Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2358 and EX28DRAFT_2359 are separated by 104 nucleotides EX28DRAFT_2359 and EX28DRAFT_2360 are separated by 62 nucleotides
EX28DRAFT_2358: EX28DRAFT_2358 - S-methyl-5-thioribose-1-phosphate isomerase, at 2,377,779 to 2,378,795
_2358
EX28DRAFT_2359: EX28DRAFT_2359 - 5'-methylthioribose kinase (EC 2.7.1.100), at 2,378,900 to 2,380,099
_2359
EX28DRAFT_2360: EX28DRAFT_2360 - Uncharacterized conserved protein, at 2,380,162 to 2,381,403
_2360
Position (kb)
2378
2379
2380
2381 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2378.026 kb on + strand, within EX28DRAFT_2358 at 2378.027 kb on - strand, within EX28DRAFT_2358 at 2378.027 kb on - strand, within EX28DRAFT_2358 at 2378.069 kb on - strand, within EX28DRAFT_2358 at 2378.069 kb on - strand, within EX28DRAFT_2358 at 2378.228 kb on + strand, within EX28DRAFT_2358 at 2378.229 kb on - strand, within EX28DRAFT_2358 at 2378.351 kb on + strand, within EX28DRAFT_2358 at 2378.474 kb on - strand, within EX28DRAFT_2358 at 2378.474 kb on - strand, within EX28DRAFT_2358 at 2378.670 kb on - strand, within EX28DRAFT_2358 at 2378.744 kb on + strand at 2378.745 kb on - strand at 2378.801 kb on + strand at 2378.822 kb on + strand at 2378.822 kb on + strand at 2378.823 kb on - strand at 2378.853 kb on + strand at 2378.853 kb on + strand at 2378.853 kb on + strand at 2378.854 kb on - strand at 2378.855 kb on + strand at 2378.856 kb on - strand at 2378.856 kb on - strand at 2378.863 kb on + strand at 2378.872 kb on + strand at 2378.872 kb on + strand at 2378.909 kb on + strand at 2378.914 kb on + strand at 2378.915 kb on - strand at 2379.282 kb on - strand, within EX28DRAFT_2359 at 2379.282 kb on - strand, within EX28DRAFT_2359 at 2379.314 kb on + strand, within EX28DRAFT_2359 at 2379.315 kb on - strand, within EX28DRAFT_2359 at 2380.014 kb on + strand at 2380.025 kb on - strand at 2380.101 kb on + strand at 2380.101 kb on + strand at 2380.151 kb on + strand at 2380.197 kb on + strand at 2380.200 kb on - strand at 2380.215 kb on + strand at 2380.352 kb on + strand, within EX28DRAFT_2360 at 2380.352 kb on + strand, within EX28DRAFT_2360 at 2380.466 kb on + strand, within EX28DRAFT_2360 at 2380.467 kb on - strand, within EX28DRAFT_2360 at 2380.895 kb on + strand, within EX28DRAFT_2360 at 2380.896 kb on - strand, within EX28DRAFT_2360 at 2380.929 kb on - strand, within EX28DRAFT_2360 at 2381.023 kb on - strand, within EX28DRAFT_2360 at 2381.096 kb on + strand, within EX28DRAFT_2360 at 2381.096 kb on + strand, within EX28DRAFT_2360 at 2381.097 kb on - strand, within EX28DRAFT_2360
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,378,026 + EX28DRAFT_2358 0.24 -2.0 2,378,027 - EX28DRAFT_2358 0.24 +0.8 2,378,027 - EX28DRAFT_2358 0.24 -0.5 2,378,069 - EX28DRAFT_2358 0.29 +0.5 2,378,069 - EX28DRAFT_2358 0.29 -2.3 2,378,228 + EX28DRAFT_2358 0.44 -1.2 2,378,229 - EX28DRAFT_2358 0.44 -0.4 2,378,351 + EX28DRAFT_2358 0.56 +0.5 2,378,474 - EX28DRAFT_2358 0.68 +0.5 2,378,474 - EX28DRAFT_2358 0.68 -0.5 2,378,670 - EX28DRAFT_2358 0.88 -2.9 2,378,744 + +0.2 2,378,745 - +1.0 2,378,801 + -2.1 2,378,822 + -0.0 2,378,822 + +0.6 2,378,823 - +0.1 2,378,853 + -0.7 2,378,853 + +0.4 2,378,853 + -2.4 2,378,854 - +0.5 2,378,855 + +1.0 2,378,856 - +0.8 2,378,856 - +0.2 2,378,863 + -0.7 2,378,872 + +0.0 2,378,872 + -1.2 2,378,909 + -2.9 2,378,914 + +0.8 2,378,915 - -0.4 2,379,282 - EX28DRAFT_2359 0.32 -3.5 2,379,282 - EX28DRAFT_2359 0.32 -0.7 2,379,314 + EX28DRAFT_2359 0.34 -0.8 2,379,315 - EX28DRAFT_2359 0.35 -3.8 2,380,014 + +0.2 2,380,025 - -0.8 2,380,101 + -0.2 2,380,101 + +0.7 2,380,151 + +0.6 2,380,197 + -1.2 2,380,200 - +0.2 2,380,215 + +1.2 2,380,352 + EX28DRAFT_2360 0.15 -2.5 2,380,352 + EX28DRAFT_2360 0.15 +1.7 2,380,466 + EX28DRAFT_2360 0.24 +1.2 2,380,467 - EX28DRAFT_2360 0.25 +0.5 2,380,895 + EX28DRAFT_2360 0.59 +0.9 2,380,896 - EX28DRAFT_2360 0.59 +0.0 2,380,929 - EX28DRAFT_2360 0.62 -2.2 2,381,023 - EX28DRAFT_2360 0.69 +0.0 2,381,096 + EX28DRAFT_2360 0.75 +0.9 2,381,096 + EX28DRAFT_2360 0.75 +0.1 2,381,097 - EX28DRAFT_2360 0.75 -1.6
Or see this region's nucleotide sequence