Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1130 and EX28DRAFT_1131 are separated by 87 nucleotides EX28DRAFT_1131 and EX28DRAFT_1132 are separated by 47 nucleotides EX28DRAFT_1132 and EX28DRAFT_1133 are separated by 7 nucleotides EX28DRAFT_1133 and EX28DRAFT_1134 are separated by 41 nucleotides
EX28DRAFT_1130: EX28DRAFT_1130 - RNA polymerase sigma factor, sigma-70 family, at 1,144,792 to 1,145,352
_1130
EX28DRAFT_1131: EX28DRAFT_1131 - hypothetical protein, at 1,145,440 to 1,145,811
_1131
EX28DRAFT_1132: EX28DRAFT_1132 - Thiol:disulfide interchange protein, at 1,145,859 to 1,147,883
_1132
EX28DRAFT_1133: EX28DRAFT_1133 - Protein-disulfide isomerase, at 1,147,891 to 1,148,502
_1133
EX28DRAFT_1134: EX28DRAFT_1134 - Protein-disulfide isomerase, at 1,148,544 to 1,149,302
_1134
Position (kb)
1145
1146
1147
1148 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1144.925 kb on - strand, within EX28DRAFT_1130 at 1145.055 kb on - strand, within EX28DRAFT_1130 at 1145.129 kb on - strand, within EX28DRAFT_1130 at 1145.232 kb on + strand, within EX28DRAFT_1130 at 1145.348 kb on + strand at 1145.721 kb on + strand, within EX28DRAFT_1131 at 1145.721 kb on + strand, within EX28DRAFT_1131 at 1145.780 kb on + strand at 1145.780 kb on + strand at 1145.781 kb on - strand at 1146.025 kb on + strand at 1146.026 kb on - strand at 1146.045 kb on + strand at 1146.045 kb on + strand at 1146.144 kb on - strand, within EX28DRAFT_1132 at 1146.333 kb on - strand, within EX28DRAFT_1132 at 1146.359 kb on + strand, within EX28DRAFT_1132 at 1146.456 kb on + strand, within EX28DRAFT_1132 at 1146.457 kb on - strand, within EX28DRAFT_1132 at 1146.457 kb on - strand, within EX28DRAFT_1132 at 1146.457 kb on - strand, within EX28DRAFT_1132 at 1146.932 kb on + strand, within EX28DRAFT_1132 at 1147.274 kb on - strand, within EX28DRAFT_1132 at 1147.330 kb on + strand, within EX28DRAFT_1132 at 1147.331 kb on - strand, within EX28DRAFT_1132 at 1147.608 kb on - strand, within EX28DRAFT_1132 at 1147.887 kb on + strand at 1147.887 kb on + strand at 1147.887 kb on + strand at 1147.888 kb on - strand at 1147.888 kb on - strand at 1147.909 kb on - strand at 1148.077 kb on + strand, within EX28DRAFT_1133 at 1148.077 kb on + strand, within EX28DRAFT_1133 at 1148.094 kb on + strand, within EX28DRAFT_1133 at 1148.110 kb on + strand, within EX28DRAFT_1133 at 1148.158 kb on - strand, within EX28DRAFT_1133 at 1148.241 kb on + strand, within EX28DRAFT_1133 at 1148.558 kb on - strand at 1148.560 kb on + strand at 1148.561 kb on - strand at 1148.649 kb on - strand, within EX28DRAFT_1134 at 1148.649 kb on - strand, within EX28DRAFT_1134 at 1148.820 kb on + strand, within EX28DRAFT_1134
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,144,925 - EX28DRAFT_1130 0.24 -1.2 1,145,055 - EX28DRAFT_1130 0.47 +0.6 1,145,129 - EX28DRAFT_1130 0.60 -1.2 1,145,232 + EX28DRAFT_1130 0.78 -0.4 1,145,348 + -0.2 1,145,721 + EX28DRAFT_1131 0.76 +0.2 1,145,721 + EX28DRAFT_1131 0.76 -3.7 1,145,780 + -2.5 1,145,780 + -0.5 1,145,781 - +0.2 1,146,025 + +0.4 1,146,026 - +0.4 1,146,045 + -0.1 1,146,045 + +0.3 1,146,144 - EX28DRAFT_1132 0.14 +2.2 1,146,333 - EX28DRAFT_1132 0.23 -0.3 1,146,359 + EX28DRAFT_1132 0.25 -0.0 1,146,456 + EX28DRAFT_1132 0.29 -0.2 1,146,457 - EX28DRAFT_1132 0.30 +0.2 1,146,457 - EX28DRAFT_1132 0.30 +0.6 1,146,457 - EX28DRAFT_1132 0.30 +0.5 1,146,932 + EX28DRAFT_1132 0.53 +0.2 1,147,274 - EX28DRAFT_1132 0.70 +0.4 1,147,330 + EX28DRAFT_1132 0.73 +0.6 1,147,331 - EX28DRAFT_1132 0.73 +0.8 1,147,608 - EX28DRAFT_1132 0.86 +1.6 1,147,887 + +0.5 1,147,887 + +0.2 1,147,887 + -0.3 1,147,888 - +0.1 1,147,888 - -0.3 1,147,909 - +1.3 1,148,077 + EX28DRAFT_1133 0.30 +1.1 1,148,077 + EX28DRAFT_1133 0.30 -0.2 1,148,094 + EX28DRAFT_1133 0.33 -0.4 1,148,110 + EX28DRAFT_1133 0.36 -1.7 1,148,158 - EX28DRAFT_1133 0.44 +0.4 1,148,241 + EX28DRAFT_1133 0.57 +1.2 1,148,558 - +1.4 1,148,560 + -0.4 1,148,561 - +0.4 1,148,649 - EX28DRAFT_1134 0.14 -0.8 1,148,649 - EX28DRAFT_1134 0.14 +1.7 1,148,820 + EX28DRAFT_1134 0.36 +0.7
Or see this region's nucleotide sequence