Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0846 and EX28DRAFT_0847 are separated by 47 nucleotides EX28DRAFT_0847 and EX28DRAFT_0848 are separated by 3 nucleotides EX28DRAFT_0848 and EX28DRAFT_0849 overlap by 8 nucleotides
EX28DRAFT_0846: EX28DRAFT_0846 - Anaerobic dehydrogenases, typically selenocysteine-containing, at 852,292 to 854,817
_0846
EX28DRAFT_0847: EX28DRAFT_0847 - Protein of unknown function (DUF3442), at 854,865 to 856,271
_0847
EX28DRAFT_0848: EX28DRAFT_0848 - two component transcriptional regulator, LuxR family, at 856,275 to 856,925
_0848
EX28DRAFT_0849: EX28DRAFT_0849 - Signal transduction histidine kinase, nitrate/nitrite-specific, at 856,918 to 858,714
_0849
Position (kb) 854
855
856
857 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 853.900 kb on + strand, within EX28DRAFT_0846 at 853.945 kb on - strand, within EX28DRAFT_0846 at 853.977 kb on + strand, within EX28DRAFT_0846 at 853.978 kb on - strand, within EX28DRAFT_0846 at 854.284 kb on + strand, within EX28DRAFT_0846 at 854.284 kb on + strand, within EX28DRAFT_0846 at 854.284 kb on + strand, within EX28DRAFT_0846 at 854.285 kb on - strand, within EX28DRAFT_0846 at 854.285 kb on - strand, within EX28DRAFT_0846 at 854.285 kb on - strand, within EX28DRAFT_0846 at 854.389 kb on + strand, within EX28DRAFT_0846 at 854.390 kb on - strand, within EX28DRAFT_0846 at 854.820 kb on + strand at 854.820 kb on + strand at 854.831 kb on + strand at 854.832 kb on - strand at 854.851 kb on + strand at 854.856 kb on - strand at 854.872 kb on + strand at 854.873 kb on - strand at 854.896 kb on + strand at 854.896 kb on + strand at 854.897 kb on - strand at 854.897 kb on - strand at 854.897 kb on - strand at 854.899 kb on + strand at 854.899 kb on + strand at 854.948 kb on + strand at 854.976 kb on - strand at 854.979 kb on - strand at 855.218 kb on - strand, within EX28DRAFT_0847 at 855.400 kb on - strand, within EX28DRAFT_0847 at 855.483 kb on + strand, within EX28DRAFT_0847 at 855.483 kb on + strand, within EX28DRAFT_0847 at 855.484 kb on - strand, within EX28DRAFT_0847 at 855.484 kb on - strand, within EX28DRAFT_0847 at 855.484 kb on - strand, within EX28DRAFT_0847 at 855.537 kb on - strand, within EX28DRAFT_0847 at 855.618 kb on + strand, within EX28DRAFT_0847 at 855.618 kb on + strand, within EX28DRAFT_0847 at 855.696 kb on + strand, within EX28DRAFT_0847 at 855.696 kb on + strand, within EX28DRAFT_0847 at 855.696 kb on + strand, within EX28DRAFT_0847 at 855.697 kb on - strand, within EX28DRAFT_0847 at 855.697 kb on - strand, within EX28DRAFT_0847 at 855.697 kb on - strand, within EX28DRAFT_0847 at 855.765 kb on - strand, within EX28DRAFT_0847 at 855.765 kb on - strand, within EX28DRAFT_0847 at 855.974 kb on + strand, within EX28DRAFT_0847 at 856.225 kb on + strand at 856.226 kb on - strand at 856.269 kb on + strand at 856.270 kb on - strand at 856.270 kb on - strand at 856.346 kb on + strand, within EX28DRAFT_0848 at 856.347 kb on - strand, within EX28DRAFT_0848 at 856.347 kb on - strand, within EX28DRAFT_0848 at 856.743 kb on - strand, within EX28DRAFT_0848 at 856.948 kb on - strand at 856.965 kb on + strand at 856.965 kb on + strand at 857.052 kb on + strand at 857.053 kb on - strand at 857.218 kb on + strand, within EX28DRAFT_0849 at 857.218 kb on + strand, within EX28DRAFT_0849 at 857.219 kb on - strand, within EX28DRAFT_0849 at 857.224 kb on + strand, within EX28DRAFT_0849 at 857.224 kb on + strand, within EX28DRAFT_0849 at 857.224 kb on + strand, within EX28DRAFT_0849 at 857.225 kb on - strand, within EX28DRAFT_0849 at 857.225 kb on - strand, within EX28DRAFT_0849 at 857.225 kb on - strand, within EX28DRAFT_0849
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 853,900 + EX28DRAFT_0846 0.64 +0.7 853,945 - EX28DRAFT_0846 0.65 +0.9 853,977 + EX28DRAFT_0846 0.67 +0.2 853,978 - EX28DRAFT_0846 0.67 -1.8 854,284 + EX28DRAFT_0846 0.79 +0.2 854,284 + EX28DRAFT_0846 0.79 +1.6 854,284 + EX28DRAFT_0846 0.79 -0.2 854,285 - EX28DRAFT_0846 0.79 -2.5 854,285 - EX28DRAFT_0846 0.79 +0.4 854,285 - EX28DRAFT_0846 0.79 +0.5 854,389 + EX28DRAFT_0846 0.83 -0.5 854,390 - EX28DRAFT_0846 0.83 +0.1 854,820 + -2.8 854,820 + +0.8 854,831 + -1.0 854,832 - +1.9 854,851 + -3.0 854,856 - +0.6 854,872 + -0.0 854,873 - -0.3 854,896 + +1.1 854,896 + +0.5 854,897 - +0.0 854,897 - -2.5 854,897 - -0.8 854,899 + -2.7 854,899 + +0.8 854,948 + +1.2 854,976 - -2.3 854,979 - -2.5 855,218 - EX28DRAFT_0847 0.25 -2.5 855,400 - EX28DRAFT_0847 0.38 -1.2 855,483 + EX28DRAFT_0847 0.44 -3.7 855,483 + EX28DRAFT_0847 0.44 +0.2 855,484 - EX28DRAFT_0847 0.44 -0.6 855,484 - EX28DRAFT_0847 0.44 -0.0 855,484 - EX28DRAFT_0847 0.44 +0.6 855,537 - EX28DRAFT_0847 0.48 +1.2 855,618 + EX28DRAFT_0847 0.54 +1.1 855,618 + EX28DRAFT_0847 0.54 -0.4 855,696 + EX28DRAFT_0847 0.59 +1.4 855,696 + EX28DRAFT_0847 0.59 -0.7 855,696 + EX28DRAFT_0847 0.59 -1.3 855,697 - EX28DRAFT_0847 0.59 -0.2 855,697 - EX28DRAFT_0847 0.59 -0.8 855,697 - EX28DRAFT_0847 0.59 +0.8 855,765 - EX28DRAFT_0847 0.64 -2.7 855,765 - EX28DRAFT_0847 0.64 -0.7 855,974 + EX28DRAFT_0847 0.79 -0.2 856,225 + -1.0 856,226 - -0.5 856,269 + +0.9 856,270 - +3.0 856,270 - -1.3 856,346 + EX28DRAFT_0848 0.11 -2.8 856,347 - EX28DRAFT_0848 0.11 +0.3 856,347 - EX28DRAFT_0848 0.11 +1.3 856,743 - EX28DRAFT_0848 0.72 +0.0 856,948 - +0.4 856,965 + -1.2 856,965 + -2.5 857,052 + -0.3 857,053 - -0.0 857,218 + EX28DRAFT_0849 0.17 +1.6 857,218 + EX28DRAFT_0849 0.17 +0.9 857,219 - EX28DRAFT_0849 0.17 -0.6 857,224 + EX28DRAFT_0849 0.17 -0.1 857,224 + EX28DRAFT_0849 0.17 -0.7 857,224 + EX28DRAFT_0849 0.17 -0.3 857,225 - EX28DRAFT_0849 0.17 -0.4 857,225 - EX28DRAFT_0849 0.17 +0.9 857,225 - EX28DRAFT_0849 0.17 +0.1
Or see this region's nucleotide sequence