Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1348 and EX28DRAFT_1349 are separated by 45 nucleotides EX28DRAFT_1349 and EX28DRAFT_1350 are separated by 189 nucleotides EX28DRAFT_1350 and EX28DRAFT_1351 are separated by 53 nucleotides
EX28DRAFT_1348: EX28DRAFT_1348 - Predicted dehydrogenases and related proteins, at 1,362,316 to 1,363,383
_1348
EX28DRAFT_1349: EX28DRAFT_1349 - Cytochrome B561, at 1,363,429 to 1,363,959
_1349
EX28DRAFT_1350: EX28DRAFT_1350 - glyceraldehyde-3-phosphate dehydrogenase, type I, at 1,364,149 to 1,365,150
_1350
EX28DRAFT_1351: EX28DRAFT_1351 - Uncharacterized conserved protein, at 1,365,204 to 1,365,998
_1351
Position (kb)
1364
1365
1366 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1363.216 kb on + strand, within EX28DRAFT_1348 at 1363.219 kb on + strand, within EX28DRAFT_1348 at 1363.219 kb on + strand, within EX28DRAFT_1348 at 1363.219 kb on + strand, within EX28DRAFT_1348 at 1363.220 kb on - strand, within EX28DRAFT_1348 at 1363.220 kb on - strand, within EX28DRAFT_1348 at 1363.230 kb on - strand, within EX28DRAFT_1348 at 1363.381 kb on + strand at 1363.430 kb on + strand at 1363.467 kb on + strand at 1363.467 kb on + strand at 1363.481 kb on + strand at 1363.482 kb on - strand, within EX28DRAFT_1349 at 1363.482 kb on - strand, within EX28DRAFT_1349 at 1363.482 kb on - strand, within EX28DRAFT_1349 at 1363.482 kb on - strand, within EX28DRAFT_1349 at 1363.644 kb on - strand, within EX28DRAFT_1349 at 1363.644 kb on - strand, within EX28DRAFT_1349 at 1363.644 kb on - strand, within EX28DRAFT_1349 at 1363.644 kb on - strand, within EX28DRAFT_1349 at 1363.644 kb on - strand, within EX28DRAFT_1349 at 1363.678 kb on - strand, within EX28DRAFT_1349 at 1363.689 kb on - strand, within EX28DRAFT_1349 at 1363.834 kb on - strand, within EX28DRAFT_1349 at 1363.834 kb on - strand, within EX28DRAFT_1349 at 1363.890 kb on - strand, within EX28DRAFT_1349 at 1363.890 kb on - strand, within EX28DRAFT_1349 at 1363.944 kb on + strand at 1363.945 kb on - strand at 1363.945 kb on - strand at 1363.946 kb on + strand at 1363.996 kb on + strand at 1364.136 kb on - strand at 1364.195 kb on + strand at 1364.299 kb on + strand, within EX28DRAFT_1350 at 1364.299 kb on + strand, within EX28DRAFT_1350 at 1364.299 kb on + strand, within EX28DRAFT_1350 at 1364.299 kb on + strand, within EX28DRAFT_1350 at 1364.417 kb on + strand, within EX28DRAFT_1350 at 1364.417 kb on + strand, within EX28DRAFT_1350 at 1364.422 kb on - strand, within EX28DRAFT_1350 at 1364.446 kb on + strand, within EX28DRAFT_1350 at 1364.448 kb on + strand, within EX28DRAFT_1350 at 1364.580 kb on + strand, within EX28DRAFT_1350 at 1364.580 kb on + strand, within EX28DRAFT_1350 at 1364.581 kb on - strand, within EX28DRAFT_1350 at 1364.581 kb on - strand, within EX28DRAFT_1350 at 1364.684 kb on - strand, within EX28DRAFT_1350 at 1364.758 kb on - strand, within EX28DRAFT_1350 at 1364.758 kb on - strand, within EX28DRAFT_1350 at 1364.949 kb on + strand, within EX28DRAFT_1350 at 1364.949 kb on + strand, within EX28DRAFT_1350 at 1364.950 kb on - strand, within EX28DRAFT_1350 at 1364.962 kb on + strand, within EX28DRAFT_1350 at 1365.097 kb on + strand at 1365.206 kb on - strand at 1365.211 kb on - strand at 1365.627 kb on - strand, within EX28DRAFT_1351 at 1365.760 kb on + strand, within EX28DRAFT_1351 at 1365.761 kb on - strand, within EX28DRAFT_1351 at 1365.945 kb on - strand at 1366.149 kb on + strand at 1366.149 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,363,216 + EX28DRAFT_1348 0.84 -0.5 1,363,219 + EX28DRAFT_1348 0.85 +0.5 1,363,219 + EX28DRAFT_1348 0.85 -1.2 1,363,219 + EX28DRAFT_1348 0.85 -2.0 1,363,220 - EX28DRAFT_1348 0.85 -2.2 1,363,220 - EX28DRAFT_1348 0.85 -1.4 1,363,230 - EX28DRAFT_1348 0.86 -1.7 1,363,381 + -4.1 1,363,430 + +1.8 1,363,467 + +1.3 1,363,467 + -0.5 1,363,481 + -0.8 1,363,482 - EX28DRAFT_1349 0.10 -0.1 1,363,482 - EX28DRAFT_1349 0.10 -1.1 1,363,482 - EX28DRAFT_1349 0.10 -0.3 1,363,482 - EX28DRAFT_1349 0.10 -0.0 1,363,644 - EX28DRAFT_1349 0.40 +1.2 1,363,644 - EX28DRAFT_1349 0.40 -0.1 1,363,644 - EX28DRAFT_1349 0.40 -0.6 1,363,644 - EX28DRAFT_1349 0.40 +0.5 1,363,644 - EX28DRAFT_1349 0.40 -0.5 1,363,678 - EX28DRAFT_1349 0.47 +1.5 1,363,689 - EX28DRAFT_1349 0.49 -1.1 1,363,834 - EX28DRAFT_1349 0.76 +0.6 1,363,834 - EX28DRAFT_1349 0.76 -0.9 1,363,890 - EX28DRAFT_1349 0.87 -1.7 1,363,890 - EX28DRAFT_1349 0.87 +0.1 1,363,944 + +0.4 1,363,945 - +1.5 1,363,945 - +1.1 1,363,946 + +0.4 1,363,996 + -0.8 1,364,136 - -0.8 1,364,195 + +0.3 1,364,299 + EX28DRAFT_1350 0.15 +0.2 1,364,299 + EX28DRAFT_1350 0.15 +0.5 1,364,299 + EX28DRAFT_1350 0.15 -0.3 1,364,299 + EX28DRAFT_1350 0.15 -0.2 1,364,417 + EX28DRAFT_1350 0.27 -2.7 1,364,417 + EX28DRAFT_1350 0.27 -0.2 1,364,422 - EX28DRAFT_1350 0.27 -0.2 1,364,446 + EX28DRAFT_1350 0.30 -1.1 1,364,448 + EX28DRAFT_1350 0.30 -0.0 1,364,580 + EX28DRAFT_1350 0.43 -0.2 1,364,580 + EX28DRAFT_1350 0.43 -1.4 1,364,581 - EX28DRAFT_1350 0.43 -0.3 1,364,581 - EX28DRAFT_1350 0.43 -0.7 1,364,684 - EX28DRAFT_1350 0.53 +0.6 1,364,758 - EX28DRAFT_1350 0.61 -0.4 1,364,758 - EX28DRAFT_1350 0.61 +0.2 1,364,949 + EX28DRAFT_1350 0.80 -1.2 1,364,949 + EX28DRAFT_1350 0.80 +0.9 1,364,950 - EX28DRAFT_1350 0.80 +0.3 1,364,962 + EX28DRAFT_1350 0.81 +0.3 1,365,097 + +0.8 1,365,206 - -3.0 1,365,211 - +1.4 1,365,627 - EX28DRAFT_1351 0.53 -0.1 1,365,760 + EX28DRAFT_1351 0.70 +2.6 1,365,761 - EX28DRAFT_1351 0.70 +0.3 1,365,945 - +2.3 1,366,149 + -0.2 1,366,149 + +2.2
Or see this region's nucleotide sequence