Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3590 and EX28DRAFT_3591 are separated by 109 nucleotides EX28DRAFT_3591 and EX28DRAFT_3592 are separated by 52 nucleotides
EX28DRAFT_3590: EX28DRAFT_3590 - deoxyribose-phosphate aldolase, at 247,302 to 248,081
_3590
EX28DRAFT_3591: EX28DRAFT_3591 - thymidine phosphorylase (EC 2.4.2.4), at 248,191 to 249,513
_3591
EX28DRAFT_3592: EX28DRAFT_3592 - phosphopentomutase, at 249,566 to 250,789
_3592
Position (kb) 248
249
250 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 247.249 kb on + strand at 247.275 kb on + strand at 247.301 kb on + strand at 247.313 kb on - strand at 247.373 kb on + strand at 247.374 kb on - strand at 247.443 kb on - strand, within EX28DRAFT_3590 at 247.443 kb on - strand, within EX28DRAFT_3590 at 247.519 kb on + strand, within EX28DRAFT_3590 at 247.523 kb on + strand, within EX28DRAFT_3590 at 247.523 kb on + strand, within EX28DRAFT_3590 at 247.524 kb on - strand, within EX28DRAFT_3590 at 247.677 kb on - strand, within EX28DRAFT_3590 at 247.758 kb on - strand, within EX28DRAFT_3590 at 247.999 kb on - strand, within EX28DRAFT_3590 at 248.060 kb on + strand at 248.077 kb on - strand at 248.211 kb on + strand at 248.241 kb on - strand at 248.340 kb on + strand, within EX28DRAFT_3591 at 248.340 kb on + strand, within EX28DRAFT_3591 at 248.340 kb on + strand, within EX28DRAFT_3591 at 248.341 kb on - strand, within EX28DRAFT_3591 at 248.625 kb on + strand, within EX28DRAFT_3591 at 248.692 kb on + strand, within EX28DRAFT_3591 at 248.831 kb on - strand, within EX28DRAFT_3591 at 248.970 kb on - strand, within EX28DRAFT_3591 at 249.041 kb on + strand, within EX28DRAFT_3591 at 249.041 kb on + strand, within EX28DRAFT_3591 at 249.042 kb on - strand, within EX28DRAFT_3591 at 249.042 kb on - strand, within EX28DRAFT_3591 at 249.187 kb on + strand, within EX28DRAFT_3591 at 249.187 kb on + strand, within EX28DRAFT_3591 at 249.325 kb on + strand, within EX28DRAFT_3591 at 249.326 kb on - strand, within EX28DRAFT_3591 at 249.511 kb on + strand at 249.517 kb on + strand at 249.519 kb on + strand at 249.520 kb on - strand at 249.565 kb on + strand at 249.580 kb on + strand at 249.580 kb on + strand at 249.581 kb on - strand at 249.911 kb on + strand, within EX28DRAFT_3592 at 249.912 kb on - strand, within EX28DRAFT_3592 at 250.355 kb on + strand, within EX28DRAFT_3592 at 250.355 kb on + strand, within EX28DRAFT_3592 at 250.355 kb on + strand, within EX28DRAFT_3592 at 250.355 kb on + strand, within EX28DRAFT_3592 at 250.355 kb on + strand, within EX28DRAFT_3592 at 250.356 kb on - strand, within EX28DRAFT_3592 at 250.356 kb on - strand, within EX28DRAFT_3592
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 247,249 + -0.9 247,275 + +1.5 247,301 + -1.3 247,313 - -0.5 247,373 + +0.1 247,374 - +0.4 247,443 - EX28DRAFT_3590 0.18 +0.7 247,443 - EX28DRAFT_3590 0.18 -1.1 247,519 + EX28DRAFT_3590 0.28 +1.1 247,523 + EX28DRAFT_3590 0.28 -0.0 247,523 + EX28DRAFT_3590 0.28 -0.4 247,524 - EX28DRAFT_3590 0.28 -1.8 247,677 - EX28DRAFT_3590 0.48 -3.1 247,758 - EX28DRAFT_3590 0.58 -0.8 247,999 - EX28DRAFT_3590 0.89 -0.2 248,060 + -0.5 248,077 - +0.4 248,211 + +0.2 248,241 - -0.2 248,340 + EX28DRAFT_3591 0.11 +0.2 248,340 + EX28DRAFT_3591 0.11 +0.4 248,340 + EX28DRAFT_3591 0.11 -2.8 248,341 - EX28DRAFT_3591 0.11 -1.5 248,625 + EX28DRAFT_3591 0.33 +0.7 248,692 + EX28DRAFT_3591 0.38 +0.2 248,831 - EX28DRAFT_3591 0.48 -0.1 248,970 - EX28DRAFT_3591 0.59 -0.6 249,041 + EX28DRAFT_3591 0.64 -0.3 249,041 + EX28DRAFT_3591 0.64 +0.5 249,042 - EX28DRAFT_3591 0.64 +0.9 249,042 - EX28DRAFT_3591 0.64 -1.2 249,187 + EX28DRAFT_3591 0.75 -0.6 249,187 + EX28DRAFT_3591 0.75 +0.5 249,325 + EX28DRAFT_3591 0.86 -0.9 249,326 - EX28DRAFT_3591 0.86 +0.7 249,511 + +0.3 249,517 + +0.7 249,519 + -3.9 249,520 - +1.6 249,565 + +0.5 249,580 + +0.4 249,580 + -1.0 249,581 - +2.9 249,911 + EX28DRAFT_3592 0.28 -1.4 249,912 - EX28DRAFT_3592 0.28 +0.2 250,355 + EX28DRAFT_3592 0.64 +0.1 250,355 + EX28DRAFT_3592 0.64 -0.3 250,355 + EX28DRAFT_3592 0.64 -2.9 250,355 + EX28DRAFT_3592 0.64 +0.6 250,355 + EX28DRAFT_3592 0.64 +0.7 250,356 - EX28DRAFT_3592 0.65 -1.6 250,356 - EX28DRAFT_3592 0.65 -4.3
Or see this region's nucleotide sequence