AMB_RS20660 at 4487.511 kb on - strand, within AMB_RS20660 at 4487.556 kb on + strand, within AMB_RS20660 at 4487.556 kb on + strand, within AMB_RS20660 at 4487.557 kb on - strand, within AMB_RS20660 at 4487.614 kb on - strand, within AMB_RS20660 at 4487.865 kb on + strand at 4487.865 kb on + strand at 4487.865 kb on + strand at 4487.866 kb on - strand at 4487.866 kb on - strand at 4487.866 kb on - strand at 4487.866 kb on - strand at 4487.866 kb on - strand at 4487.911 kb on - strand at 4488.261 kb on + strand, within AMB_RS20665 at 4488.262 kb on - strand, within AMB_RS20665 at 4488.262 kb on - strand, within AMB_RS20665 at 4488.481 kb on - strand, within AMB_RS20665 at 4488.514 kb on - strand at 4488.514 kb on - strand at 4488.858 kb on + strand, within AMB_RS20670 at 4488.858 kb on + strand, within AMB_RS20670 at 4488.859 kb on - strand, within AMB_RS20670 at 4488.869 kb on + strand, within AMB_RS20670 at 4488.869 kb on + strand, within AMB_RS20670 at 4488.870 kb on - strand, within AMB_RS20670 at 4488.870 kb on - strand, within AMB_RS20670 at 4488.870 kb on - strand, within AMB_RS20670 at 4488.885 kb on + strand, within AMB_RS20670 at 4488.885 kb on + strand, within AMB_RS20670 at 4488.885 kb on + strand, within AMB_RS20670 at 4488.885 kb on + strand, within AMB_RS20670 at 4488.885 kb on + strand, within AMB_RS20670 at 4488.885 kb on + strand, within AMB_RS20670 at 4488.885 kb on + strand, within AMB_RS20670 at 4488.886 kb on - strand, within AMB_RS20670 at 4488.886 kb on - strand, within AMB_RS20670 at 4488.886 kb on - strand, within AMB_RS20670 at 4488.887 kb on + strand, within AMB_RS20670 at 4488.887 kb on + strand, within AMB_RS20670 at 4488.888 kb on - strand, within AMB_RS20670 at 4488.888 kb on - strand, within AMB_RS20670 at 4488.888 kb on - strand, within AMB_RS20670 at 4488.888 kb on - strand, within AMB_RS20670 at 4488.888 kb on - strand, within AMB_RS20670 at 4488.913 kb on - strand, within AMB_RS20670 at 4488.913 kb on - strand, within AMB_RS20670 at 4488.947 kb on - strand, within AMB_RS20670 at 4488.948 kb on + strand, within AMB_RS20670 at 4488.948 kb on + strand, within AMB_RS20670 at 4488.948 kb on + strand, within AMB_RS20670 at 4489.019 kb on + strand at 4489.019 kb on + strand at 4489.157 kb on + strand at 4489.157 kb on + strand at 4489.157 kb on + strand at 4489.157 kb on + strand at 4489.160 kb on + strand at 4489.160 kb on + strand at 4489.160 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction without_iron2 remove 4,486,870 + AMB_RS20655 0.14 +0.9 4,486,870 + AMB_RS20655 0.14 +0.7 4,486,871 - AMB_RS20655 0.15 -0.0 4,486,871 - AMB_RS20655 0.15 -0.2 4,487,249 - +0.1 4,487,369 + -0.5 4,487,370 - +0.9 4,487,403 + -0.0 4,487,403 + -1.0 4,487,404 - AMB_RS20660 0.10 +0.3 4,487,404 - AMB_RS20660 0.10 +2.5 4,487,404 - AMB_RS20660 0.10 -0.5 4,487,511 - AMB_RS20660 0.31 -0.2 4,487,556 + AMB_RS20660 0.40 +1.2 4,487,556 + AMB_RS20660 0.40 -0.7 4,487,557 - AMB_RS20660 0.40 +0.7 4,487,614 - AMB_RS20660 0.51 -0.6 4,487,865 + -1.1 4,487,865 + +0.5 4,487,865 + -0.2 4,487,866 - -0.9 4,487,866 - +0.3 4,487,866 - +0.1 4,487,866 - -0.4 4,487,866 - -0.3 4,487,911 - +0.7 4,488,261 + AMB_RS20665 0.57 -3.0 4,488,262 - AMB_RS20665 0.57 +0.4 4,488,262 - AMB_RS20665 0.57 -0.9 4,488,481 - AMB_RS20665 0.88 -0.8 4,488,514 - +1.1 4,488,514 - -0.2 4,488,858 + AMB_RS20670 0.61 -0.3 4,488,858 + AMB_RS20670 0.61 +2.0 4,488,859 - AMB_RS20670 0.61 -1.7 4,488,869 + AMB_RS20670 0.63 -0.9 4,488,869 + AMB_RS20670 0.63 -1.0 4,488,870 - AMB_RS20670 0.63 -0.5 4,488,870 - AMB_RS20670 0.63 -0.3 4,488,870 - AMB_RS20670 0.63 +0.1 4,488,885 + AMB_RS20670 0.66 +0.3 4,488,885 + AMB_RS20670 0.66 +0.2 4,488,885 + AMB_RS20670 0.66 -0.3 4,488,885 + AMB_RS20670 0.66 -0.1 4,488,885 + AMB_RS20670 0.66 +0.2 4,488,885 + AMB_RS20670 0.66 +0.1 4,488,885 + AMB_RS20670 0.66 +0.3 4,488,886 - AMB_RS20670 0.66 -0.4 4,488,886 - AMB_RS20670 0.66 -0.2 4,488,886 - AMB_RS20670 0.66 -0.4 4,488,887 + AMB_RS20670 0.67 -0.1 4,488,887 + AMB_RS20670 0.67 -1.0 4,488,888 - AMB_RS20670 0.67 +0.8 4,488,888 - AMB_RS20670 0.67 +0.0 4,488,888 - AMB_RS20670 0.67 +0.5 4,488,888 - AMB_RS20670 0.67 +0.1 4,488,888 - AMB_RS20670 0.67 +0.2 4,488,913 - AMB_RS20670 0.72 -1.2 4,488,913 - AMB_RS20670 0.72 +0.5 4,488,947 - AMB_RS20670 0.79 +0.0 4,488,948 + AMB_RS20670 0.79 -0.1 4,488,948 + AMB_RS20670 0.79 +0.0 4,488,948 + AMB_RS20670 0.79 +0.4 4,489,019 + -1.8 4,489,019 + +0.7 4,489,157 + +0.2 4,489,157 + +0.1 4,489,157 + +0.9 4,489,157 + -0.4 4,489,160 + -0.2 4,489,160 + +1.9 4,489,160 + -2.6
Or see this region's nucleotide sequence