Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4504 and EX28DRAFT_4505 overlap by 4 nucleotides EX28DRAFT_4505 and EX28DRAFT_4506 are separated by 170 nucleotides EX28DRAFT_4506 and EX28DRAFT_4507 are separated by 42 nucleotides EX28DRAFT_4507 and EX28DRAFT_4508 are separated by 39 nucleotides EX28DRAFT_4508 and EX28DRAFT_4509 are separated by 0 nucleotides
EX28DRAFT_4504: EX28DRAFT_4504 - Mg-dependent DNase, at 70,311 to 71,093
_4504
EX28DRAFT_4505: EX28DRAFT_4505 - transcription elongation factor/antiterminator RfaH, at 71,090 to 71,581
_4505
EX28DRAFT_4506: EX28DRAFT_4506 - UbiD family decarboxylase, at 71,752 to 73,245
_4506
EX28DRAFT_4507: EX28DRAFT_4507 - 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases, at 73,288 to 73,989
_4507
EX28DRAFT_4508: EX28DRAFT_4508 - hypothetical protein, at 74,029 to 74,172
_4508
EX28DRAFT_4509: EX28DRAFT_4509 - fatty oxidation complex, beta subunit FadA, at 74,173 to 75,336
_4509
Position (kb)
71
72
73
74 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 70.818 kb on + strand, within EX28DRAFT_4504 at 70.927 kb on - strand, within EX28DRAFT_4504 at 70.944 kb on - strand, within EX28DRAFT_4504 at 70.983 kb on + strand, within EX28DRAFT_4504 at 71.092 kb on - strand at 71.116 kb on + strand at 71.130 kb on - strand at 71.161 kb on + strand, within EX28DRAFT_4505 at 71.162 kb on - strand, within EX28DRAFT_4505 at 71.162 kb on - strand, within EX28DRAFT_4505 at 71.225 kb on - strand, within EX28DRAFT_4505 at 71.286 kb on + strand, within EX28DRAFT_4505 at 71.286 kb on + strand, within EX28DRAFT_4505 at 71.422 kb on - strand, within EX28DRAFT_4505 at 71.565 kb on - strand at 71.569 kb on - strand at 71.581 kb on + strand at 71.581 kb on + strand at 71.582 kb on - strand at 71.582 kb on - strand at 71.647 kb on + strand at 71.648 kb on - strand at 71.650 kb on - strand at 71.727 kb on - strand at 73.237 kb on - strand at 73.255 kb on + strand at 73.264 kb on + strand at 73.264 kb on + strand at 73.264 kb on + strand at 73.264 kb on + strand at 73.265 kb on - strand at 73.340 kb on + strand at 73.340 kb on + strand at 73.340 kb on + strand at 73.340 kb on + strand at 73.340 kb on + strand at 73.340 kb on + strand at 73.758 kb on - strand, within EX28DRAFT_4507 at 73.883 kb on - strand, within EX28DRAFT_4507 at 73.883 kb on - strand, within EX28DRAFT_4507 at 73.883 kb on - strand, within EX28DRAFT_4507 at 73.988 kb on - strand at 74.030 kb on + strand at 74.030 kb on + strand at 74.103 kb on + strand, within EX28DRAFT_4508 at 74.104 kb on - strand, within EX28DRAFT_4508 at 74.104 kb on - strand, within EX28DRAFT_4508 at 74.174 kb on + strand at 74.175 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 70,818 + EX28DRAFT_4504 0.65 +0.9 70,927 - EX28DRAFT_4504 0.79 +1.0 70,944 - EX28DRAFT_4504 0.81 +0.8 70,983 + EX28DRAFT_4504 0.86 +0.5 71,092 - +0.5 71,116 + +3.3 71,130 - -2.1 71,161 + EX28DRAFT_4505 0.14 +0.5 71,162 - EX28DRAFT_4505 0.15 +1.8 71,162 - EX28DRAFT_4505 0.15 -0.1 71,225 - EX28DRAFT_4505 0.27 +0.7 71,286 + EX28DRAFT_4505 0.40 +0.9 71,286 + EX28DRAFT_4505 0.40 +1.3 71,422 - EX28DRAFT_4505 0.67 +1.3 71,565 - -1.7 71,569 - -0.1 71,581 + -1.7 71,581 + -0.3 71,582 - -1.9 71,582 - -0.0 71,647 + -0.2 71,648 - -2.5 71,650 - -0.7 71,727 - +1.1 73,237 - +0.1 73,255 + +0.4 73,264 + +1.7 73,264 + +1.1 73,264 + +1.3 73,264 + -2.7 73,265 - -0.3 73,340 + -3.3 73,340 + +1.5 73,340 + +0.1 73,340 + -0.5 73,340 + -0.5 73,340 + +0.1 73,758 - EX28DRAFT_4507 0.67 -1.5 73,883 - EX28DRAFT_4507 0.85 -0.1 73,883 - EX28DRAFT_4507 0.85 +0.5 73,883 - EX28DRAFT_4507 0.85 -2.4 73,988 - +1.2 74,030 + +0.5 74,030 + +0.5 74,103 + EX28DRAFT_4508 0.51 -2.5 74,104 - EX28DRAFT_4508 0.52 -1.4 74,104 - EX28DRAFT_4508 0.52 +0.7 74,174 + -0.8 74,175 - -1.2
Or see this region's nucleotide sequence