Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3201 and EX28DRAFT_3202 are separated by 128 nucleotides EX28DRAFT_3202 and EX28DRAFT_3203 overlap by 21 nucleotides EX28DRAFT_3203 and EX28DRAFT_3204 are separated by 14 nucleotides EX28DRAFT_3204 and EX28DRAFT_3205 overlap by 14 nucleotides EX28DRAFT_3205 and EX28DRAFT_3206 overlap by 7 nucleotides
EX28DRAFT_3201: EX28DRAFT_3201 - Predicted nucleoside-diphosphate-sugar epimerases, at 502,185 to 502,829
_3201
EX28DRAFT_3202: EX28DRAFT_3202 - intracellular protease, PfpI family, at 502,958 to 503,476
_3202
EX28DRAFT_3203: EX28DRAFT_3203 - Uncharacterized protein conserved in bacteria, at 503,456 to 503,890
_3203
EX28DRAFT_3204: EX28DRAFT_3204 - Predicted endonuclease containing a URI domain, at 503,905 to 504,234
_3204
EX28DRAFT_3205: EX28DRAFT_3205 - Predicted acetyltransferase, at 504,221 to 504,724
_3205
EX28DRAFT_3206: EX28DRAFT_3206 - Putative lipid carrier protein, at 504,718 to 505,242
_3206
Position (kb) 503
504 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 502.566 kb on - strand, within EX28DRAFT_3201 at 502.568 kb on - strand, within EX28DRAFT_3201 at 502.581 kb on - strand, within EX28DRAFT_3201 at 502.744 kb on + strand, within EX28DRAFT_3201 at 502.744 kb on + strand, within EX28DRAFT_3201 at 502.745 kb on - strand, within EX28DRAFT_3201 at 502.745 kb on - strand, within EX28DRAFT_3201 at 502.745 kb on - strand, within EX28DRAFT_3201 at 502.745 kb on - strand, within EX28DRAFT_3201 at 502.745 kb on - strand, within EX28DRAFT_3201 at 502.745 kb on - strand, within EX28DRAFT_3201 at 502.745 kb on - strand, within EX28DRAFT_3201 at 502.812 kb on - strand at 502.885 kb on - strand at 502.885 kb on - strand at 502.885 kb on - strand at 502.885 kb on - strand at 502.885 kb on - strand at 502.885 kb on - strand at 502.895 kb on + strand at 502.895 kb on + strand at 502.896 kb on - strand at 502.901 kb on + strand at 502.901 kb on + strand at 502.905 kb on + strand at 502.905 kb on + strand at 502.905 kb on + strand at 502.905 kb on + strand at 502.906 kb on - strand at 502.906 kb on - strand at 502.906 kb on - strand at 502.909 kb on + strand at 503.474 kb on + strand at 503.590 kb on - strand, within EX28DRAFT_3203 at 503.590 kb on - strand, within EX28DRAFT_3203 at 503.645 kb on - strand, within EX28DRAFT_3203 at 503.761 kb on - strand, within EX28DRAFT_3203 at 503.784 kb on + strand, within EX28DRAFT_3203 at 503.784 kb on + strand, within EX28DRAFT_3203 at 503.785 kb on - strand, within EX28DRAFT_3203 at 503.787 kb on + strand, within EX28DRAFT_3203 at 503.787 kb on + strand, within EX28DRAFT_3203 at 503.790 kb on + strand, within EX28DRAFT_3203 at 503.791 kb on - strand, within EX28DRAFT_3203 at 503.909 kb on + strand at 503.909 kb on + strand at 503.910 kb on - strand at 503.910 kb on - strand at 503.926 kb on - strand at 503.926 kb on - strand at 503.941 kb on + strand, within EX28DRAFT_3204 at 503.942 kb on - strand, within EX28DRAFT_3204 at 503.962 kb on + strand, within EX28DRAFT_3204 at 503.992 kb on + strand, within EX28DRAFT_3204 at 504.081 kb on - strand, within EX28DRAFT_3204 at 504.081 kb on - strand, within EX28DRAFT_3204 at 504.222 kb on + strand at 504.223 kb on - strand at 504.223 kb on - strand at 504.223 kb on - strand at 504.444 kb on + strand, within EX28DRAFT_3205 at 504.477 kb on + strand, within EX28DRAFT_3205 at 504.558 kb on + strand, within EX28DRAFT_3205 at 504.605 kb on - strand, within EX28DRAFT_3205 at 504.734 kb on - strand at 504.832 kb on + strand, within EX28DRAFT_3206 at 504.833 kb on - strand, within EX28DRAFT_3206 at 504.863 kb on + strand, within EX28DRAFT_3206
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 502,566 - EX28DRAFT_3201 0.59 +1.8 502,568 - EX28DRAFT_3201 0.59 -2.4 502,581 - EX28DRAFT_3201 0.61 +1.1 502,744 + EX28DRAFT_3201 0.87 +0.3 502,744 + EX28DRAFT_3201 0.87 -2.8 502,745 - EX28DRAFT_3201 0.87 -0.4 502,745 - EX28DRAFT_3201 0.87 -0.9 502,745 - EX28DRAFT_3201 0.87 +0.8 502,745 - EX28DRAFT_3201 0.87 +1.9 502,745 - EX28DRAFT_3201 0.87 +0.3 502,745 - EX28DRAFT_3201 0.87 -1.6 502,745 - EX28DRAFT_3201 0.87 +1.4 502,812 - -1.4 502,885 - +0.2 502,885 - -1.1 502,885 - -0.8 502,885 - +0.6 502,885 - -0.5 502,885 - +0.3 502,895 + +0.3 502,895 + +0.7 502,896 - -2.4 502,901 + +0.6 502,901 + -1.4 502,905 + -2.2 502,905 + +0.2 502,905 + -1.1 502,905 + +1.0 502,906 - -1.1 502,906 - -0.1 502,906 - -0.4 502,909 + -3.7 503,474 + +1.9 503,590 - EX28DRAFT_3203 0.31 -2.2 503,590 - EX28DRAFT_3203 0.31 +1.7 503,645 - EX28DRAFT_3203 0.43 +0.2 503,761 - EX28DRAFT_3203 0.70 +1.2 503,784 + EX28DRAFT_3203 0.75 -0.8 503,784 + EX28DRAFT_3203 0.75 +0.6 503,785 - EX28DRAFT_3203 0.76 -0.5 503,787 + EX28DRAFT_3203 0.76 +0.3 503,787 + EX28DRAFT_3203 0.76 +0.2 503,790 + EX28DRAFT_3203 0.77 +0.7 503,791 - EX28DRAFT_3203 0.77 +0.3 503,909 + -1.3 503,909 + +0.2 503,910 - -0.4 503,910 - -0.3 503,926 - -0.1 503,926 - +0.3 503,941 + EX28DRAFT_3204 0.11 -0.3 503,942 - EX28DRAFT_3204 0.11 +0.9 503,962 + EX28DRAFT_3204 0.17 -0.2 503,992 + EX28DRAFT_3204 0.26 -0.2 504,081 - EX28DRAFT_3204 0.53 -3.2 504,081 - EX28DRAFT_3204 0.53 +1.4 504,222 + -0.6 504,223 - +2.3 504,223 - -0.3 504,223 - +0.3 504,444 + EX28DRAFT_3205 0.44 +1.6 504,477 + EX28DRAFT_3205 0.51 +0.3 504,558 + EX28DRAFT_3205 0.67 +0.7 504,605 - EX28DRAFT_3205 0.76 +1.1 504,734 - -0.8 504,832 + EX28DRAFT_3206 0.22 +0.3 504,833 - EX28DRAFT_3206 0.22 +0.1 504,863 + EX28DRAFT_3206 0.28 +0.3
Or see this region's nucleotide sequence