Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4540 and EX28DRAFT_4541 are separated by 41 nucleotides EX28DRAFT_4541 and EX28DRAFT_4542 are separated by 186 nucleotides EX28DRAFT_4542 and EX28DRAFT_4543 are separated by 11 nucleotides EX28DRAFT_4543 and EX28DRAFT_4544 are separated by 49 nucleotides
EX28DRAFT_4540: EX28DRAFT_4540 - Endonuclease V (EC 3.1.21.-), at 23,343 to 24,014
_4540
EX28DRAFT_4541: EX28DRAFT_4541 - Uncharacterized protein conserved in bacteria, at 24,056 to 24,646
_4541
EX28DRAFT_4542: EX28DRAFT_4542 - Bacterial nucleoid DNA-binding protein, at 24,833 to 25,105
_4542
EX28DRAFT_4543: EX28DRAFT_4543 - Protein of unknown function (DUF1481), at 25,117 to 25,809
_4543
EX28DRAFT_4544: EX28DRAFT_4544 - phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 25,859 to 27,151
_4544
Position (kb) 24
25
26 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 24.021 kb on + strand at 24.152 kb on + strand, within EX28DRAFT_4541 at 24.194 kb on - strand, within EX28DRAFT_4541 at 24.197 kb on + strand, within EX28DRAFT_4541 at 24.424 kb on + strand, within EX28DRAFT_4541 at 24.424 kb on + strand, within EX28DRAFT_4541 at 24.424 kb on + strand, within EX28DRAFT_4541 at 24.424 kb on + strand, within EX28DRAFT_4541 at 24.600 kb on + strand at 24.717 kb on - strand at 24.755 kb on + strand at 24.756 kb on - strand at 24.756 kb on - strand at 24.800 kb on + strand at 24.801 kb on - strand at 24.827 kb on + strand at 24.832 kb on + strand at 24.858 kb on + strand at 24.858 kb on + strand at 24.859 kb on - strand at 24.895 kb on + strand, within EX28DRAFT_4542 at 24.913 kb on + strand, within EX28DRAFT_4542 at 25.006 kb on + strand, within EX28DRAFT_4542 at 25.079 kb on - strand at 25.099 kb on + strand at 25.103 kb on + strand at 25.104 kb on - strand at 25.129 kb on - strand at 25.344 kb on + strand, within EX28DRAFT_4543 at 25.344 kb on + strand, within EX28DRAFT_4543 at 25.344 kb on + strand, within EX28DRAFT_4543 at 25.345 kb on - strand, within EX28DRAFT_4543 at 25.345 kb on - strand, within EX28DRAFT_4543 at 25.345 kb on - strand, within EX28DRAFT_4543 at 25.406 kb on - strand, within EX28DRAFT_4543 at 25.406 kb on - strand, within EX28DRAFT_4543 at 25.465 kb on - strand, within EX28DRAFT_4543 at 25.679 kb on + strand, within EX28DRAFT_4543 at 25.679 kb on + strand, within EX28DRAFT_4543 at 25.679 kb on + strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.779 kb on + strand at 25.812 kb on + strand at 25.813 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 24,021 + +0.4 24,152 + EX28DRAFT_4541 0.16 +1.7 24,194 - EX28DRAFT_4541 0.23 +0.7 24,197 + EX28DRAFT_4541 0.24 -0.7 24,424 + EX28DRAFT_4541 0.62 +0.8 24,424 + EX28DRAFT_4541 0.62 -0.1 24,424 + EX28DRAFT_4541 0.62 -1.7 24,424 + EX28DRAFT_4541 0.62 +1.2 24,600 + -0.7 24,717 - -0.1 24,755 + -1.7 24,756 - -0.5 24,756 - -1.3 24,800 + +0.7 24,801 - +0.6 24,827 + +0.6 24,832 + -2.9 24,858 + +2.1 24,858 + +0.4 24,859 - +0.8 24,895 + EX28DRAFT_4542 0.23 +0.1 24,913 + EX28DRAFT_4542 0.29 -2.9 25,006 + EX28DRAFT_4542 0.63 -2.9 25,079 - +0.9 25,099 + -0.5 25,103 + +0.8 25,104 - -1.1 25,129 - +0.5 25,344 + EX28DRAFT_4543 0.33 +3.4 25,344 + EX28DRAFT_4543 0.33 -1.0 25,344 + EX28DRAFT_4543 0.33 +2.0 25,345 - EX28DRAFT_4543 0.33 +0.7 25,345 - EX28DRAFT_4543 0.33 -1.1 25,345 - EX28DRAFT_4543 0.33 +1.2 25,406 - EX28DRAFT_4543 0.42 -1.4 25,406 - EX28DRAFT_4543 0.42 -2.2 25,465 - EX28DRAFT_4543 0.50 +0.5 25,679 + EX28DRAFT_4543 0.81 -0.1 25,679 + EX28DRAFT_4543 0.81 +0.8 25,679 + EX28DRAFT_4543 0.81 -3.8 25,680 - EX28DRAFT_4543 0.81 +0.8 25,680 - EX28DRAFT_4543 0.81 -0.6 25,680 - EX28DRAFT_4543 0.81 +0.3 25,680 - EX28DRAFT_4543 0.81 +0.9 25,680 - EX28DRAFT_4543 0.81 -1.1 25,680 - EX28DRAFT_4543 0.81 +0.8 25,779 + +0.5 25,812 + -1.1 25,813 - -0.3
Or see this region's nucleotide sequence