Experiment: without_iron2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23945 and AMB_RS05070 are separated by 158 nucleotides AMB_RS05070 and AMB_RS05075 overlap by 11 nucleotides AMB_RS05075 and AMB_RS05080 are separated by 645 nucleotides
AMB_RS23945: AMB_RS23945 - DUF3365 domain-containing protein, at 1,047,347 to 1,049,650
_RS23945
AMB_RS05070: AMB_RS05070 - HU family DNA-binding protein, at 1,049,809 to 1,050,087
_RS05070
AMB_RS05075: AMB_RS05075 - transcriptional regulator, at 1,050,077 to 1,050,490
_RS05075
AMB_RS05080: AMB_RS05080 - response regulator, at 1,051,136 to 1,051,576
_RS05080
Position (kb)
1050
1051 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1049.295 kb on + strand, within AMB_RS23945 at 1049.295 kb on + strand, within AMB_RS23945 at 1049.647 kb on - strand at 1049.648 kb on + strand at 1049.648 kb on + strand at 1049.648 kb on + strand at 1049.649 kb on - strand at 1049.665 kb on - strand at 1049.717 kb on + strand at 1049.717 kb on + strand at 1049.717 kb on + strand at 1049.717 kb on + strand at 1049.717 kb on + strand at 1049.718 kb on - strand at 1049.718 kb on - strand at 1049.718 kb on - strand at 1049.718 kb on - strand at 1050.407 kb on + strand, within AMB_RS05075 at 1050.453 kb on - strand at 1050.453 kb on - strand at 1050.506 kb on + strand at 1050.507 kb on - strand at 1050.507 kb on - strand at 1050.507 kb on - strand at 1050.522 kb on + strand at 1050.522 kb on + strand at 1050.522 kb on + strand at 1050.522 kb on + strand at 1050.522 kb on + strand at 1050.522 kb on + strand at 1050.523 kb on - strand at 1050.523 kb on - strand at 1050.523 kb on - strand at 1050.523 kb on - strand at 1050.764 kb on - strand at 1050.876 kb on + strand at 1050.876 kb on + strand at 1050.876 kb on + strand at 1050.970 kb on + strand at 1050.970 kb on + strand at 1050.971 kb on - strand at 1050.971 kb on - strand at 1050.974 kb on + strand at 1050.974 kb on + strand at 1050.974 kb on + strand at 1050.974 kb on + strand at 1050.974 kb on + strand at 1050.974 kb on + strand at 1050.974 kb on + strand at 1050.975 kb on - strand at 1051.104 kb on + strand at 1051.104 kb on + strand at 1051.105 kb on - strand at 1051.105 kb on - strand at 1051.105 kb on - strand at 1051.105 kb on - strand at 1051.105 kb on - strand at 1051.106 kb on + strand at 1051.106 kb on + strand at 1051.107 kb on - strand at 1051.107 kb on - strand at 1051.107 kb on - strand at 1051.111 kb on + strand at 1051.111 kb on + strand at 1051.111 kb on + strand at 1051.112 kb on - strand at 1051.112 kb on - strand at 1051.252 kb on + strand, within AMB_RS05080 at 1051.252 kb on + strand, within AMB_RS05080 at 1051.252 kb on + strand, within AMB_RS05080 at 1051.253 kb on - strand, within AMB_RS05080 at 1051.253 kb on - strand, within AMB_RS05080 at 1051.363 kb on - strand, within AMB_RS05080 at 1051.363 kb on - strand, within AMB_RS05080 at 1051.363 kb on - strand, within AMB_RS05080 at 1051.363 kb on - strand, within AMB_RS05080 at 1051.373 kb on - strand, within AMB_RS05080 at 1051.373 kb on - strand, within AMB_RS05080 at 1051.405 kb on + strand, within AMB_RS05080
Per-strain Table
Position Strand Gene LocusTag Fraction without_iron2 remove 1,049,295 + AMB_RS23945 0.85 -0.5 1,049,295 + AMB_RS23945 0.85 -0.7 1,049,647 - +0.7 1,049,648 + +0.0 1,049,648 + +0.1 1,049,648 + -1.6 1,049,649 - +0.0 1,049,665 - -0.5 1,049,717 + -0.7 1,049,717 + -0.9 1,049,717 + +0.2 1,049,717 + -0.1 1,049,717 + -1.4 1,049,718 - +0.3 1,049,718 - -0.3 1,049,718 - -1.4 1,049,718 - -1.3 1,050,407 + AMB_RS05075 0.80 -0.8 1,050,453 - +0.0 1,050,453 - -1.0 1,050,506 + -0.9 1,050,507 - -1.4 1,050,507 - -1.0 1,050,507 - -0.6 1,050,522 + +0.2 1,050,522 + +0.2 1,050,522 + +0.4 1,050,522 + -0.8 1,050,522 + -0.2 1,050,522 + -0.8 1,050,523 - +1.2 1,050,523 - +0.4 1,050,523 - +0.3 1,050,523 - -0.5 1,050,764 - +0.7 1,050,876 + -0.9 1,050,876 + +0.4 1,050,876 + -0.4 1,050,970 + +0.4 1,050,970 + -0.7 1,050,971 - -0.7 1,050,971 - -0.0 1,050,974 + -0.2 1,050,974 + -0.0 1,050,974 + -0.9 1,050,974 + +1.2 1,050,974 + -0.2 1,050,974 + +0.1 1,050,974 + +0.4 1,050,975 - +0.0 1,051,104 + -1.4 1,051,104 + -1.0 1,051,105 - -1.6 1,051,105 - -2.1 1,051,105 - -0.1 1,051,105 - -0.5 1,051,105 - -0.4 1,051,106 + -0.5 1,051,106 + -0.0 1,051,107 - -0.0 1,051,107 - +0.1 1,051,107 - -3.6 1,051,111 + +1.0 1,051,111 + -0.2 1,051,111 + +0.0 1,051,112 - -0.6 1,051,112 - +0.3 1,051,252 + AMB_RS05080 0.26 +2.2 1,051,252 + AMB_RS05080 0.26 +0.5 1,051,252 + AMB_RS05080 0.26 -0.2 1,051,253 - AMB_RS05080 0.27 +0.1 1,051,253 - AMB_RS05080 0.27 -0.3 1,051,363 - AMB_RS05080 0.51 -0.1 1,051,363 - AMB_RS05080 0.51 +1.7 1,051,363 - AMB_RS05080 0.51 -0.4 1,051,363 - AMB_RS05080 0.51 +1.7 1,051,373 - AMB_RS05080 0.54 -0.1 1,051,373 - AMB_RS05080 0.54 -2.6 1,051,405 + AMB_RS05080 0.61 -0.5
Or see this region's nucleotide sequence