Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2720 and EX28DRAFT_2721 are separated by 103 nucleotides EX28DRAFT_2721 and EX28DRAFT_2722 overlap by 11 nucleotides EX28DRAFT_2722 and EX28DRAFT_2723 are separated by 156 nucleotides EX28DRAFT_2723 and EX28DRAFT_2724 are separated by 89 nucleotides EX28DRAFT_2724 and EX28DRAFT_2725 are separated by 416 nucleotides
EX28DRAFT_2720: EX28DRAFT_2720 - Small protein A (tmRNA-binding), at 25,159 to 25,497
_2720
EX28DRAFT_2721: EX28DRAFT_2721 - Uncharacterized protein conserved in bacteria, at 25,601 to 25,888
_2721
EX28DRAFT_2722: EX28DRAFT_2722 - Oligoketide cyclase/lipid transport protein, at 25,878 to 26,315
_2722
EX28DRAFT_2723: EX28DRAFT_2723 - SsrA-binding protein, at 26,472 to 26,954
_2723
EX28DRAFT_2724: EX28DRAFT_2724 - RNA, at 27,044 to 27,407
_2724
EX28DRAFT_2725: EX28DRAFT_2725 - hypothetical protein, at 27,824 to 28,180
_2725
Position (kb) 26
27 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 25.495 kb on + strand at 25.496 kb on - strand at 25.602 kb on + strand at 25.602 kb on + strand at 25.603 kb on - strand at 25.606 kb on + strand at 25.837 kb on - strand, within EX28DRAFT_2721 at 25.858 kb on - strand, within EX28DRAFT_2721 at 25.863 kb on - strand at 25.863 kb on - strand at 25.868 kb on + strand at 25.868 kb on + strand at 25.891 kb on + strand at 26.350 kb on - strand at 26.356 kb on + strand at 26.356 kb on + strand at 26.357 kb on - strand at 26.394 kb on + strand at 26.399 kb on + strand at 26.472 kb on - strand at 26.634 kb on + strand, within EX28DRAFT_2723 at 26.634 kb on + strand, within EX28DRAFT_2723 at 26.710 kb on - strand, within EX28DRAFT_2723 at 27.025 kb on + strand at 27.025 kb on + strand at 27.026 kb on - strand at 27.164 kb on + strand, within EX28DRAFT_2724 at 27.344 kb on + strand, within EX28DRAFT_2724 at 27.344 kb on + strand, within EX28DRAFT_2724 at 27.618 kb on + strand at 27.618 kb on + strand at 27.619 kb on - strand at 27.619 kb on - strand at 27.638 kb on + strand at 27.638 kb on + strand at 27.638 kb on + strand at 27.639 kb on - strand at 27.639 kb on - strand at 27.729 kb on + strand at 27.810 kb on + strand at 27.811 kb on - strand at 27.821 kb on - strand at 27.823 kb on + strand at 27.824 kb on - strand at 27.838 kb on + strand at 27.839 kb on - strand at 27.846 kb on + strand at 27.847 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 25,495 + -5.5 25,496 - -1.0 25,602 + -2.1 25,602 + -3.1 25,603 - +0.3 25,606 + -0.9 25,837 - EX28DRAFT_2721 0.82 +0.8 25,858 - EX28DRAFT_2721 0.89 -0.9 25,863 - -0.4 25,863 - +0.0 25,868 + -1.6 25,868 + -0.8 25,891 + -0.3 26,350 - -0.6 26,356 + +1.4 26,356 + +0.7 26,357 - -1.5 26,394 + +0.2 26,399 + +0.4 26,472 - +4.3 26,634 + EX28DRAFT_2723 0.34 -1.1 26,634 + EX28DRAFT_2723 0.34 -1.8 26,710 - EX28DRAFT_2723 0.49 -1.8 27,025 + -1.5 27,025 + +0.6 27,026 - +0.2 27,164 + EX28DRAFT_2724 0.33 +0.6 27,344 + EX28DRAFT_2724 0.82 +1.1 27,344 + EX28DRAFT_2724 0.82 -1.3 27,618 + -1.5 27,618 + -0.2 27,619 - +2.5 27,619 - +0.9 27,638 + -0.0 27,638 + -2.0 27,638 + -1.1 27,639 - -1.2 27,639 - +0.3 27,729 + +1.7 27,810 + +0.5 27,811 - +0.5 27,821 - -0.1 27,823 + +1.0 27,824 - +0.1 27,838 + +3.1 27,839 - -0.8 27,846 + -0.9 27,847 - +1.5
Or see this region's nucleotide sequence