Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2993 and EX28DRAFT_2994 are separated by 63 nucleotides EX28DRAFT_2994 and EX28DRAFT_2995 are separated by 82 nucleotides EX28DRAFT_2995 and EX28DRAFT_2996 are separated by 56 nucleotides EX28DRAFT_2996 and EX28DRAFT_2997 overlap by 1 nucleotides
EX28DRAFT_2993: EX28DRAFT_2993 - putative oxygen-independent coproporphyrinogen III oxidase, at 302,984 to 304,126
_2993
EX28DRAFT_2994: EX28DRAFT_2994 - Uncharacterized protein conserved in bacteria, at 304,190 to 304,528
_2994
EX28DRAFT_2995: EX28DRAFT_2995 - Protein of unknown function (DUF2884), at 304,611 to 305,327
_2995
EX28DRAFT_2996: EX28DRAFT_2996 - Uncharacterized protein conserved in bacteria, at 305,384 to 305,710
_2996
EX28DRAFT_2997: EX28DRAFT_2997 - tRNA (guanine-N(7)-)-methyltransferase, at 305,710 to 306,429
_2997
Position (kb) 304
305
306 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 303.667 kb on - strand, within EX28DRAFT_2993 at 303.667 kb on - strand, within EX28DRAFT_2993 at 303.720 kb on - strand, within EX28DRAFT_2993 at 303.744 kb on - strand, within EX28DRAFT_2993 at 304.053 kb on - strand at 304.086 kb on + strand at 304.237 kb on - strand, within EX28DRAFT_2994 at 304.277 kb on + strand, within EX28DRAFT_2994 at 304.279 kb on + strand, within EX28DRAFT_2994 at 304.279 kb on + strand, within EX28DRAFT_2994 at 304.279 kb on + strand, within EX28DRAFT_2994 at 304.279 kb on + strand, within EX28DRAFT_2994 at 304.310 kb on + strand, within EX28DRAFT_2994 at 304.311 kb on - strand, within EX28DRAFT_2994 at 304.311 kb on - strand, within EX28DRAFT_2994 at 304.311 kb on - strand, within EX28DRAFT_2994 at 304.311 kb on - strand, within EX28DRAFT_2994 at 304.311 kb on - strand, within EX28DRAFT_2994 at 304.311 kb on - strand, within EX28DRAFT_2994 at 304.388 kb on + strand, within EX28DRAFT_2994 at 304.437 kb on + strand, within EX28DRAFT_2994 at 304.437 kb on + strand, within EX28DRAFT_2994 at 304.450 kb on + strand, within EX28DRAFT_2994 at 304.479 kb on + strand, within EX28DRAFT_2994 at 304.489 kb on + strand, within EX28DRAFT_2994 at 304.489 kb on + strand, within EX28DRAFT_2994 at 304.526 kb on + strand at 304.556 kb on + strand at 304.556 kb on + strand at 304.613 kb on - strand at 304.617 kb on - strand at 304.617 kb on - strand at 304.894 kb on + strand, within EX28DRAFT_2995 at 304.895 kb on - strand, within EX28DRAFT_2995 at 305.229 kb on + strand, within EX28DRAFT_2995 at 305.331 kb on - strand at 305.331 kb on - strand at 305.360 kb on + strand at 305.360 kb on + strand at 305.383 kb on - strand at 305.383 kb on - strand at 305.385 kb on + strand at 305.386 kb on - strand at 305.386 kb on - strand at 305.452 kb on + strand, within EX28DRAFT_2996 at 305.452 kb on + strand, within EX28DRAFT_2996 at 305.606 kb on + strand, within EX28DRAFT_2996 at 305.606 kb on + strand, within EX28DRAFT_2996 at 305.607 kb on - strand, within EX28DRAFT_2996 at 305.712 kb on - strand at 305.731 kb on + strand at 305.731 kb on + strand at 305.810 kb on + strand, within EX28DRAFT_2997 at 305.811 kb on - strand, within EX28DRAFT_2997 at 305.811 kb on - strand, within EX28DRAFT_2997 at 305.811 kb on - strand, within EX28DRAFT_2997 at 305.868 kb on + strand, within EX28DRAFT_2997 at 305.868 kb on + strand, within EX28DRAFT_2997 at 305.868 kb on + strand, within EX28DRAFT_2997 at 305.868 kb on + strand, within EX28DRAFT_2997 at 305.869 kb on - strand, within EX28DRAFT_2997 at 305.869 kb on - strand, within EX28DRAFT_2997 at 305.879 kb on + strand, within EX28DRAFT_2997 at 305.902 kb on - strand, within EX28DRAFT_2997 at 305.929 kb on - strand, within EX28DRAFT_2997 at 305.973 kb on + strand, within EX28DRAFT_2997 at 305.977 kb on - strand, within EX28DRAFT_2997 at 305.977 kb on - strand, within EX28DRAFT_2997 at 306.048 kb on + strand, within EX28DRAFT_2997
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 303,667 - EX28DRAFT_2993 0.60 -0.8 303,667 - EX28DRAFT_2993 0.60 -0.3 303,720 - EX28DRAFT_2993 0.64 +0.4 303,744 - EX28DRAFT_2993 0.66 +0.0 304,053 - +0.3 304,086 + +2.0 304,237 - EX28DRAFT_2994 0.14 +1.6 304,277 + EX28DRAFT_2994 0.26 +0.0 304,279 + EX28DRAFT_2994 0.26 -0.4 304,279 + EX28DRAFT_2994 0.26 -0.4 304,279 + EX28DRAFT_2994 0.26 +1.0 304,279 + EX28DRAFT_2994 0.26 +0.7 304,310 + EX28DRAFT_2994 0.35 -1.7 304,311 - EX28DRAFT_2994 0.36 +0.3 304,311 - EX28DRAFT_2994 0.36 -3.0 304,311 - EX28DRAFT_2994 0.36 -0.3 304,311 - EX28DRAFT_2994 0.36 -0.6 304,311 - EX28DRAFT_2994 0.36 +0.7 304,311 - EX28DRAFT_2994 0.36 -0.5 304,388 + EX28DRAFT_2994 0.58 -4.0 304,437 + EX28DRAFT_2994 0.73 -0.3 304,437 + EX28DRAFT_2994 0.73 -2.4 304,450 + EX28DRAFT_2994 0.77 +0.6 304,479 + EX28DRAFT_2994 0.85 -1.2 304,489 + EX28DRAFT_2994 0.88 -0.8 304,489 + EX28DRAFT_2994 0.88 +1.9 304,526 + +0.8 304,556 + -1.4 304,556 + +0.9 304,613 - -4.2 304,617 - -1.1 304,617 - -2.2 304,894 + EX28DRAFT_2995 0.39 +0.2 304,895 - EX28DRAFT_2995 0.40 +0.2 305,229 + EX28DRAFT_2995 0.86 +1.9 305,331 - -3.1 305,331 - -3.8 305,360 + -2.2 305,360 + +1.1 305,383 - +0.3 305,383 - +0.1 305,385 + +0.2 305,386 - +0.2 305,386 - +0.5 305,452 + EX28DRAFT_2996 0.21 +0.4 305,452 + EX28DRAFT_2996 0.21 -1.3 305,606 + EX28DRAFT_2996 0.68 -1.3 305,606 + EX28DRAFT_2996 0.68 +1.8 305,607 - EX28DRAFT_2996 0.68 -0.3 305,712 - -2.2 305,731 + +1.5 305,731 + +1.1 305,810 + EX28DRAFT_2997 0.14 -0.8 305,811 - EX28DRAFT_2997 0.14 -0.6 305,811 - EX28DRAFT_2997 0.14 -1.2 305,811 - EX28DRAFT_2997 0.14 -1.4 305,868 + EX28DRAFT_2997 0.22 -1.8 305,868 + EX28DRAFT_2997 0.22 -1.7 305,868 + EX28DRAFT_2997 0.22 -0.8 305,868 + EX28DRAFT_2997 0.22 -1.4 305,869 - EX28DRAFT_2997 0.22 +0.3 305,869 - EX28DRAFT_2997 0.22 -0.7 305,879 + EX28DRAFT_2997 0.23 -0.7 305,902 - EX28DRAFT_2997 0.27 -0.1 305,929 - EX28DRAFT_2997 0.30 -0.2 305,973 + EX28DRAFT_2997 0.37 -0.0 305,977 - EX28DRAFT_2997 0.37 +1.3 305,977 - EX28DRAFT_2997 0.37 +0.5 306,048 + EX28DRAFT_2997 0.47 -0.5
Or see this region's nucleotide sequence