Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1234 and EX28DRAFT_1235 are separated by 22 nucleotides EX28DRAFT_1235 and EX28DRAFT_1236 are separated by 114 nucleotides EX28DRAFT_1236 and EX28DRAFT_1237 overlap by 14 nucleotides
EX28DRAFT_1234: EX28DRAFT_1234 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 1,253,001 to 1,253,774
_1234
EX28DRAFT_1235: EX28DRAFT_1235 - Aspartate/tyrosine/aromatic aminotransferase, at 1,253,797 to 1,254,966
_1235
EX28DRAFT_1236: EX28DRAFT_1236 - Transcriptional regulator, at 1,255,081 to 1,255,923
_1236
EX28DRAFT_1237: EX28DRAFT_1237 - Carboxylesterase type B, at 1,255,910 to 1,257,415
_1237
Position (kb)
1253
1254
1255 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1252.877 kb on - strand at 1252.932 kb on - strand at 1253.003 kb on - strand at 1253.033 kb on - strand at 1253.033 kb on - strand at 1253.036 kb on + strand at 1253.037 kb on - strand at 1253.037 kb on - strand at 1253.070 kb on + strand at 1253.071 kb on - strand at 1253.075 kb on + strand at 1253.153 kb on + strand, within EX28DRAFT_1234 at 1253.288 kb on + strand, within EX28DRAFT_1234 at 1253.300 kb on + strand, within EX28DRAFT_1234 at 1253.300 kb on + strand, within EX28DRAFT_1234 at 1253.302 kb on + strand, within EX28DRAFT_1234 at 1253.303 kb on - strand, within EX28DRAFT_1234 at 1253.303 kb on - strand, within EX28DRAFT_1234 at 1253.453 kb on + strand, within EX28DRAFT_1234 at 1253.757 kb on + strand at 1253.757 kb on + strand at 1253.776 kb on + strand at 1253.777 kb on - strand at 1253.777 kb on - strand at 1253.794 kb on - strand at 1253.920 kb on - strand, within EX28DRAFT_1235 at 1254.034 kb on - strand, within EX28DRAFT_1235 at 1254.123 kb on + strand, within EX28DRAFT_1235 at 1254.124 kb on - strand, within EX28DRAFT_1235 at 1254.124 kb on - strand, within EX28DRAFT_1235 at 1254.224 kb on + strand, within EX28DRAFT_1235 at 1254.252 kb on - strand, within EX28DRAFT_1235 at 1254.274 kb on - strand, within EX28DRAFT_1235 at 1254.453 kb on + strand, within EX28DRAFT_1235 at 1254.606 kb on - strand, within EX28DRAFT_1235 at 1254.606 kb on - strand, within EX28DRAFT_1235 at 1254.606 kb on - strand, within EX28DRAFT_1235 at 1254.730 kb on - strand, within EX28DRAFT_1235 at 1254.739 kb on - strand, within EX28DRAFT_1235 at 1254.865 kb on - strand at 1255.070 kb on + strand at 1255.071 kb on - strand at 1255.078 kb on + strand at 1255.078 kb on + strand at 1255.078 kb on + strand at 1255.079 kb on - strand at 1255.079 kb on - strand at 1255.079 kb on - strand at 1255.079 kb on - strand at 1255.080 kb on + strand at 1255.081 kb on - strand at 1255.081 kb on - strand at 1255.081 kb on - strand at 1255.572 kb on - strand, within EX28DRAFT_1236 at 1255.805 kb on + strand, within EX28DRAFT_1236 at 1255.826 kb on + strand, within EX28DRAFT_1236 at 1255.827 kb on - strand, within EX28DRAFT_1236
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,252,877 - -1.2 1,252,932 - +0.2 1,253,003 - +0.8 1,253,033 - +1.4 1,253,033 - -1.8 1,253,036 + +1.1 1,253,037 - -2.8 1,253,037 - +0.4 1,253,070 + +0.1 1,253,071 - +0.3 1,253,075 + +0.2 1,253,153 + EX28DRAFT_1234 0.20 -1.2 1,253,288 + EX28DRAFT_1234 0.37 -3.7 1,253,300 + EX28DRAFT_1234 0.39 -0.0 1,253,300 + EX28DRAFT_1234 0.39 -2.5 1,253,302 + EX28DRAFT_1234 0.39 +0.0 1,253,303 - EX28DRAFT_1234 0.39 +1.2 1,253,303 - EX28DRAFT_1234 0.39 +0.8 1,253,453 + EX28DRAFT_1234 0.58 +0.2 1,253,757 + +0.6 1,253,757 + +0.7 1,253,776 + +0.0 1,253,777 - -1.2 1,253,777 - -1.1 1,253,794 - -1.2 1,253,920 - EX28DRAFT_1235 0.11 +0.8 1,254,034 - EX28DRAFT_1235 0.20 -0.1 1,254,123 + EX28DRAFT_1235 0.28 +1.2 1,254,124 - EX28DRAFT_1235 0.28 -1.2 1,254,124 - EX28DRAFT_1235 0.28 +1.7 1,254,224 + EX28DRAFT_1235 0.36 +0.9 1,254,252 - EX28DRAFT_1235 0.39 -0.9 1,254,274 - EX28DRAFT_1235 0.41 +0.0 1,254,453 + EX28DRAFT_1235 0.56 -0.9 1,254,606 - EX28DRAFT_1235 0.69 +1.0 1,254,606 - EX28DRAFT_1235 0.69 -0.2 1,254,606 - EX28DRAFT_1235 0.69 -2.8 1,254,730 - EX28DRAFT_1235 0.80 -1.1 1,254,739 - EX28DRAFT_1235 0.81 -1.8 1,254,865 - +1.3 1,255,070 + +0.1 1,255,071 - +0.9 1,255,078 + -0.6 1,255,078 + -1.1 1,255,078 + -1.2 1,255,079 - +2.1 1,255,079 - -1.8 1,255,079 - +0.5 1,255,079 - +0.6 1,255,080 + -0.2 1,255,081 - -0.2 1,255,081 - +0.5 1,255,081 - -0.3 1,255,572 - EX28DRAFT_1236 0.58 +0.6 1,255,805 + EX28DRAFT_1236 0.86 -4.2 1,255,826 + EX28DRAFT_1236 0.88 +0.0 1,255,827 - EX28DRAFT_1236 0.88 -2.1
Or see this region's nucleotide sequence