Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3657 and EX28DRAFT_3658 are separated by 44 nucleotides EX28DRAFT_3658 and EX28DRAFT_3659 overlap by 17 nucleotides EX28DRAFT_3659 and EX28DRAFT_3660 overlap by 25 nucleotides
EX28DRAFT_3657: EX28DRAFT_3657 - Uncharacterized membrane-associated protein, at 324,557 to 325,324
_3657
EX28DRAFT_3658: EX28DRAFT_3658 - thiamine ABC transporter, ATP-binding protein, at 325,369 to 326,070
_3658
EX28DRAFT_3659: EX28DRAFT_3659 - thiamine ABC transporter, permease protein, at 326,054 to 327,664
_3659
EX28DRAFT_3660: EX28DRAFT_3660 - thiamine ABC transporter, periplasmic binding protein, at 327,640 to 328,623
_3660
Position (kb) 326
327
328 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 325.088 kb on - strand, within EX28DRAFT_3657 at 325.190 kb on + strand, within EX28DRAFT_3657 at 325.191 kb on - strand, within EX28DRAFT_3657 at 325.503 kb on + strand, within EX28DRAFT_3658 at 325.864 kb on - strand, within EX28DRAFT_3658 at 325.944 kb on + strand, within EX28DRAFT_3658 at 325.945 kb on - strand, within EX28DRAFT_3658 at 325.945 kb on - strand, within EX28DRAFT_3658 at 326.098 kb on + strand at 326.102 kb on + strand at 326.187 kb on + strand at 326.489 kb on + strand, within EX28DRAFT_3659 at 326.870 kb on - strand, within EX28DRAFT_3659 at 326.953 kb on - strand, within EX28DRAFT_3659 at 326.974 kb on - strand, within EX28DRAFT_3659 at 327.052 kb on + strand, within EX28DRAFT_3659 at 327.215 kb on - strand, within EX28DRAFT_3659 at 327.341 kb on + strand, within EX28DRAFT_3659 at 327.627 kb on - strand at 327.670 kb on + strand at 327.671 kb on - strand at 327.769 kb on - strand, within EX28DRAFT_3660 at 327.769 kb on - strand, within EX28DRAFT_3660 at 327.779 kb on + strand, within EX28DRAFT_3660 at 327.903 kb on - strand, within EX28DRAFT_3660 at 327.932 kb on + strand, within EX28DRAFT_3660 at 327.932 kb on + strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.936 kb on + strand, within EX28DRAFT_3660 at 327.936 kb on + strand, within EX28DRAFT_3660 at 327.937 kb on - strand, within EX28DRAFT_3660 at 327.937 kb on - strand, within EX28DRAFT_3660 at 327.937 kb on - strand, within EX28DRAFT_3660 at 327.978 kb on + strand, within EX28DRAFT_3660 at 327.978 kb on + strand, within EX28DRAFT_3660 at 327.979 kb on - strand, within EX28DRAFT_3660 at 327.981 kb on - strand, within EX28DRAFT_3660 at 327.981 kb on - strand, within EX28DRAFT_3660 at 327.981 kb on - strand, within EX28DRAFT_3660 at 328.238 kb on + strand, within EX28DRAFT_3660 at 328.253 kb on + strand, within EX28DRAFT_3660 at 328.545 kb on - strand at 328.545 kb on - strand at 328.550 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 325,088 - EX28DRAFT_3657 0.69 +1.5 325,190 + EX28DRAFT_3657 0.82 +0.6 325,191 - EX28DRAFT_3657 0.83 -0.6 325,503 + EX28DRAFT_3658 0.19 -0.2 325,864 - EX28DRAFT_3658 0.71 +0.1 325,944 + EX28DRAFT_3658 0.82 +0.7 325,945 - EX28DRAFT_3658 0.82 +0.5 325,945 - EX28DRAFT_3658 0.82 -2.1 326,098 + +1.4 326,102 + +0.2 326,187 + -0.7 326,489 + EX28DRAFT_3659 0.27 -0.2 326,870 - EX28DRAFT_3659 0.51 -4.2 326,953 - EX28DRAFT_3659 0.56 -0.2 326,974 - EX28DRAFT_3659 0.57 -0.5 327,052 + EX28DRAFT_3659 0.62 +0.5 327,215 - EX28DRAFT_3659 0.72 -2.2 327,341 + EX28DRAFT_3659 0.80 -4.5 327,627 - -0.6 327,670 + -0.2 327,671 - -0.6 327,769 - EX28DRAFT_3660 0.13 +0.8 327,769 - EX28DRAFT_3660 0.13 -0.2 327,779 + EX28DRAFT_3660 0.14 +0.8 327,903 - EX28DRAFT_3660 0.27 -0.8 327,932 + EX28DRAFT_3660 0.30 -0.3 327,932 + EX28DRAFT_3660 0.30 -0.5 327,933 - EX28DRAFT_3660 0.30 +0.0 327,933 - EX28DRAFT_3660 0.30 +1.5 327,933 - EX28DRAFT_3660 0.30 +0.9 327,933 - EX28DRAFT_3660 0.30 -0.0 327,933 - EX28DRAFT_3660 0.30 -0.3 327,933 - EX28DRAFT_3660 0.30 +0.7 327,933 - EX28DRAFT_3660 0.30 -1.1 327,933 - EX28DRAFT_3660 0.30 +0.5 327,933 - EX28DRAFT_3660 0.30 +0.7 327,936 + EX28DRAFT_3660 0.30 -1.2 327,936 + EX28DRAFT_3660 0.30 +0.2 327,937 - EX28DRAFT_3660 0.30 -0.3 327,937 - EX28DRAFT_3660 0.30 +0.5 327,937 - EX28DRAFT_3660 0.30 +0.2 327,978 + EX28DRAFT_3660 0.34 +1.0 327,978 + EX28DRAFT_3660 0.34 -0.4 327,979 - EX28DRAFT_3660 0.34 +0.2 327,981 - EX28DRAFT_3660 0.35 -0.4 327,981 - EX28DRAFT_3660 0.35 +0.2 327,981 - EX28DRAFT_3660 0.35 -0.5 328,238 + EX28DRAFT_3660 0.61 -0.1 328,253 + EX28DRAFT_3660 0.62 -0.7 328,545 - +0.1 328,545 - +0.4 328,550 + -0.4
Or see this region's nucleotide sequence