Experiment: without_iron2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS06655 and AMB_RS06660 are separated by 119 nucleotides AMB_RS06660 and AMB_RS06665 overlap by 12 nucleotides AMB_RS06665 and AMB_RS06670 overlap by 1 nucleotides AMB_RS06670 and AMB_RS06675 are separated by 27 nucleotides AMB_RS06675 and AMB_RS06680 are separated by 139 nucleotides
AMB_RS06655: AMB_RS06655 - spore maturation protein, at 1,427,437 to 1,428,672
_RS06655
AMB_RS06660: AMB_RS06660 - hypothetical protein, at 1,428,792 to 1,429,373
_RS06660
AMB_RS06665: AMB_RS06665 - LrgB family protein, at 1,429,362 to 1,430,081
_RS06665
AMB_RS06670: AMB_RS06670 - CidA/LrgA family protein, at 1,430,081 to 1,430,434
_RS06670
AMB_RS06675: AMB_RS06675 - membrane protein, at 1,430,462 to 1,430,746
_RS06675
AMB_RS06680: AMB_RS06680 - orotate phosphoribosyltransferase, at 1,430,886 to 1,431,578
_RS06680
Position (kb)
1429
1430
1431 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1428.704 kb on - strand at 1428.754 kb on - strand at 1428.754 kb on - strand at 1428.773 kb on - strand at 1428.809 kb on + strand at 1428.810 kb on - strand at 1429.077 kb on + strand, within AMB_RS06660 at 1429.077 kb on + strand, within AMB_RS06660 at 1429.077 kb on + strand, within AMB_RS06660 at 1429.078 kb on - strand, within AMB_RS06660 at 1429.078 kb on - strand, within AMB_RS06660 at 1429.078 kb on - strand, within AMB_RS06660 at 1429.078 kb on - strand, within AMB_RS06660 at 1429.372 kb on - strand at 1429.813 kb on + strand, within AMB_RS06665 at 1430.002 kb on + strand, within AMB_RS06665 at 1430.003 kb on - strand, within AMB_RS06665 at 1430.050 kb on + strand at 1430.050 kb on + strand at 1430.050 kb on + strand at 1430.050 kb on + strand at 1430.050 kb on + strand at 1430.050 kb on + strand at 1430.051 kb on - strand at 1430.099 kb on - strand at 1430.246 kb on + strand, within AMB_RS06670 at 1430.246 kb on + strand, within AMB_RS06670 at 1430.247 kb on - strand, within AMB_RS06670 at 1430.422 kb on + strand at 1430.422 kb on + strand at 1430.423 kb on - strand at 1430.423 kb on - strand at 1430.423 kb on - strand at 1430.423 kb on - strand at 1430.423 kb on - strand at 1430.423 kb on - strand at 1430.424 kb on + strand at 1430.424 kb on + strand at 1430.424 kb on + strand at 1430.425 kb on - strand at 1430.425 kb on - strand at 1430.491 kb on - strand, within AMB_RS06675 at 1430.513 kb on - strand, within AMB_RS06675 at 1430.550 kb on + strand, within AMB_RS06675 at 1430.550 kb on + strand, within AMB_RS06675 at 1430.574 kb on + strand, within AMB_RS06675 at 1430.574 kb on + strand, within AMB_RS06675 at 1430.574 kb on + strand, within AMB_RS06675 at 1430.575 kb on - strand, within AMB_RS06675 at 1430.575 kb on - strand, within AMB_RS06675 at 1430.575 kb on - strand, within AMB_RS06675 at 1430.575 kb on - strand, within AMB_RS06675 at 1430.575 kb on - strand, within AMB_RS06675 at 1430.575 kb on - strand, within AMB_RS06675 at 1430.575 kb on - strand, within AMB_RS06675 at 1430.575 kb on - strand, within AMB_RS06675 at 1430.575 kb on - strand, within AMB_RS06675 at 1430.575 kb on - strand, within AMB_RS06675 at 1430.627 kb on + strand, within AMB_RS06675 at 1430.628 kb on - strand, within AMB_RS06675 at 1430.800 kb on - strand at 1430.819 kb on + strand at 1430.819 kb on + strand at 1430.819 kb on + strand at 1430.819 kb on + strand at 1430.820 kb on - strand at 1430.820 kb on - strand at 1430.820 kb on - strand at 1430.821 kb on + strand at 1430.822 kb on - strand at 1430.822 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction without_iron2 remove 1,428,704 - -0.6 1,428,754 - +1.0 1,428,754 - -1.8 1,428,773 - -0.6 1,428,809 + -1.0 1,428,810 - -1.1 1,429,077 + AMB_RS06660 0.49 -0.6 1,429,077 + AMB_RS06660 0.49 +0.1 1,429,077 + AMB_RS06660 0.49 -0.9 1,429,078 - AMB_RS06660 0.49 -0.0 1,429,078 - AMB_RS06660 0.49 -0.1 1,429,078 - AMB_RS06660 0.49 -0.2 1,429,078 - AMB_RS06660 0.49 +1.4 1,429,372 - -1.1 1,429,813 + AMB_RS06665 0.63 -0.4 1,430,002 + AMB_RS06665 0.89 -1.3 1,430,003 - AMB_RS06665 0.89 -0.7 1,430,050 + -0.2 1,430,050 + -1.3 1,430,050 + -1.2 1,430,050 + +0.4 1,430,050 + -1.2 1,430,050 + -0.3 1,430,051 - -0.0 1,430,099 - -0.3 1,430,246 + AMB_RS06670 0.47 -0.3 1,430,246 + AMB_RS06670 0.47 -0.5 1,430,247 - AMB_RS06670 0.47 +0.9 1,430,422 + -1.1 1,430,422 + -0.0 1,430,423 - +1.5 1,430,423 - -0.4 1,430,423 - +0.2 1,430,423 - -0.4 1,430,423 - -0.3 1,430,423 - -0.0 1,430,424 + -0.1 1,430,424 + +0.4 1,430,424 + -0.3 1,430,425 - -0.3 1,430,425 - -0.2 1,430,491 - AMB_RS06675 0.10 +0.6 1,430,513 - AMB_RS06675 0.18 -0.2 1,430,550 + AMB_RS06675 0.31 -0.7 1,430,550 + AMB_RS06675 0.31 -0.9 1,430,574 + AMB_RS06675 0.39 -1.2 1,430,574 + AMB_RS06675 0.39 -0.1 1,430,574 + AMB_RS06675 0.39 -0.2 1,430,575 - AMB_RS06675 0.40 +0.7 1,430,575 - AMB_RS06675 0.40 -0.2 1,430,575 - AMB_RS06675 0.40 -2.8 1,430,575 - AMB_RS06675 0.40 +1.0 1,430,575 - AMB_RS06675 0.40 -0.6 1,430,575 - AMB_RS06675 0.40 +0.6 1,430,575 - AMB_RS06675 0.40 -0.6 1,430,575 - AMB_RS06675 0.40 +0.5 1,430,575 - AMB_RS06675 0.40 -0.5 1,430,575 - AMB_RS06675 0.40 +0.4 1,430,627 + AMB_RS06675 0.58 -0.2 1,430,628 - AMB_RS06675 0.58 +0.2 1,430,800 - +0.5 1,430,819 + -0.4 1,430,819 + -1.9 1,430,819 + +0.9 1,430,819 + -0.2 1,430,820 - +0.4 1,430,820 - -1.8 1,430,820 - +1.0 1,430,821 + -1.3 1,430,822 - -0.2 1,430,822 - +1.4
Or see this region's nucleotide sequence