Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1365 and EX28DRAFT_1366 are separated by 95 nucleotides EX28DRAFT_1366 and EX28DRAFT_1367 are separated by 4 nucleotides EX28DRAFT_1367 and EX28DRAFT_1368 overlap by 4 nucleotides EX28DRAFT_1368 and EX28DRAFT_1369 overlap by 4 nucleotides
EX28DRAFT_1365: EX28DRAFT_1365 - Transcriptional regulator, at 1,383,209 to 1,384,141
_1365
EX28DRAFT_1366: EX28DRAFT_1366 - Uncharacterized protein conserved in bacteria, at 1,384,237 to 1,384,671
_1366
EX28DRAFT_1367: EX28DRAFT_1367 - Uncharacterized protein conserved in bacteria, at 1,384,676 to 1,385,134
_1367
EX28DRAFT_1368: EX28DRAFT_1368 - Protein of unknown function (DUF3833), at 1,385,131 to 1,385,664
_1368
EX28DRAFT_1369: EX28DRAFT_1369 - hypothetical protein, at 1,385,661 to 1,386,182
_1369
Position (kb)
1384
1385
1386 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1383.758 kb on + strand, within EX28DRAFT_1365 at 1383.978 kb on + strand, within EX28DRAFT_1365 at 1383.979 kb on - strand, within EX28DRAFT_1365 at 1384.204 kb on + strand at 1384.471 kb on + strand, within EX28DRAFT_1366 at 1384.617 kb on + strand, within EX28DRAFT_1366 at 1384.618 kb on - strand, within EX28DRAFT_1366 at 1384.636 kb on - strand at 1384.676 kb on - strand at 1384.686 kb on + strand at 1384.687 kb on - strand at 1384.843 kb on + strand, within EX28DRAFT_1367 at 1385.090 kb on + strand at 1385.091 kb on - strand at 1385.099 kb on + strand at 1385.100 kb on - strand at 1385.235 kb on - strand, within EX28DRAFT_1368 at 1385.294 kb on + strand, within EX28DRAFT_1368 at 1385.367 kb on - strand, within EX28DRAFT_1368 at 1385.389 kb on + strand, within EX28DRAFT_1368 at 1385.390 kb on - strand, within EX28DRAFT_1368 at 1385.390 kb on - strand, within EX28DRAFT_1368 at 1385.391 kb on + strand, within EX28DRAFT_1368 at 1385.610 kb on + strand, within EX28DRAFT_1368 at 1385.778 kb on - strand, within EX28DRAFT_1369 at 1385.992 kb on + strand, within EX28DRAFT_1369 at 1385.992 kb on + strand, within EX28DRAFT_1369 at 1385.993 kb on - strand, within EX28DRAFT_1369 at 1385.997 kb on - strand, within EX28DRAFT_1369 at 1385.997 kb on - strand, within EX28DRAFT_1369 at 1386.000 kb on - strand, within EX28DRAFT_1369 at 1386.000 kb on - strand, within EX28DRAFT_1369 at 1386.048 kb on + strand, within EX28DRAFT_1369 at 1386.059 kb on + strand, within EX28DRAFT_1369 at 1386.059 kb on + strand, within EX28DRAFT_1369 at 1386.059 kb on + strand, within EX28DRAFT_1369 at 1386.060 kb on - strand, within EX28DRAFT_1369 at 1386.061 kb on + strand, within EX28DRAFT_1369 at 1386.062 kb on - strand, within EX28DRAFT_1369
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,383,758 + EX28DRAFT_1365 0.59 -0.2 1,383,978 + EX28DRAFT_1365 0.82 -2.7 1,383,979 - EX28DRAFT_1365 0.83 +0.8 1,384,204 + +0.3 1,384,471 + EX28DRAFT_1366 0.54 +0.1 1,384,617 + EX28DRAFT_1366 0.87 +0.5 1,384,618 - EX28DRAFT_1366 0.88 +0.0 1,384,636 - +0.3 1,384,676 - -0.2 1,384,686 + -0.2 1,384,687 - +0.5 1,384,843 + EX28DRAFT_1367 0.36 -1.7 1,385,090 + -0.2 1,385,091 - -2.2 1,385,099 + +2.6 1,385,100 - -1.2 1,385,235 - EX28DRAFT_1368 0.19 -0.5 1,385,294 + EX28DRAFT_1368 0.31 +0.2 1,385,367 - EX28DRAFT_1368 0.44 -2.5 1,385,389 + EX28DRAFT_1368 0.48 +0.3 1,385,390 - EX28DRAFT_1368 0.49 +0.0 1,385,390 - EX28DRAFT_1368 0.49 +0.4 1,385,391 + EX28DRAFT_1368 0.49 +0.1 1,385,610 + EX28DRAFT_1368 0.90 -0.5 1,385,778 - EX28DRAFT_1369 0.22 +0.9 1,385,992 + EX28DRAFT_1369 0.63 -0.9 1,385,992 + EX28DRAFT_1369 0.63 -0.9 1,385,993 - EX28DRAFT_1369 0.64 -2.7 1,385,997 - EX28DRAFT_1369 0.64 -1.2 1,385,997 - EX28DRAFT_1369 0.64 +1.3 1,386,000 - EX28DRAFT_1369 0.65 +0.1 1,386,000 - EX28DRAFT_1369 0.65 +0.1 1,386,048 + EX28DRAFT_1369 0.74 -0.5 1,386,059 + EX28DRAFT_1369 0.76 -0.2 1,386,059 + EX28DRAFT_1369 0.76 -0.5 1,386,059 + EX28DRAFT_1369 0.76 +1.2 1,386,060 - EX28DRAFT_1369 0.76 -2.5 1,386,061 + EX28DRAFT_1369 0.77 -0.2 1,386,062 - EX28DRAFT_1369 0.77 +0.4
Or see this region's nucleotide sequence