Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1981 and EX28DRAFT_1982 are separated by 123 nucleotides EX28DRAFT_1982 and EX28DRAFT_1983 are separated by 19 nucleotides
EX28DRAFT_1981: EX28DRAFT_1981 - Superfamily I DNA and RNA helicases, at 1,981,148 to 1,983,202
_1981
EX28DRAFT_1982: EX28DRAFT_1982 - Predicted membrane protein, at 1,983,326 to 1,983,772
_1982
EX28DRAFT_1983: EX28DRAFT_1983 - TIGR01666 family membrane protein, at 1,983,792 to 1,985,954
_1983
Position (kb)
1983
1984 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1982.359 kb on + strand, within EX28DRAFT_1981 at 1982.359 kb on + strand, within EX28DRAFT_1981 at 1982.360 kb on - strand, within EX28DRAFT_1981 at 1982.405 kb on - strand, within EX28DRAFT_1981 at 1982.632 kb on + strand, within EX28DRAFT_1981 at 1982.632 kb on + strand, within EX28DRAFT_1981 at 1982.701 kb on + strand, within EX28DRAFT_1981 at 1983.011 kb on - strand at 1983.091 kb on + strand at 1983.091 kb on + strand at 1983.091 kb on + strand at 1983.096 kb on + strand at 1983.097 kb on - strand at 1983.227 kb on - strand at 1983.227 kb on - strand at 1983.231 kb on + strand at 1983.256 kb on + strand at 1983.641 kb on - strand, within EX28DRAFT_1982 at 1983.775 kb on - strand at 1983.792 kb on - strand at 1983.818 kb on + strand at 1983.840 kb on + strand at 1983.840 kb on + strand at 1983.840 kb on + strand at 1983.840 kb on + strand at 1983.840 kb on + strand at 1983.841 kb on - strand at 1983.841 kb on - strand at 1983.853 kb on + strand at 1983.857 kb on + strand at 1984.076 kb on + strand, within EX28DRAFT_1983 at 1984.098 kb on - strand, within EX28DRAFT_1983 at 1984.098 kb on - strand, within EX28DRAFT_1983 at 1984.164 kb on + strand, within EX28DRAFT_1983 at 1984.164 kb on + strand, within EX28DRAFT_1983 at 1984.165 kb on - strand, within EX28DRAFT_1983 at 1984.165 kb on - strand, within EX28DRAFT_1983 at 1984.165 kb on - strand, within EX28DRAFT_1983 at 1984.239 kb on + strand, within EX28DRAFT_1983 at 1984.240 kb on - strand, within EX28DRAFT_1983 at 1984.240 kb on - strand, within EX28DRAFT_1983 at 1984.242 kb on - strand, within EX28DRAFT_1983 at 1984.393 kb on - strand, within EX28DRAFT_1983 at 1984.505 kb on - strand, within EX28DRAFT_1983 at 1984.505 kb on - strand, within EX28DRAFT_1983 at 1984.505 kb on - strand, within EX28DRAFT_1983 at 1984.510 kb on - strand, within EX28DRAFT_1983 at 1984.510 kb on - strand, within EX28DRAFT_1983 at 1984.513 kb on - strand, within EX28DRAFT_1983 at 1984.677 kb on - strand, within EX28DRAFT_1983
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,982,359 + EX28DRAFT_1981 0.59 +2.5 1,982,359 + EX28DRAFT_1981 0.59 +0.2 1,982,360 - EX28DRAFT_1981 0.59 +0.6 1,982,405 - EX28DRAFT_1981 0.61 +0.3 1,982,632 + EX28DRAFT_1981 0.72 -0.6 1,982,632 + EX28DRAFT_1981 0.72 -2.9 1,982,701 + EX28DRAFT_1981 0.76 +0.7 1,983,011 - -0.6 1,983,091 + +1.0 1,983,091 + -0.5 1,983,091 + +1.1 1,983,096 + -1.8 1,983,097 - -3.3 1,983,227 - -0.6 1,983,227 - +1.8 1,983,231 + +0.3 1,983,256 + -0.2 1,983,641 - EX28DRAFT_1982 0.70 +0.3 1,983,775 - +1.1 1,983,792 - -1.1 1,983,818 + -0.5 1,983,840 + -0.1 1,983,840 + +0.1 1,983,840 + -2.5 1,983,840 + +0.4 1,983,840 + +0.7 1,983,841 - +1.2 1,983,841 - -0.0 1,983,853 + +0.2 1,983,857 + -0.5 1,984,076 + EX28DRAFT_1983 0.13 -2.4 1,984,098 - EX28DRAFT_1983 0.14 +0.4 1,984,098 - EX28DRAFT_1983 0.14 +0.2 1,984,164 + EX28DRAFT_1983 0.17 -0.1 1,984,164 + EX28DRAFT_1983 0.17 +0.7 1,984,165 - EX28DRAFT_1983 0.17 -3.5 1,984,165 - EX28DRAFT_1983 0.17 -0.3 1,984,165 - EX28DRAFT_1983 0.17 -0.6 1,984,239 + EX28DRAFT_1983 0.21 -0.1 1,984,240 - EX28DRAFT_1983 0.21 +0.5 1,984,240 - EX28DRAFT_1983 0.21 -0.7 1,984,242 - EX28DRAFT_1983 0.21 -1.2 1,984,393 - EX28DRAFT_1983 0.28 +0.8 1,984,505 - EX28DRAFT_1983 0.33 -0.8 1,984,505 - EX28DRAFT_1983 0.33 -0.8 1,984,505 - EX28DRAFT_1983 0.33 +0.4 1,984,510 - EX28DRAFT_1983 0.33 -0.5 1,984,510 - EX28DRAFT_1983 0.33 +0.4 1,984,513 - EX28DRAFT_1983 0.33 +1.5 1,984,677 - EX28DRAFT_1983 0.41 +0.1
Or see this region's nucleotide sequence