Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1532 and EX28DRAFT_1533 are separated by 27 nucleotides EX28DRAFT_1533 and EX28DRAFT_1534 are separated by 214 nucleotides EX28DRAFT_1534 and EX28DRAFT_1535 overlap by 20 nucleotides EX28DRAFT_1535 and EX28DRAFT_1536 are separated by 175 nucleotides
EX28DRAFT_1532: EX28DRAFT_1532 - methyl-accepting chemotaxis sensory transducer with TarH sensor, at 1,546,644 to 1,548,305
_1532
EX28DRAFT_1533: EX28DRAFT_1533 - Uncharacterized protein conserved in bacteria, at 1,548,333 to 1,548,896
_1533
EX28DRAFT_1534: EX28DRAFT_1534 - RNA, at 1,549,111 to 1,549,270
_1534
EX28DRAFT_1535: EX28DRAFT_1535 - Transcriptional regulator, at 1,549,251 to 1,550,162
_1535
EX28DRAFT_1536: EX28DRAFT_1536 - amidohydrolase, at 1,550,338 to 1,551,648
_1536
Position (kb)
1549
1550
1551 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1548.403 kb on + strand, within EX28DRAFT_1533 at 1548.404 kb on - strand, within EX28DRAFT_1533 at 1548.404 kb on - strand, within EX28DRAFT_1533 at 1548.404 kb on - strand, within EX28DRAFT_1533 at 1548.582 kb on - strand, within EX28DRAFT_1533 at 1548.589 kb on + strand, within EX28DRAFT_1533 at 1548.652 kb on + strand, within EX28DRAFT_1533 at 1548.652 kb on + strand, within EX28DRAFT_1533 at 1548.800 kb on + strand, within EX28DRAFT_1533 at 1548.801 kb on - strand, within EX28DRAFT_1533 at 1548.926 kb on + strand at 1548.927 kb on - strand at 1548.927 kb on - strand at 1548.929 kb on + strand at 1548.930 kb on - strand at 1548.932 kb on - strand at 1548.932 kb on - strand at 1549.001 kb on + strand at 1549.003 kb on + strand at 1549.004 kb on - strand at 1549.004 kb on - strand at 1549.004 kb on - strand at 1549.004 kb on - strand at 1549.006 kb on - strand at 1549.011 kb on - strand at 1549.014 kb on + strand at 1549.089 kb on + strand at 1549.134 kb on + strand, within EX28DRAFT_1534 at 1549.139 kb on - strand, within EX28DRAFT_1534 at 1549.142 kb on + strand, within EX28DRAFT_1534 at 1549.143 kb on - strand, within EX28DRAFT_1534 at 1549.202 kb on + strand, within EX28DRAFT_1534 at 1549.202 kb on + strand, within EX28DRAFT_1534 at 1549.292 kb on + strand at 1549.341 kb on + strand at 1549.346 kb on - strand, within EX28DRAFT_1535 at 1549.348 kb on + strand, within EX28DRAFT_1535 at 1549.348 kb on + strand, within EX28DRAFT_1535 at 1549.434 kb on + strand, within EX28DRAFT_1535 at 1549.469 kb on + strand, within EX28DRAFT_1535 at 1549.549 kb on + strand, within EX28DRAFT_1535 at 1549.552 kb on + strand, within EX28DRAFT_1535 at 1549.553 kb on - strand, within EX28DRAFT_1535 at 1549.585 kb on + strand, within EX28DRAFT_1535 at 1549.602 kb on - strand, within EX28DRAFT_1535 at 1549.708 kb on + strand, within EX28DRAFT_1535 at 1549.708 kb on + strand, within EX28DRAFT_1535 at 1549.709 kb on - strand, within EX28DRAFT_1535 at 1549.743 kb on + strand, within EX28DRAFT_1535 at 1549.958 kb on - strand, within EX28DRAFT_1535 at 1550.163 kb on - strand at 1550.209 kb on + strand at 1550.210 kb on - strand at 1550.210 kb on - strand at 1550.215 kb on - strand at 1550.279 kb on - strand at 1550.283 kb on - strand at 1550.313 kb on + strand at 1550.314 kb on - strand at 1550.944 kb on + strand, within EX28DRAFT_1536 at 1550.961 kb on + strand, within EX28DRAFT_1536 at 1550.961 kb on + strand, within EX28DRAFT_1536 at 1550.961 kb on + strand, within EX28DRAFT_1536 at 1550.961 kb on + strand, within EX28DRAFT_1536
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,548,403 + EX28DRAFT_1533 0.12 +0.3 1,548,404 - EX28DRAFT_1533 0.13 +0.7 1,548,404 - EX28DRAFT_1533 0.13 -2.9 1,548,404 - EX28DRAFT_1533 0.13 +0.8 1,548,582 - EX28DRAFT_1533 0.44 -2.1 1,548,589 + EX28DRAFT_1533 0.45 +0.7 1,548,652 + EX28DRAFT_1533 0.57 +1.3 1,548,652 + EX28DRAFT_1533 0.57 -0.7 1,548,800 + EX28DRAFT_1533 0.83 +1.1 1,548,801 - EX28DRAFT_1533 0.83 +1.0 1,548,926 + -1.1 1,548,927 - -0.0 1,548,927 - -1.1 1,548,929 + -1.1 1,548,930 - -0.1 1,548,932 - +1.2 1,548,932 - -0.2 1,549,001 + +0.6 1,549,003 + +0.6 1,549,004 - -0.5 1,549,004 - +1.0 1,549,004 - -0.7 1,549,004 - +1.6 1,549,006 - -1.1 1,549,011 - -0.4 1,549,014 + +1.5 1,549,089 + -0.9 1,549,134 + EX28DRAFT_1534 0.14 -0.5 1,549,139 - EX28DRAFT_1534 0.17 -0.6 1,549,142 + EX28DRAFT_1534 0.19 +1.3 1,549,143 - EX28DRAFT_1534 0.20 -0.3 1,549,202 + EX28DRAFT_1534 0.57 +0.3 1,549,202 + EX28DRAFT_1534 0.57 -1.2 1,549,292 + +0.2 1,549,341 + +0.1 1,549,346 - EX28DRAFT_1535 0.10 +0.5 1,549,348 + EX28DRAFT_1535 0.11 +0.2 1,549,348 + EX28DRAFT_1535 0.11 -1.5 1,549,434 + EX28DRAFT_1535 0.20 -0.1 1,549,469 + EX28DRAFT_1535 0.24 -1.1 1,549,549 + EX28DRAFT_1535 0.33 -0.3 1,549,552 + EX28DRAFT_1535 0.33 +0.7 1,549,553 - EX28DRAFT_1535 0.33 -1.6 1,549,585 + EX28DRAFT_1535 0.37 -0.1 1,549,602 - EX28DRAFT_1535 0.38 -1.4 1,549,708 + EX28DRAFT_1535 0.50 +0.5 1,549,708 + EX28DRAFT_1535 0.50 -1.1 1,549,709 - EX28DRAFT_1535 0.50 -0.3 1,549,743 + EX28DRAFT_1535 0.54 -0.4 1,549,958 - EX28DRAFT_1535 0.78 +0.3 1,550,163 - +1.7 1,550,209 + +0.2 1,550,210 - -0.6 1,550,210 - +0.4 1,550,215 - +2.0 1,550,279 - -2.9 1,550,283 - -1.1 1,550,313 + +0.7 1,550,314 - +1.2 1,550,944 + EX28DRAFT_1536 0.46 -0.5 1,550,961 + EX28DRAFT_1536 0.48 -2.6 1,550,961 + EX28DRAFT_1536 0.48 -4.0 1,550,961 + EX28DRAFT_1536 0.48 -2.0 1,550,961 + EX28DRAFT_1536 0.48 +0.2
Or see this region's nucleotide sequence