Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3004 and EX28DRAFT_3005 are separated by 10 nucleotides EX28DRAFT_3005 and EX28DRAFT_3006 overlap by 4 nucleotides EX28DRAFT_3006 and EX28DRAFT_3007 are separated by 16 nucleotides
EX28DRAFT_3004: EX28DRAFT_3004 - citrate lyase, alpha subunit, at 311,948 to 313,471
_3004
EX28DRAFT_3005: EX28DRAFT_3005 - citrate lyase, beta subunit, at 313,482 to 314,357
_3005
EX28DRAFT_3006: EX28DRAFT_3006 - citrate lyase acyl carrier protein, at 314,354 to 314,647
_3006
EX28DRAFT_3007: EX28DRAFT_3007 - [citrate (pro-3S)-lyase] ligase, at 314,664 to 315,686
_3007
Position (kb) 314
315 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 313.422 kb on - strand at 313.478 kb on - strand at 313.709 kb on + strand, within EX28DRAFT_3005 at 313.709 kb on + strand, within EX28DRAFT_3005 at 313.838 kb on - strand, within EX28DRAFT_3005 at 313.838 kb on - strand, within EX28DRAFT_3005 at 314.050 kb on + strand, within EX28DRAFT_3005 at 314.051 kb on - strand, within EX28DRAFT_3005 at 314.182 kb on + strand, within EX28DRAFT_3005 at 314.183 kb on - strand, within EX28DRAFT_3005 at 314.183 kb on - strand, within EX28DRAFT_3005 at 314.285 kb on - strand at 314.288 kb on + strand at 314.288 kb on + strand at 314.337 kb on - strand at 314.536 kb on + strand, within EX28DRAFT_3006 at 314.665 kb on + strand at 314.789 kb on + strand, within EX28DRAFT_3007 at 314.918 kb on + strand, within EX28DRAFT_3007 at 314.939 kb on + strand, within EX28DRAFT_3007 at 315.011 kb on - strand, within EX28DRAFT_3007 at 315.040 kb on + strand, within EX28DRAFT_3007 at 315.040 kb on + strand, within EX28DRAFT_3007 at 315.040 kb on + strand, within EX28DRAFT_3007
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 313,422 - -0.3 313,478 - -0.3 313,709 + EX28DRAFT_3005 0.26 +0.4 313,709 + EX28DRAFT_3005 0.26 -0.3 313,838 - EX28DRAFT_3005 0.41 -1.9 313,838 - EX28DRAFT_3005 0.41 +0.4 314,050 + EX28DRAFT_3005 0.65 +0.4 314,051 - EX28DRAFT_3005 0.65 -0.5 314,182 + EX28DRAFT_3005 0.80 +0.1 314,183 - EX28DRAFT_3005 0.80 -0.1 314,183 - EX28DRAFT_3005 0.80 +0.7 314,285 - -2.3 314,288 + +0.2 314,288 + -0.1 314,337 - -1.0 314,536 + EX28DRAFT_3006 0.62 -2.0 314,665 + -0.4 314,789 + EX28DRAFT_3007 0.12 -0.4 314,918 + EX28DRAFT_3007 0.25 -3.7 314,939 + EX28DRAFT_3007 0.27 +0.8 315,011 - EX28DRAFT_3007 0.34 +0.6 315,040 + EX28DRAFT_3007 0.37 +0.1 315,040 + EX28DRAFT_3007 0.37 +0.6 315,040 + EX28DRAFT_3007 0.37 -0.7
Or see this region's nucleotide sequence