Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1320 and EX28DRAFT_1321 are separated by 3 nucleotides EX28DRAFT_1321 and EX28DRAFT_1322 are separated by 11 nucleotides EX28DRAFT_1322 and EX28DRAFT_1323 are separated by 11 nucleotides EX28DRAFT_1323 and EX28DRAFT_1324 overlap by 4 nucleotides EX28DRAFT_1324 and EX28DRAFT_1325 overlap by 4 nucleotides
EX28DRAFT_1320: EX28DRAFT_1320 - DNA polymerase III, epsilon subunit and related 3'-5' exonucleases, at 1,320,534 to 1,321,190
_1320
EX28DRAFT_1321: EX28DRAFT_1321 - hypothetical protein, at 1,321,194 to 1,321,859
_1321
EX28DRAFT_1322: EX28DRAFT_1322 - hypothetical protein, at 1,321,871 to 1,322,581
_1322
EX28DRAFT_1323: EX28DRAFT_1323 - Protein of unknown function (DUF1317), at 1,322,593 to 1,322,745
_1323
EX28DRAFT_1324: EX28DRAFT_1324 - Methyltransferase domain, at 1,322,742 to 1,323,224
_1324
EX28DRAFT_1325: EX28DRAFT_1325 - Transcriptional activator, adenine-specific DNA methyltransferase, at 1,323,221 to 1,323,880
_1325
Position (kb) 1321
1322
1323 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1320.882 kb on + strand, within EX28DRAFT_1320 at 1320.958 kb on - strand, within EX28DRAFT_1320 at 1320.982 kb on - strand, within EX28DRAFT_1320 at 1321.074 kb on - strand, within EX28DRAFT_1320 at 1321.188 kb on + strand at 1321.242 kb on + strand at 1321.243 kb on - strand at 1321.275 kb on - strand, within EX28DRAFT_1321 at 1321.444 kb on + strand, within EX28DRAFT_1321 at 1321.445 kb on - strand, within EX28DRAFT_1321 at 1321.448 kb on + strand, within EX28DRAFT_1321 at 1321.448 kb on + strand, within EX28DRAFT_1321 at 1321.449 kb on - strand, within EX28DRAFT_1321 at 1321.449 kb on - strand, within EX28DRAFT_1321 at 1321.449 kb on - strand, within EX28DRAFT_1321 at 1321.449 kb on - strand, within EX28DRAFT_1321 at 1321.512 kb on + strand, within EX28DRAFT_1321 at 1321.555 kb on + strand, within EX28DRAFT_1321 at 1321.556 kb on - strand, within EX28DRAFT_1321 at 1321.556 kb on - strand, within EX28DRAFT_1321 at 1321.574 kb on - strand, within EX28DRAFT_1321 at 1321.574 kb on - strand, within EX28DRAFT_1321 at 1321.574 kb on - strand, within EX28DRAFT_1321 at 1321.594 kb on - strand, within EX28DRAFT_1321 at 1321.857 kb on + strand at 1321.857 kb on + strand at 1321.858 kb on - strand at 1321.892 kb on - strand at 1321.965 kb on - strand, within EX28DRAFT_1322 at 1321.965 kb on - strand, within EX28DRAFT_1322 at 1321.991 kb on + strand, within EX28DRAFT_1322 at 1321.991 kb on + strand, within EX28DRAFT_1322 at 1321.992 kb on - strand, within EX28DRAFT_1322 at 1322.222 kb on - strand, within EX28DRAFT_1322 at 1322.282 kb on + strand, within EX28DRAFT_1322 at 1322.282 kb on + strand, within EX28DRAFT_1322 at 1322.330 kb on + strand, within EX28DRAFT_1322 at 1322.331 kb on - strand, within EX28DRAFT_1322 at 1322.332 kb on + strand, within EX28DRAFT_1322 at 1322.474 kb on - strand, within EX28DRAFT_1322 at 1322.569 kb on + strand at 1322.592 kb on + strand at 1322.593 kb on - strand at 1322.649 kb on + strand, within EX28DRAFT_1323 at 1322.875 kb on + strand, within EX28DRAFT_1324 at 1322.888 kb on + strand, within EX28DRAFT_1324 at 1322.889 kb on - strand, within EX28DRAFT_1324 at 1322.890 kb on + strand, within EX28DRAFT_1324 at 1323.125 kb on + strand, within EX28DRAFT_1324 at 1323.158 kb on - strand, within EX28DRAFT_1324 at 1323.241 kb on + strand at 1323.241 kb on + strand at 1323.245 kb on + strand at 1323.246 kb on - strand at 1323.267 kb on - strand at 1323.386 kb on + strand, within EX28DRAFT_1325
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,320,882 + EX28DRAFT_1320 0.53 -1.4 1,320,958 - EX28DRAFT_1320 0.65 +1.2 1,320,982 - EX28DRAFT_1320 0.68 -0.1 1,321,074 - EX28DRAFT_1320 0.82 -3.5 1,321,188 + +0.9 1,321,242 + +0.3 1,321,243 - +1.3 1,321,275 - EX28DRAFT_1321 0.12 -0.0 1,321,444 + EX28DRAFT_1321 0.38 -0.4 1,321,445 - EX28DRAFT_1321 0.38 +0.6 1,321,448 + EX28DRAFT_1321 0.38 +2.4 1,321,448 + EX28DRAFT_1321 0.38 -2.8 1,321,449 - EX28DRAFT_1321 0.38 -3.9 1,321,449 - EX28DRAFT_1321 0.38 +0.7 1,321,449 - EX28DRAFT_1321 0.38 -3.3 1,321,449 - EX28DRAFT_1321 0.38 -3.8 1,321,512 + EX28DRAFT_1321 0.48 -0.2 1,321,555 + EX28DRAFT_1321 0.54 +1.0 1,321,556 - EX28DRAFT_1321 0.54 +0.2 1,321,556 - EX28DRAFT_1321 0.54 -1.9 1,321,574 - EX28DRAFT_1321 0.57 -0.3 1,321,574 - EX28DRAFT_1321 0.57 +1.1 1,321,574 - EX28DRAFT_1321 0.57 -0.7 1,321,594 - EX28DRAFT_1321 0.60 +0.1 1,321,857 + +0.1 1,321,857 + -0.9 1,321,858 - -0.2 1,321,892 - +0.5 1,321,965 - EX28DRAFT_1322 0.13 -0.6 1,321,965 - EX28DRAFT_1322 0.13 -1.3 1,321,991 + EX28DRAFT_1322 0.17 +0.3 1,321,991 + EX28DRAFT_1322 0.17 +1.9 1,321,992 - EX28DRAFT_1322 0.17 +1.4 1,322,222 - EX28DRAFT_1322 0.49 +0.4 1,322,282 + EX28DRAFT_1322 0.58 +0.1 1,322,282 + EX28DRAFT_1322 0.58 -0.4 1,322,330 + EX28DRAFT_1322 0.65 -0.9 1,322,331 - EX28DRAFT_1322 0.65 -1.9 1,322,332 + EX28DRAFT_1322 0.65 +0.8 1,322,474 - EX28DRAFT_1322 0.85 +0.6 1,322,569 + +0.1 1,322,592 + +3.0 1,322,593 - +1.0 1,322,649 + EX28DRAFT_1323 0.37 -3.2 1,322,875 + EX28DRAFT_1324 0.28 +1.2 1,322,888 + EX28DRAFT_1324 0.30 -0.8 1,322,889 - EX28DRAFT_1324 0.30 -1.5 1,322,890 + EX28DRAFT_1324 0.31 +0.4 1,323,125 + EX28DRAFT_1324 0.79 +1.4 1,323,158 - EX28DRAFT_1324 0.86 -0.5 1,323,241 + -1.5 1,323,241 + -0.2 1,323,245 + +0.7 1,323,246 - -0.6 1,323,267 - -0.3 1,323,386 + EX28DRAFT_1325 0.25 +0.6
Or see this region's nucleotide sequence