Experiment: without_iron2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01025 and AMB_RS01030 are separated by 28 nucleotides AMB_RS01030 and AMB_RS01035 are separated by 232 nucleotides AMB_RS01035 and AMB_RS01040 are separated by 171 nucleotides AMB_RS01040 and AMB_RS01045 are separated by 124 nucleotides
AMB_RS01025: AMB_RS01025 - molecular chaperone GroEL, at 227,557 to 229,215
_RS01025
AMB_RS01030: AMB_RS01030 - co-chaperone GroES, at 229,244 to 229,531
_RS01030
AMB_RS01035: AMB_RS01035 - DUF2076 domain-containing protein, at 229,764 to 230,453
_RS01035
AMB_RS01040: AMB_RS01040 - DUF1178 domain-containing protein, at 230,625 to 230,867
_RS01040
AMB_RS01045: AMB_RS01045 - NADH-quinone oxidoreductase subunit NuoB, at 230,992 to 231,528
_RS01045
Position (kb)
229
230
231 Strain fitness (log2 ratio)
-2
-1
0
1 at 229.232 kb on - strand at 229.684 kb on - strand at 229.724 kb on + strand at 229.724 kb on + strand at 229.724 kb on + strand at 229.724 kb on + strand at 229.724 kb on + strand at 229.724 kb on + strand at 229.725 kb on - strand at 229.725 kb on - strand at 229.725 kb on - strand at 229.725 kb on - strand at 229.893 kb on + strand, within AMB_RS01035 at 229.893 kb on + strand, within AMB_RS01035 at 229.894 kb on - strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.098 kb on - strand, within AMB_RS01035 at 230.098 kb on - strand, within AMB_RS01035 at 230.121 kb on + strand, within AMB_RS01035 at 230.122 kb on - strand, within AMB_RS01035 at 230.122 kb on - strand, within AMB_RS01035 at 230.122 kb on - strand, within AMB_RS01035 at 230.122 kb on - strand, within AMB_RS01035 at 230.122 kb on - strand, within AMB_RS01035 at 230.319 kb on + strand, within AMB_RS01035 at 230.319 kb on + strand, within AMB_RS01035 at 230.319 kb on + strand, within AMB_RS01035 at 230.319 kb on + strand, within AMB_RS01035 at 230.319 kb on + strand, within AMB_RS01035 at 230.319 kb on + strand, within AMB_RS01035 at 230.320 kb on - strand, within AMB_RS01035 at 230.320 kb on - strand, within AMB_RS01035 at 230.320 kb on - strand, within AMB_RS01035 at 230.320 kb on - strand, within AMB_RS01035 at 230.320 kb on - strand, within AMB_RS01035 at 230.320 kb on - strand, within AMB_RS01035 at 230.322 kb on + strand, within AMB_RS01035 at 230.322 kb on + strand, within AMB_RS01035 at 230.370 kb on + strand, within AMB_RS01035 at 230.451 kb on + strand at 230.451 kb on + strand at 230.452 kb on - strand at 230.471 kb on - strand at 230.471 kb on - strand at 230.487 kb on + strand at 230.488 kb on - strand at 230.497 kb on - strand at 230.556 kb on + strand at 230.556 kb on + strand at 230.557 kb on - strand at 230.588 kb on + strand at 230.588 kb on + strand at 230.588 kb on + strand at 230.589 kb on - strand at 230.589 kb on - strand at 230.590 kb on + strand at 230.634 kb on + strand at 230.634 kb on + strand at 230.635 kb on - strand at 230.635 kb on - strand at 230.635 kb on - strand at 230.635 kb on - strand at 230.993 kb on + strand at 230.993 kb on + strand at 230.994 kb on - strand at 231.116 kb on + strand, within AMB_RS01045 at 231.116 kb on + strand, within AMB_RS01045 at 231.116 kb on + strand, within AMB_RS01045 at 231.117 kb on - strand, within AMB_RS01045 at 231.117 kb on - strand, within AMB_RS01045 at 231.117 kb on - strand, within AMB_RS01045 at 231.117 kb on - strand, within AMB_RS01045 at 231.117 kb on - strand, within AMB_RS01045 at 231.117 kb on - strand, within AMB_RS01045 at 231.305 kb on + strand, within AMB_RS01045 at 231.305 kb on + strand, within AMB_RS01045 at 231.306 kb on - strand, within AMB_RS01045 at 231.306 kb on - strand, within AMB_RS01045
Per-strain Table
Position Strand Gene LocusTag Fraction without_iron2 remove 229,232 - -0.5 229,684 - +0.2 229,724 + +0.3 229,724 + +1.0 229,724 + +0.3 229,724 + -0.3 229,724 + -0.4 229,724 + -1.2 229,725 - -0.4 229,725 - -0.3 229,725 - -0.2 229,725 - -0.4 229,893 + AMB_RS01035 0.19 +0.2 229,893 + AMB_RS01035 0.19 +0.2 229,894 - AMB_RS01035 0.19 -0.5 230,097 + AMB_RS01035 0.48 +0.9 230,097 + AMB_RS01035 0.48 -0.2 230,097 + AMB_RS01035 0.48 -0.8 230,097 + AMB_RS01035 0.48 -0.4 230,097 + AMB_RS01035 0.48 -0.2 230,097 + AMB_RS01035 0.48 -0.3 230,097 + AMB_RS01035 0.48 -0.3 230,098 - AMB_RS01035 0.48 -0.0 230,098 - AMB_RS01035 0.48 +0.5 230,121 + AMB_RS01035 0.52 -0.3 230,122 - AMB_RS01035 0.52 +0.2 230,122 - AMB_RS01035 0.52 +0.3 230,122 - AMB_RS01035 0.52 +0.1 230,122 - AMB_RS01035 0.52 +0.3 230,122 - AMB_RS01035 0.52 -0.3 230,319 + AMB_RS01035 0.80 -0.7 230,319 + AMB_RS01035 0.80 +0.3 230,319 + AMB_RS01035 0.80 +1.5 230,319 + AMB_RS01035 0.80 -1.0 230,319 + AMB_RS01035 0.80 -1.9 230,319 + AMB_RS01035 0.80 -0.6 230,320 - AMB_RS01035 0.81 -0.2 230,320 - AMB_RS01035 0.81 -2.3 230,320 - AMB_RS01035 0.81 -0.3 230,320 - AMB_RS01035 0.81 +0.3 230,320 - AMB_RS01035 0.81 -0.0 230,320 - AMB_RS01035 0.81 -0.5 230,322 + AMB_RS01035 0.81 -0.0 230,322 + AMB_RS01035 0.81 +0.6 230,370 + AMB_RS01035 0.88 +0.0 230,451 + +0.2 230,451 + -0.1 230,452 - +0.4 230,471 - +0.0 230,471 - -0.5 230,487 + -0.3 230,488 - -0.9 230,497 - +0.5 230,556 + -0.2 230,556 + -0.7 230,557 - +0.8 230,588 + -0.4 230,588 + -0.7 230,588 + +0.0 230,589 - +0.4 230,589 - +1.1 230,590 + -0.6 230,634 + -0.7 230,634 + -0.5 230,635 - -0.3 230,635 - -0.5 230,635 - -0.1 230,635 - -0.5 230,993 + -0.8 230,993 + -0.1 230,994 - -0.7 231,116 + AMB_RS01045 0.23 -0.7 231,116 + AMB_RS01045 0.23 +0.1 231,116 + AMB_RS01045 0.23 +0.5 231,117 - AMB_RS01045 0.23 -0.2 231,117 - AMB_RS01045 0.23 +0.5 231,117 - AMB_RS01045 0.23 -0.1 231,117 - AMB_RS01045 0.23 -0.4 231,117 - AMB_RS01045 0.23 +0.3 231,117 - AMB_RS01045 0.23 +0.3 231,305 + AMB_RS01045 0.58 -0.5 231,305 + AMB_RS01045 0.58 +0.7 231,306 - AMB_RS01045 0.58 +0.8 231,306 - AMB_RS01045 0.58 +1.1
Or see this region's nucleotide sequence