Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1299 and EX28DRAFT_1300 overlap by 4 nucleotides EX28DRAFT_1300 and EX28DRAFT_1301 overlap by 4 nucleotides EX28DRAFT_1301 and EX28DRAFT_1302 overlap by 4 nucleotides EX28DRAFT_1302 and EX28DRAFT_1303 overlap by 8 nucleotides EX28DRAFT_1303 and EX28DRAFT_1304 overlap by 8 nucleotides EX28DRAFT_1304 and EX28DRAFT_1305 are separated by 260 nucleotides
EX28DRAFT_1299: EX28DRAFT_1299 - Phage antitermination protein Q, at 1,309,434 to 1,310,123
_1299
EX28DRAFT_1300: EX28DRAFT_1300 - hypothetical protein, at 1,310,120 to 1,310,260
_1300
EX28DRAFT_1301: EX28DRAFT_1301 - hypothetical protein, at 1,310,257 to 1,310,481
_1301
EX28DRAFT_1302: EX28DRAFT_1302 - Bacteriophage Lambda NinG protein, at 1,310,478 to 1,311,089
_1302
EX28DRAFT_1303: EX28DRAFT_1303 - hypothetical protein, at 1,311,082 to 1,311,252
_1303
EX28DRAFT_1304: EX28DRAFT_1304 - NinB protein, at 1,311,245 to 1,311,694
_1304
EX28DRAFT_1305: EX28DRAFT_1305 - hypothetical protein, at 1,311,955 to 1,312,755
_1305
Position (kb) 1311
1312 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1310.101 kb on + strand at 1310.146 kb on - strand, within EX28DRAFT_1300 at 1310.238 kb on - strand, within EX28DRAFT_1300 at 1310.285 kb on + strand, within EX28DRAFT_1301 at 1310.286 kb on - strand, within EX28DRAFT_1301 at 1310.286 kb on - strand, within EX28DRAFT_1301 at 1310.286 kb on - strand, within EX28DRAFT_1301 at 1310.294 kb on + strand, within EX28DRAFT_1301 at 1310.400 kb on + strand, within EX28DRAFT_1301 at 1310.461 kb on + strand at 1310.462 kb on - strand at 1310.462 kb on - strand at 1310.465 kb on + strand at 1310.466 kb on - strand at 1310.466 kb on - strand at 1310.475 kb on + strand at 1310.632 kb on + strand, within EX28DRAFT_1302 at 1310.687 kb on + strand, within EX28DRAFT_1302 at 1310.688 kb on - strand, within EX28DRAFT_1302 at 1310.699 kb on + strand, within EX28DRAFT_1302 at 1310.781 kb on - strand, within EX28DRAFT_1302 at 1310.781 kb on - strand, within EX28DRAFT_1302 at 1311.046 kb on + strand at 1311.069 kb on - strand at 1311.084 kb on - strand at 1311.144 kb on - strand, within EX28DRAFT_1303 at 1311.186 kb on + strand, within EX28DRAFT_1303 at 1311.246 kb on + strand at 1311.336 kb on + strand, within EX28DRAFT_1304 at 1311.379 kb on + strand, within EX28DRAFT_1304 at 1311.498 kb on + strand, within EX28DRAFT_1304 at 1311.502 kb on + strand, within EX28DRAFT_1304 at 1311.502 kb on + strand, within EX28DRAFT_1304 at 1311.517 kb on + strand, within EX28DRAFT_1304 at 1311.539 kb on - strand, within EX28DRAFT_1304 at 1311.539 kb on - strand, within EX28DRAFT_1304 at 1311.602 kb on + strand, within EX28DRAFT_1304 at 1311.647 kb on - strand, within EX28DRAFT_1304 at 1311.789 kb on - strand at 1311.846 kb on - strand at 1312.029 kb on - strand at 1312.071 kb on + strand, within EX28DRAFT_1305 at 1312.115 kb on + strand, within EX28DRAFT_1305 at 1312.143 kb on + strand, within EX28DRAFT_1305
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,310,101 + -3.8 1,310,146 - EX28DRAFT_1300 0.18 -2.2 1,310,238 - EX28DRAFT_1300 0.84 -1.2 1,310,285 + EX28DRAFT_1301 0.12 -0.6 1,310,286 - EX28DRAFT_1301 0.13 -1.3 1,310,286 - EX28DRAFT_1301 0.13 -0.3 1,310,286 - EX28DRAFT_1301 0.13 +1.0 1,310,294 + EX28DRAFT_1301 0.16 -0.1 1,310,400 + EX28DRAFT_1301 0.64 +1.4 1,310,461 + -3.2 1,310,462 - -3.0 1,310,462 - +0.3 1,310,465 + +0.0 1,310,466 - -0.1 1,310,466 - -0.7 1,310,475 + +0.3 1,310,632 + EX28DRAFT_1302 0.25 +0.3 1,310,687 + EX28DRAFT_1302 0.34 -0.1 1,310,688 - EX28DRAFT_1302 0.34 -2.2 1,310,699 + EX28DRAFT_1302 0.36 +0.1 1,310,781 - EX28DRAFT_1302 0.50 +0.7 1,310,781 - EX28DRAFT_1302 0.50 -3.2 1,311,046 + +0.9 1,311,069 - +0.7 1,311,084 - +0.7 1,311,144 - EX28DRAFT_1303 0.36 -1.2 1,311,186 + EX28DRAFT_1303 0.61 -0.2 1,311,246 + -0.6 1,311,336 + EX28DRAFT_1304 0.20 -0.4 1,311,379 + EX28DRAFT_1304 0.30 -0.6 1,311,498 + EX28DRAFT_1304 0.56 -2.0 1,311,502 + EX28DRAFT_1304 0.57 +0.3 1,311,502 + EX28DRAFT_1304 0.57 -0.1 1,311,517 + EX28DRAFT_1304 0.60 +0.1 1,311,539 - EX28DRAFT_1304 0.65 -1.0 1,311,539 - EX28DRAFT_1304 0.65 -3.4 1,311,602 + EX28DRAFT_1304 0.79 +2.1 1,311,647 - EX28DRAFT_1304 0.89 -1.3 1,311,789 - +1.2 1,311,846 - +0.2 1,312,029 - +1.5 1,312,071 + EX28DRAFT_1305 0.14 -2.0 1,312,115 + EX28DRAFT_1305 0.20 -1.6 1,312,143 + EX28DRAFT_1305 0.23 -2.5
Or see this region's nucleotide sequence