Experiment: without_iron2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS15195 and AMB_RS15200 are separated by 88 nucleotides AMB_RS15200 and AMB_RS15205 are separated by 64 nucleotides AMB_RS15205 and AMB_RS15210 are separated by 43 nucleotides
AMB_RS15195: AMB_RS15195 - cytochrome c-type protein nrfB precursor, at 3,260,004 to 3,260,960
_RS15195
AMB_RS15200: AMB_RS15200 - hypothetical protein, at 3,261,049 to 3,263,244
_RS15200
AMB_RS15205: AMB_RS15205 - tRNA-Met, at 3,263,309 to 3,263,385
_RS15205
AMB_RS15210: AMB_RS15210 - tRNA 2-thiouridine(34) synthase MnmA, at 3,263,429 to 3,264,550
_RS15210
Position (kb)
3261
3262
3263
3264 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3260.649 kb on + strand, within AMB_RS15195 at 3260.649 kb on + strand, within AMB_RS15195 at 3260.650 kb on - strand, within AMB_RS15195 at 3260.871 kb on + strand at 3260.871 kb on + strand at 3260.871 kb on + strand at 3260.871 kb on + strand at 3260.871 kb on + strand at 3260.871 kb on + strand at 3261.326 kb on - strand, within AMB_RS15200 at 3261.326 kb on - strand, within AMB_RS15200 at 3261.470 kb on - strand, within AMB_RS15200 at 3261.470 kb on - strand, within AMB_RS15200 at 3261.470 kb on - strand, within AMB_RS15200 at 3261.470 kb on - strand, within AMB_RS15200 at 3261.470 kb on - strand, within AMB_RS15200 at 3261.470 kb on - strand, within AMB_RS15200 at 3261.574 kb on + strand, within AMB_RS15200 at 3261.575 kb on - strand, within AMB_RS15200 at 3261.631 kb on + strand, within AMB_RS15200 at 3261.631 kb on + strand, within AMB_RS15200 at 3261.631 kb on + strand, within AMB_RS15200 at 3261.632 kb on - strand, within AMB_RS15200 at 3261.757 kb on + strand, within AMB_RS15200 at 3261.757 kb on + strand, within AMB_RS15200 at 3261.790 kb on + strand, within AMB_RS15200 at 3261.791 kb on - strand, within AMB_RS15200 at 3261.824 kb on - strand, within AMB_RS15200 at 3261.859 kb on + strand, within AMB_RS15200 at 3261.859 kb on + strand, within AMB_RS15200 at 3261.859 kb on + strand, within AMB_RS15200 at 3262.009 kb on + strand, within AMB_RS15200 at 3262.051 kb on + strand, within AMB_RS15200 at 3262.084 kb on + strand, within AMB_RS15200 at 3262.084 kb on + strand, within AMB_RS15200 at 3262.084 kb on + strand, within AMB_RS15200 at 3262.084 kb on + strand, within AMB_RS15200 at 3262.085 kb on - strand, within AMB_RS15200 at 3262.085 kb on - strand, within AMB_RS15200 at 3262.085 kb on - strand, within AMB_RS15200 at 3262.166 kb on - strand, within AMB_RS15200 at 3262.202 kb on - strand, within AMB_RS15200 at 3262.202 kb on - strand, within AMB_RS15200 at 3262.415 kb on - strand, within AMB_RS15200 at 3262.685 kb on - strand, within AMB_RS15200 at 3262.769 kb on - strand, within AMB_RS15200 at 3262.774 kb on + strand, within AMB_RS15200 at 3262.775 kb on - strand, within AMB_RS15200 at 3262.775 kb on - strand, within AMB_RS15200 at 3262.775 kb on - strand, within AMB_RS15200 at 3262.775 kb on - strand, within AMB_RS15200 at 3263.105 kb on - strand at 3263.105 kb on - strand at 3263.119 kb on + strand at 3263.120 kb on - strand at 3263.125 kb on + strand at 3263.125 kb on + strand at 3263.125 kb on + strand at 3263.126 kb on - strand at 3263.200 kb on + strand at 3263.201 kb on - strand at 3263.242 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction without_iron2 remove 3,260,649 + AMB_RS15195 0.67 -2.0 3,260,649 + AMB_RS15195 0.67 -0.3 3,260,650 - AMB_RS15195 0.68 -0.4 3,260,871 + +0.5 3,260,871 + +0.2 3,260,871 + -0.5 3,260,871 + -1.7 3,260,871 + -0.6 3,260,871 + -0.6 3,261,326 - AMB_RS15200 0.13 +0.4 3,261,326 - AMB_RS15200 0.13 -2.6 3,261,470 - AMB_RS15200 0.19 -0.0 3,261,470 - AMB_RS15200 0.19 -0.9 3,261,470 - AMB_RS15200 0.19 -1.2 3,261,470 - AMB_RS15200 0.19 -1.2 3,261,470 - AMB_RS15200 0.19 +0.4 3,261,470 - AMB_RS15200 0.19 -1.6 3,261,574 + AMB_RS15200 0.24 -0.4 3,261,575 - AMB_RS15200 0.24 -0.2 3,261,631 + AMB_RS15200 0.27 +0.4 3,261,631 + AMB_RS15200 0.27 -0.3 3,261,631 + AMB_RS15200 0.27 -1.0 3,261,632 - AMB_RS15200 0.27 -0.3 3,261,757 + AMB_RS15200 0.32 -2.1 3,261,757 + AMB_RS15200 0.32 +0.8 3,261,790 + AMB_RS15200 0.34 -0.7 3,261,791 - AMB_RS15200 0.34 -1.2 3,261,824 - AMB_RS15200 0.35 -1.9 3,261,859 + AMB_RS15200 0.37 -0.2 3,261,859 + AMB_RS15200 0.37 -0.8 3,261,859 + AMB_RS15200 0.37 -2.2 3,262,009 + AMB_RS15200 0.44 -0.3 3,262,051 + AMB_RS15200 0.46 -1.5 3,262,084 + AMB_RS15200 0.47 -3.1 3,262,084 + AMB_RS15200 0.47 -1.5 3,262,084 + AMB_RS15200 0.47 -2.1 3,262,084 + AMB_RS15200 0.47 -1.3 3,262,085 - AMB_RS15200 0.47 -1.9 3,262,085 - AMB_RS15200 0.47 -0.0 3,262,085 - AMB_RS15200 0.47 -3.1 3,262,166 - AMB_RS15200 0.51 -0.7 3,262,202 - AMB_RS15200 0.53 -0.9 3,262,202 - AMB_RS15200 0.53 -1.2 3,262,415 - AMB_RS15200 0.62 +2.0 3,262,685 - AMB_RS15200 0.74 -0.2 3,262,769 - AMB_RS15200 0.78 -2.2 3,262,774 + AMB_RS15200 0.79 -2.3 3,262,775 - AMB_RS15200 0.79 -1.2 3,262,775 - AMB_RS15200 0.79 -0.8 3,262,775 - AMB_RS15200 0.79 +0.4 3,262,775 - AMB_RS15200 0.79 -1.3 3,263,105 - -1.3 3,263,105 - -1.6 3,263,119 + -0.9 3,263,120 - -0.2 3,263,125 + -0.2 3,263,125 + -0.9 3,263,125 + -2.1 3,263,126 - +0.2 3,263,200 + -1.9 3,263,201 - -0.8 3,263,242 + +0.7
Or see this region's nucleotide sequence