Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2090 and EX28DRAFT_2091 are separated by 173 nucleotides EX28DRAFT_2091 and EX28DRAFT_2092 are separated by 80 nucleotides EX28DRAFT_2092 and EX28DRAFT_2093 overlap by 17 nucleotides EX28DRAFT_2093 and EX28DRAFT_2094 are separated by 116 nucleotides
EX28DRAFT_2090: EX28DRAFT_2090 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 2,114,218 to 2,114,886
_2090
EX28DRAFT_2091: EX28DRAFT_2091 - lysine-arginine-ornithine-binding periplasmic protein, at 2,115,060 to 2,115,791
_2091
EX28DRAFT_2092: EX28DRAFT_2092 - His Kinase A (phospho-acceptor) domain/Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase, at 2,115,872 to 2,117,341
_2092
EX28DRAFT_2093: EX28DRAFT_2093 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 2,117,325 to 2,118,032
_2093
EX28DRAFT_2094: EX28DRAFT_2094 - 23S rRNA (uracil-5-)-methyltransferase RumB, at 2,118,149 to 2,119,279
_2094
Position (kb)
2115
2116
2117
2118 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2114.901 kb on - strand at 2114.913 kb on - strand at 2115.008 kb on + strand at 2115.008 kb on + strand at 2115.009 kb on - strand at 2115.009 kb on - strand at 2115.038 kb on + strand at 2115.056 kb on + strand at 2115.056 kb on + strand at 2115.056 kb on + strand at 2115.056 kb on + strand at 2115.057 kb on - strand at 2115.070 kb on + strand at 2115.085 kb on + strand at 2115.369 kb on + strand, within EX28DRAFT_2091 at 2115.372 kb on + strand, within EX28DRAFT_2091 at 2115.372 kb on + strand, within EX28DRAFT_2091 at 2115.411 kb on + strand, within EX28DRAFT_2091 at 2115.412 kb on - strand, within EX28DRAFT_2091 at 2115.413 kb on + strand, within EX28DRAFT_2091 at 2115.413 kb on + strand, within EX28DRAFT_2091 at 2115.414 kb on - strand, within EX28DRAFT_2091 at 2115.414 kb on - strand, within EX28DRAFT_2091 at 2115.414 kb on - strand, within EX28DRAFT_2091 at 2115.525 kb on + strand, within EX28DRAFT_2091 at 2115.540 kb on - strand, within EX28DRAFT_2091 at 2115.658 kb on - strand, within EX28DRAFT_2091 at 2115.752 kb on + strand at 2115.752 kb on + strand at 2115.753 kb on - strand at 2115.753 kb on - strand at 2115.827 kb on + strand at 2115.828 kb on - strand at 2115.828 kb on - strand at 2115.828 kb on - strand at 2116.070 kb on - strand, within EX28DRAFT_2092 at 2116.077 kb on - strand, within EX28DRAFT_2092 at 2116.142 kb on - strand, within EX28DRAFT_2092 at 2116.331 kb on - strand, within EX28DRAFT_2092 at 2116.419 kb on + strand, within EX28DRAFT_2092 at 2116.755 kb on - strand, within EX28DRAFT_2092 at 2116.870 kb on + strand, within EX28DRAFT_2092 at 2116.871 kb on - strand, within EX28DRAFT_2092 at 2116.871 kb on - strand, within EX28DRAFT_2092 at 2116.875 kb on + strand, within EX28DRAFT_2092 at 2116.882 kb on - strand, within EX28DRAFT_2092 at 2116.933 kb on + strand, within EX28DRAFT_2092 at 2116.933 kb on + strand, within EX28DRAFT_2092 at 2117.079 kb on + strand, within EX28DRAFT_2092 at 2117.080 kb on - strand, within EX28DRAFT_2092 at 2117.080 kb on - strand, within EX28DRAFT_2092 at 2117.098 kb on + strand, within EX28DRAFT_2092 at 2117.183 kb on + strand, within EX28DRAFT_2092 at 2117.184 kb on - strand, within EX28DRAFT_2092 at 2117.362 kb on + strand at 2117.438 kb on + strand, within EX28DRAFT_2093 at 2117.438 kb on + strand, within EX28DRAFT_2093 at 2117.439 kb on - strand, within EX28DRAFT_2093 at 2117.447 kb on + strand, within EX28DRAFT_2093 at 2117.737 kb on + strand, within EX28DRAFT_2093 at 2118.056 kb on - strand at 2118.056 kb on - strand at 2118.056 kb on - strand at 2118.063 kb on - strand at 2118.063 kb on - strand at 2118.063 kb on - strand at 2118.063 kb on - strand at 2118.068 kb on + strand at 2118.193 kb on + strand at 2118.205 kb on + strand at 2118.288 kb on + strand, within EX28DRAFT_2094 at 2118.297 kb on + strand, within EX28DRAFT_2094 at 2118.321 kb on + strand, within EX28DRAFT_2094
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,114,901 - +0.5 2,114,913 - +0.2 2,115,008 + -3.1 2,115,008 + +1.6 2,115,009 - -0.3 2,115,009 - +0.1 2,115,038 + -4.6 2,115,056 + +0.3 2,115,056 + +0.4 2,115,056 + -0.2 2,115,056 + -1.3 2,115,057 - -0.4 2,115,070 + +0.4 2,115,085 + +0.1 2,115,369 + EX28DRAFT_2091 0.42 -0.3 2,115,372 + EX28DRAFT_2091 0.43 +0.4 2,115,372 + EX28DRAFT_2091 0.43 -0.2 2,115,411 + EX28DRAFT_2091 0.48 -2.9 2,115,412 - EX28DRAFT_2091 0.48 -0.2 2,115,413 + EX28DRAFT_2091 0.48 -2.9 2,115,413 + EX28DRAFT_2091 0.48 -1.8 2,115,414 - EX28DRAFT_2091 0.48 -0.4 2,115,414 - EX28DRAFT_2091 0.48 -1.2 2,115,414 - EX28DRAFT_2091 0.48 +1.2 2,115,525 + EX28DRAFT_2091 0.64 -0.0 2,115,540 - EX28DRAFT_2091 0.66 +1.1 2,115,658 - EX28DRAFT_2091 0.82 +1.1 2,115,752 + +0.7 2,115,752 + -0.4 2,115,753 - -3.2 2,115,753 - +1.3 2,115,827 + -0.3 2,115,828 - +0.4 2,115,828 - +0.4 2,115,828 - -0.2 2,116,070 - EX28DRAFT_2092 0.13 -0.3 2,116,077 - EX28DRAFT_2092 0.14 +0.4 2,116,142 - EX28DRAFT_2092 0.18 +0.5 2,116,331 - EX28DRAFT_2092 0.31 +2.4 2,116,419 + EX28DRAFT_2092 0.37 +1.4 2,116,755 - EX28DRAFT_2092 0.60 +0.7 2,116,870 + EX28DRAFT_2092 0.68 +0.6 2,116,871 - EX28DRAFT_2092 0.68 -3.1 2,116,871 - EX28DRAFT_2092 0.68 -0.0 2,116,875 + EX28DRAFT_2092 0.68 -0.0 2,116,882 - EX28DRAFT_2092 0.69 +0.6 2,116,933 + EX28DRAFT_2092 0.72 +0.5 2,116,933 + EX28DRAFT_2092 0.72 -2.3 2,117,079 + EX28DRAFT_2092 0.82 -0.2 2,117,080 - EX28DRAFT_2092 0.82 +0.3 2,117,080 - EX28DRAFT_2092 0.82 +0.2 2,117,098 + EX28DRAFT_2092 0.83 +1.0 2,117,183 + EX28DRAFT_2092 0.89 -5.6 2,117,184 - EX28DRAFT_2092 0.89 -0.1 2,117,362 + -1.0 2,117,438 + EX28DRAFT_2093 0.16 +0.1 2,117,438 + EX28DRAFT_2093 0.16 -0.5 2,117,439 - EX28DRAFT_2093 0.16 +1.0 2,117,447 + EX28DRAFT_2093 0.17 -0.1 2,117,737 + EX28DRAFT_2093 0.58 +1.3 2,118,056 - +0.4 2,118,056 - -0.6 2,118,056 - +0.2 2,118,063 - -0.6 2,118,063 - -1.3 2,118,063 - -1.1 2,118,063 - -0.6 2,118,068 + +1.2 2,118,193 + +0.1 2,118,205 + +0.1 2,118,288 + EX28DRAFT_2094 0.12 -1.0 2,118,297 + EX28DRAFT_2094 0.13 -0.2 2,118,321 + EX28DRAFT_2094 0.15 +0.3
Or see this region's nucleotide sequence